##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550735_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414091
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.156219285132977	31.0	31.0	33.0	28.0	34.0
2	31.142374502222943	31.0	31.0	34.0	28.0	34.0
3	30.980581562989777	31.0	31.0	34.0	27.0	34.0
4	34.8597916883004	35.0	35.0	37.0	32.0	37.0
5	34.84021869589051	35.0	35.0	37.0	33.0	37.0
6	35.011487813065244	36.0	35.0	37.0	32.0	37.0
7	35.449664445737774	37.0	35.0	37.0	33.0	37.0
8	34.99490933152375	37.0	35.0	37.0	32.0	37.0
9	36.798679517304166	39.0	37.0	39.0	32.0	39.0
10	36.28298127706229	37.0	35.0	39.0	32.0	39.0
11	36.66149228068227	38.0	35.0	39.0	32.0	39.0
12	36.812024410093436	39.0	35.0	39.0	33.0	39.0
13	36.82388895194534	39.0	37.0	39.0	33.0	39.0
14	37.85799498177937	40.0	37.0	41.0	33.0	41.0
15	38.02476750279528	40.0	37.0	41.0	33.0	41.0
16	38.03664170435967	40.0	37.0	41.0	33.0	41.0
17	37.91452361920447	39.0	37.0	41.0	33.0	41.0
18	37.92254118056176	39.0	37.0	41.0	33.0	41.0
19	37.82657435201441	39.0	37.0	41.0	33.0	41.0
20	37.84767599392404	39.0	37.0	41.0	33.0	41.0
21	37.873254912567525	39.0	37.0	41.0	33.0	41.0
22	37.99741361198384	40.0	37.0	41.0	33.0	41.0
23	38.02165224552091	40.0	37.0	41.0	34.0	41.0
24	38.00049989012077	40.0	37.0	41.0	33.0	41.0
25	37.64922927569061	39.0	36.0	41.0	33.0	41.0
26	37.70256779306964	39.0	36.0	41.0	33.0	41.0
27	37.743467015704276	40.0	36.0	41.0	33.0	41.0
28	37.65037636654745	40.0	36.0	41.0	33.0	41.0
29	37.620025549939506	40.0	36.0	41.0	33.0	41.0
30	37.40981330190707	40.0	36.0	41.0	32.0	41.0
31	37.393589814799164	39.0	36.0	41.0	32.0	41.0
32	37.389851506069924	40.0	36.0	41.0	33.0	41.0
33	37.413153147496566	40.0	36.0	41.0	33.0	41.0
34	37.27731585569356	40.0	36.0	41.0	32.0	41.0
35	37.32375009357847	40.0	36.0	41.0	32.0	41.0
36	37.23554725893584	40.0	36.0	41.0	32.0	41.0
37	37.25839489387598	40.0	36.0	41.0	32.0	41.0
38	37.06981798686762	39.0	35.0	41.0	32.0	41.0
39	36.99188101166169	39.0	35.0	41.0	31.0	41.0
40	36.889364898053806	39.0	35.0	41.0	31.0	41.0
41	36.7005247638804	39.0	35.0	41.0	31.0	41.0
42	36.83243779748896	39.0	35.0	41.0	31.0	41.0
43	36.80204592710298	39.0	35.0	41.0	31.0	41.0
44	36.84717368887515	39.0	35.0	41.0	31.0	41.0
45	36.833604207770755	39.0	35.0	41.0	31.0	41.0
46	36.70893112866496	39.0	35.0	41.0	31.0	41.0
47	36.62777022441927	39.0	35.0	41.0	31.0	41.0
48	36.58256035509103	39.0	35.0	41.0	31.0	41.0
49	36.57036738301485	39.0	35.0	41.0	31.0	41.0
50	36.44061329514527	39.0	35.0	41.0	31.0	41.0
51	35.266238580408654	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	19.0
10	12.0
11	21.0
12	13.0
13	4.0
14	15.0
15	24.0
16	41.0
17	63.0
18	124.0
19	248.0
20	389.0
21	776.0
22	1225.0
23	1640.0
24	2069.0
25	2434.0
26	3068.0
27	3406.0
28	4114.0
29	4897.0
30	6328.0
31	8112.0
32	11067.0
33	15767.0
34	26695.0
35	29824.0
36	34231.0
37	53124.0
38	95725.0
39	108587.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.35432549850154	20.167306220130357	21.686537500211305	10.791830781156799
2	32.95459210656595	27.177118073080553	23.226537162121367	16.64175265823213
3	32.827808380283564	26.43694260440338	24.48423172684265	16.25101728847041
4	29.13031193626522	24.234286666457372	27.463045562448833	19.172355834828576
5	27.02908297934512	29.551958385958642	23.474791772822883	19.944166861873356
6	24.180675262200822	37.329234395338226	23.926383331200146	14.56370701126081
7	74.64397922195846	6.686452977727118	13.388844481044021	5.280723319270402
8	72.74777766239788	10.155497221625199	10.639690309617935	6.457034806358989
9	65.62277373813968	9.321622541905041	13.247571186043647	11.808032533911629
10	38.15658876913528	24.720170204133872	21.34796457783434	15.7752764488965
11	30.17887372582355	26.039203943094634	24.278479851047233	19.503442480034582
12	26.988512186934756	21.948798694006875	29.618610402061385	21.44407871699699
13	25.229961530195055	23.40403437891671	31.846864578075834	19.5191395128124
14	20.179622353540648	29.42831406623182	28.27059752566465	22.121466054562884
15	18.217734749125192	25.75786481715372	35.41612833893999	20.6082720947811
16	20.396241405874555	26.40409958197594	30.326184341123085	22.87347467102642
17	20.49935883658423	26.497074314583024	29.611607110514353	23.391959738318388
18	21.53657046398014	25.908073346196854	29.966843036917005	22.588513152906003
19	22.60783257786332	27.733759004663227	27.281201475038095	22.37720694243536
20	24.733210815980062	26.82308961073774	28.98928013407681	19.45441943920539
21	22.609523027547084	28.740542537751363	28.69369293222987	19.956241502471677
22	20.137844097070452	25.04521952904072	29.188270211137162	25.628666162751667
23	21.196790077543344	27.81248566136429	29.080322924188163	21.910401336904208
24	21.981400223622344	25.803748451427346	28.303440548092084	23.911410776858226
25	20.71501191767027	29.27182672407756	26.13797450318891	23.87518685506326
26	19.691565380556447	27.77167337614196	28.87046567058931	23.66629557271228
27	23.068842355907275	27.340608706781843	26.643418958634697	22.947129978676184
28	19.392114293718045	28.135844536587367	29.112924453803636	23.359116715890952
29	20.824891147115007	28.653605125443445	27.56133313691918	22.96017059052237
30	22.51316739557247	26.94262855266113	27.315493454337332	23.228710597429068
31	23.389061824574792	27.716130029389674	25.188183273724857	23.706624872310673
32	23.67233289301144	30.210509284191154	24.334747676235416	21.782410146561986
33	24.155077024132375	25.850115071324904	25.533035009212952	24.46177289532977
34	22.246800823973476	26.98464830194329	28.10565793509156	22.66289293899167
35	21.063727538149827	25.731783593461337	26.717557251908396	26.486931616480437
36	22.788469201214227	27.884933504954223	27.234351869516605	22.092245424314946
37	20.95408980151706	27.688116863201568	28.01195872404858	23.345834611232796
38	23.067393399035478	26.559862445694304	25.60427538874305	24.768468766527164
39	23.045417553146528	25.266668437613955	29.095053985718117	22.5928600235214
40	23.245856587078688	23.083814910249195	30.155448923062806	23.514879579609314
41	19.196263623213255	25.67744771076889	29.986162461874322	25.14012620414353
42	20.866669403585203	25.146646510066628	29.12548208002589	24.86120200632228
43	22.92032427654791	24.90829310465574	28.690795018486277	23.480587600310077
44	21.796899715279974	25.10124586141694	28.314307724630577	24.787546698672514
45	21.956767956801766	23.70903980043034	27.153451777507843	27.18074046526005
46	23.582256074147953	26.074461893641736	27.035361792456246	23.30792023975406
47	20.032311738241113	25.006097693502156	31.66333004098133	23.298260527275406
48	20.881400465115156	24.906602654971973	28.419598590647947	25.792398289264916
49	21.26634000738968	23.503770910258854	31.615997449835902	23.613891632515557
50	20.960851600252116	23.04879845251406	30.088072428524164	25.902277518709653
51	19.93981999125796	23.040346204095236	27.99746915533059	29.022364649316213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157.0
1	440.5
2	724.0
3	1424.5
4	2125.0
5	1460.5
6	796.0
7	775.5
8	755.0
9	725.0
10	695.0
11	708.5
12	722.0
13	680.0
14	638.0
15	658.5
16	679.0
17	743.5
18	808.0
19	993.5
20	1179.0
21	1298.5
22	1418.0
23	1587.0
24	1756.0
25	2134.5
26	3057.0
27	3601.0
28	4194.5
29	4788.0
30	6295.0
31	7802.0
32	8237.0
33	8672.0
34	10077.5
35	11483.0
36	12103.0
37	12723.0
38	13597.5
39	14472.0
40	15912.5
41	17353.0
42	19632.5
43	21912.0
44	24322.0
45	26732.0
46	37612.0
47	48492.0
48	46090.0
49	43688.0
50	42440.0
51	41192.0
52	35630.0
53	30068.0
54	26464.5
55	22861.0
56	20459.5
57	18058.0
58	16703.5
59	15349.0
60	15378.5
61	15408.0
62	13888.0
63	12368.0
64	10238.5
65	8109.0
66	6476.0
67	4843.0
68	4142.5
69	3442.0
70	2931.5
71	2421.0
72	1817.5
73	1214.0
74	1020.5
75	720.0
76	613.0
77	464.5
78	316.0
79	227.5
80	139.0
81	112.0
82	85.0
83	70.5
84	56.0
85	37.5
86	19.0
87	17.0
88	15.0
89	8.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	414091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.391959172010395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5215365072629	25.666792591565102
2	10.785297872659157	7.63425643934247
3	3.7603428937758103	3.992577065078187
4	1.9673176315983496	2.7850890118361993
5	1.2683203065158382	2.244417025262013
6	0.9809499245257576	2.0830643807161375
7	0.8239362828355156	2.0412503497716803
8	0.7038537996452723	1.992861195208789
9	0.651201342917917	2.0742562207178334
>10	6.427705443087615	40.872435894157
>50	0.06613614803068932	1.6401882471852796
>100	0.0351348286413037	2.154895752731307
>500	0.004133509251918082	1.0518466029964764
>1k	0.004133509251918082	3.7660692234315194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC	4003	0.9666957263017066	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	3729	0.9005266958228989	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	3252	0.7853346245149013	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2190	0.5288692582065294	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1265	0.30548840713756154	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	1007	0.24318326165021698	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	871	0.21034023922277953	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT	762	0.18401752271843627	No Hit
GAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	737	0.17798020241927498	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGAGTCTT	708	0.17097691087224787	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTA	622	0.15020852904313303	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTC	614	0.14827658654740142	No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	454	0.10963773663276913	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.414928119664518E-4	0.0	0.0	0.2175850235817731	0.0
2	2.414928119664518E-4	0.0	0.0	1.2231610926100784	0.0
3	2.414928119664518E-4	0.0	0.0	1.598682415217911	0.0
4	2.414928119664518E-4	0.0	0.0	2.2813825946470705	0.0
5	2.414928119664518E-4	0.0	0.0	4.534993515917999	0.0
6	2.414928119664518E-4	0.0	0.0	5.427792441757971	0.0
7	2.414928119664518E-4	0.0	0.0	6.411634157709296	0.0
8	2.414928119664518E-4	0.0	0.0	7.8461014607900195	0.0
9	2.414928119664518E-4	0.0	0.0	8.311458109449372	0.0
10	2.414928119664518E-4	0.0	0.0	10.166122905351722	0.0
11	2.414928119664518E-4	0.0	0.0	11.73993156093709	0.0
12	2.414928119664518E-4	0.0	0.0	13.995232931891783	0.0
13	2.414928119664518E-4	0.0	0.0	14.597274512124146	0.0
14	2.414928119664518E-4	0.0	0.0	14.855188835304316	0.0
15	2.414928119664518E-4	0.0	0.0	15.443948310878527	0.0
16	2.414928119664518E-4	0.0	0.0	16.3727296657015	0.0
17	2.414928119664518E-4	0.0	0.0	17.34401375543057	0.0
18	2.414928119664518E-4	0.0	0.0	18.39426599467267	0.0
19	2.414928119664518E-4	0.0	0.0	19.19795407289702	0.0
20	2.414928119664518E-4	0.0	0.0	19.84443033053121	0.0
21	2.414928119664518E-4	0.0	0.0	20.643530045328202	0.0
22	2.414928119664518E-4	0.0	0.0	21.49092832251848	0.0
23	2.414928119664518E-4	0.0	0.0	22.26491278487096	0.0
24	2.414928119664518E-4	0.0	0.0	22.88554931162474	0.0
25	2.414928119664518E-4	0.0	0.0	23.41755797638683	0.0
26	2.414928119664518E-4	0.0	0.0	23.920346010900985	0.0
27	2.414928119664518E-4	0.0	0.0	24.463463345013537	0.0
28	2.414928119664518E-4	0.0	0.0	25.00778814318592	0.0
29	2.414928119664518E-4	0.0	0.0	25.56370459633269	0.0
30	2.414928119664518E-4	0.0	0.0	26.17830380278731	0.0
31	2.414928119664518E-4	0.0	0.0	26.74315548997684	0.0
32	2.414928119664518E-4	0.0	0.0	27.29110268032872	0.0
33	2.414928119664518E-4	0.0	0.0	27.853056453774652	0.0
34	2.414928119664518E-4	0.0	0.0	28.400279165690634	0.0
35	2.414928119664518E-4	0.0	0.0	28.969719216307528	0.0
36	2.414928119664518E-4	0.0	0.0	29.550267936274878	0.0
37	2.414928119664518E-4	0.0	0.0	30.09531721288316	0.0
38	2.414928119664518E-4	0.0	0.0	30.64592082416667	0.0
39	2.414928119664518E-4	0.0	0.0	31.224054616014353	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACGG	45	3.8380676E-10	45.000004	2
GATCCGC	25	3.8864688E-5	45.0	17
TCTTACG	20	7.0280524E-4	45.0	37
GCCGCAT	25	3.8864688E-5	45.0	19
TGGCACG	20	7.0280524E-4	45.0	1
GTTAACG	20	7.0280524E-4	45.0	1
CTCGAAT	25	3.8864688E-5	45.0	43
CATCGCC	25	3.8864688E-5	45.0	23
ATACGGA	20	7.0280524E-4	45.0	28
ATCGCCA	25	3.8864688E-5	45.0	24
AGTCGAG	20	7.0280524E-4	45.0	1
ATATGCG	30	2.1621254E-6	44.999996	1
TCGGACG	30	2.1621254E-6	44.999996	1
CTATTGG	30	2.1621254E-6	44.999996	2
TAAGGGT	85	0.0	42.35294	4
TATGCGG	75	0.0	42.0	2
CGAATAT	75	0.0	42.0	14
TACGGCT	465	0.0	41.6129	7
CGACGGT	65	0.0	41.53846	28
CGGTCTA	65	0.0	41.53846	31
>>END_MODULE