SRR3550732_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550732_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354470
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4799	1.3538522300899936	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2306	0.6505487065195926	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	1996	0.5630941969701244	RNA PCR Primer, Index 30 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	1938	0.54673174034474	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	1887	0.5323440629672469	No Hit
CGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	1401	0.39523796089937085	RNA PCR Primer, Index 30 (95% over 23bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1020	0.2877535475498632	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	958	0.27026264563996955	Illumina Single End Adapter 2 (95% over 22bp)
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	911	0.25700341354698564	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	907	0.2558749682624764	No Hit
CGTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	879	0.2479758512709115	RNA PCR Primer, Index 30 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTT	836	0.2358450644624369	No Hit
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	829	0.23387028521454564	RNA PCR Primer, Index 30 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	734	0.2070697097074506	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	580	0.16362456625384378	No Hit
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	562	0.15854656247355206	No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	535	0.1509295568031145	RNA PCR Primer, Index 30 (96% over 25bp)
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	510	0.1438767737749316	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC	441	0.12441109261714672	No Hit
TGTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTT	437	0.12328264733263747	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	415	0.11707619826783648	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	392	0.1105876378819082	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	369	0.10409907749597991	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATT	367	0.1035348548537253	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	361	0.10184218692696138	No Hit

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	25	3.885328E-5	45.000004	38
CCGGGAC	25	3.885328E-5	45.000004	5
CGAAAGG	25	3.885328E-5	45.000004	2
TACGGGT	25	3.885328E-5	45.000004	4
ATGGTCG	25	3.885328E-5	45.000004	44
CCAGTCG	25	3.885328E-5	45.000004	28
ATCTCGG	20	7.0266786E-4	45.0	10
TAGACCG	20	7.0266786E-4	45.0	15
TACGGGC	20	7.0266786E-4	45.0	4
ACGGGCC	20	7.0266786E-4	45.0	5
CCATCGA	20	7.0266786E-4	45.0	31
TCGTCTA	20	7.0266786E-4	45.0	34
AAATTCG	20	7.0266786E-4	45.0	36
TTGCGAT	20	7.0266786E-4	45.0	12
ATGCGCG	20	7.0266786E-4	45.0	1
GCGCTTA	20	7.0266786E-4	45.0	10
CGCTTAG	20	7.0266786E-4	45.0	11
CCGATCA	30	2.1612395E-6	44.999996	11
CCACGCG	30	2.1612395E-6	44.999996	1
ATGGGCG	90	0.0	42.5	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content