##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550732_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354470
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8156402516433	33.0	31.0	34.0	30.0	34.0
2	31.827573560526982	33.0	31.0	34.0	30.0	34.0
3	31.901094591925975	33.0	31.0	34.0	30.0	34.0
4	35.643549524642424	37.0	35.0	37.0	33.0	37.0
5	35.68080796682371	37.0	35.0	37.0	33.0	37.0
6	35.63818094620137	37.0	35.0	37.0	33.0	37.0
7	35.89127711795074	37.0	35.0	37.0	35.0	37.0
8	35.71556408158659	37.0	35.0	37.0	35.0	37.0
9	37.503825429514485	39.0	37.0	39.0	35.0	39.0
10	36.86618613704968	39.0	37.0	39.0	32.0	39.0
11	36.89280898242446	39.0	37.0	39.0	33.0	39.0
12	36.89756820041188	39.0	35.0	39.0	33.0	39.0
13	36.84601235647587	39.0	37.0	39.0	33.0	39.0
14	37.866902699805344	40.0	37.0	41.0	33.0	41.0
15	37.96178520044009	40.0	37.0	41.0	33.0	41.0
16	37.92206956865179	40.0	37.0	41.0	33.0	41.0
17	37.86825119192033	40.0	36.0	41.0	33.0	41.0
18	37.74428019296414	39.0	36.0	41.0	33.0	41.0
19	37.55355883431602	39.0	36.0	41.0	33.0	41.0
20	37.5805540666347	39.0	35.0	41.0	33.0	41.0
21	37.530386210398625	39.0	35.0	41.0	33.0	41.0
22	37.58675205235986	39.0	35.0	41.0	33.0	41.0
23	37.57032753124383	39.0	35.0	41.0	33.0	41.0
24	37.515880046266254	39.0	35.0	41.0	33.0	41.0
25	37.314418709622814	39.0	35.0	41.0	33.0	41.0
26	37.33352046717635	39.0	35.0	41.0	33.0	41.0
27	37.35251502242785	39.0	35.0	41.0	33.0	41.0
28	37.23452760459277	39.0	35.0	41.0	33.0	41.0
29	37.178491832877256	39.0	35.0	41.0	32.0	41.0
30	36.98156966739075	39.0	35.0	41.0	32.0	41.0
31	36.94165655767766	39.0	35.0	41.0	32.0	41.0
32	36.845504556097836	39.0	35.0	41.0	31.0	41.0
33	36.67455920106074	39.0	35.0	41.0	31.0	41.0
34	36.51881118289277	39.0	35.0	41.0	30.0	41.0
35	36.435520636443144	39.0	35.0	41.0	30.0	41.0
36	36.33973537958078	39.0	35.0	41.0	30.0	41.0
37	36.300699636076395	39.0	35.0	41.0	30.0	41.0
38	36.216650210172936	39.0	35.0	41.0	30.0	41.0
39	36.119931164837645	39.0	35.0	41.0	30.0	41.0
40	36.00989928625836	39.0	35.0	41.0	29.0	41.0
41	35.89062261968573	39.0	35.0	41.0	29.0	41.0
42	35.93109148870144	39.0	35.0	41.0	29.0	41.0
43	35.89460603154004	39.0	35.0	41.0	29.0	41.0
44	35.69643411290095	38.0	35.0	40.0	28.0	41.0
45	35.65878917820972	38.0	35.0	40.0	28.0	41.0
46	35.510810505825596	38.0	35.0	40.0	27.0	41.0
47	35.39758230597794	38.0	35.0	40.0	27.0	41.0
48	35.294402911388836	38.0	35.0	40.0	26.0	41.0
49	35.2462465088724	38.0	35.0	40.0	27.0	41.0
50	35.10707253082066	38.0	34.0	40.0	26.0	41.0
51	33.908821621011654	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	9.0
11	19.0
12	15.0
13	11.0
14	23.0
15	36.0
16	55.0
17	78.0
18	158.0
19	272.0
20	484.0
21	716.0
22	1064.0
23	1482.0
24	2110.0
25	3019.0
26	4146.0
27	4900.0
28	5142.0
29	5602.0
30	6435.0
31	7922.0
32	10033.0
33	13558.0
34	23864.0
35	30123.0
36	29905.0
37	41037.0
38	70003.0
39	92195.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.43134821000366	20.68750528958727	22.959347758625555	15.921798741783508
2	36.123508336389534	25.86170903038339	22.926622845374787	15.088159787852288
3	29.962761305611195	25.760431066098683	29.154794481902556	15.122013146387564
4	27.33150901345671	23.583095889638052	31.599006968149634	17.486388128755607
5	25.241910457866673	27.413321296583632	27.565943521313514	19.778824724236184
6	24.239568933901317	35.398764352413465	26.392078314102747	13.969588399582475
7	72.83183344147601	6.903546139306569	15.312438288148503	4.95218213106892
8	74.43422574547917	7.957514035038226	12.5446441165684	5.06361610291421
9	68.89440573250204	7.797839027280165	14.303890315118345	9.003864925099444
10	37.21979293029029	27.156317883036646	20.631365136682938	14.992524049990127
11	28.573927271701415	24.587976415493554	26.941913278979886	19.896183033825146
12	25.12765537281011	21.308714418709624	32.64310096764183	20.920529240838434
13	23.740514006827095	23.113662651282198	33.97099895618811	19.1748243857026
14	19.365249527463536	29.672750867492315	30.930121025756762	20.031878579287387
15	18.066691116314498	27.720822636612407	34.34507856800293	19.867407679070162
16	21.11659660902192	24.700256721302225	33.15118345699213	21.031963212683724
17	20.50582559878128	25.88568849267921	29.893926143256127	23.71455976528338
18	20.69286540468869	27.421502524896326	29.837221767709536	22.04841030270545
19	22.235167997291732	28.966908342031765	28.139475837165346	20.658447823511157
20	24.32561288684515	25.568595367732105	29.017124157192427	21.08866758823032
21	24.505599909724378	27.999548621886195	29.047592179874176	18.447259288515248
22	21.603802860608795	24.54848083053573	29.84032499224194	24.007391316613536
23	21.033373769289362	28.320027082686828	29.508562078596213	21.138037069427597
24	22.64451152424747	26.60817558608627	29.028126498716393	21.71918639094987
25	23.60199734815358	26.677010748441337	27.74057042909132	21.980421474313765
26	19.69108810336559	27.24941461900866	29.379355093519905	23.680142184105847
27	21.32281998476599	27.145315541512684	30.150647445481987	21.381217028239345
28	19.71168222980788	26.640336276694782	31.749936524952748	21.89804496854459
29	21.635681439896185	27.705306513950408	29.207831410274494	21.451180635878917
30	21.757271419302057	26.56585888791717	31.24044347899681	20.43642621378396
31	23.80511749936525	26.620024261573615	27.010184218692697	22.56467402036844
32	23.84376675035969	29.353682963297317	26.102914209947247	20.69963607639575
33	23.47476514232516	26.47473693119305	25.98442745507377	24.06607047140802
34	21.287273958303945	28.350212994047453	26.94360594690665	23.418907100741954
35	21.38968036787316	27.69063672525178	27.43560809095269	23.484074815922362
36	22.70742234885886	29.264817897142215	25.823624002031202	22.204135751967726
37	21.605213417214433	27.854825514147883	27.67004259880949	22.869918469828193
38	21.36259768104494	29.122633791294046	26.003328913589304	23.511439614071712
39	23.02169436059469	26.182751713826274	26.803114508985242	23.99243941659379
40	22.281716365277738	26.639489942731405	30.3283775777922	20.750416114198664
41	19.41659378790871	27.804327587666094	29.854430558298304	22.924648066126892
42	20.417524755268428	28.854628036223097	28.121138601292067	22.606708607216408
43	21.51098823595791	25.228369114452565	28.122831269218835	25.137811380370696
44	22.14094281603521	25.51725110728694	29.2295539820013	23.11225209467656
45	23.74107822946935	24.624650887240104	27.203148362343782	24.431122520946765
46	23.703275312438286	26.548367986007275	27.55578751375293	22.192569187801507
47	21.3910909244788	26.477840155725453	29.9483736282337	22.182695291562048
48	20.020594126442294	27.64239568933901	29.163822044178634	23.17318814004006
49	20.28070076452168	25.90684684176376	29.948937850875957	23.863514542838605
50	19.35114396140717	24.626907777809123	31.951081896916524	24.070866363867182
51	19.791801845008038	24.637628008011962	30.02595424154371	25.544615905436284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2793.0
1	2266.0
2	1739.0
3	4397.5
4	7056.0
5	4453.0
6	1850.0
7	1722.0
8	1594.0
9	1593.5
10	1593.0
11	1564.0
12	1535.0
13	1479.0
14	1423.0
15	1408.0
16	1393.0
17	1332.0
18	1271.0
19	1301.5
20	1332.0
21	1390.5
22	1449.0
23	1629.0
24	1809.0
25	1764.5
26	2136.5
27	2553.0
28	3148.0
29	3743.0
30	4691.0
31	5639.0
32	6291.0
33	6943.0
34	7518.5
35	8094.0
36	8803.0
37	9512.0
38	10683.0
39	11854.0
40	12979.0
41	14104.0
42	15581.5
43	17059.0
44	19884.5
45	22710.0
46	29497.5
47	36285.0
48	35719.0
49	35153.0
50	33589.0
51	32025.0
52	27395.5
53	22766.0
54	20377.0
55	17988.0
56	16824.5
57	15661.0
58	15565.5
59	15470.0
60	14438.5
61	13407.0
62	12379.0
63	11351.0
64	9565.0
65	7779.0
66	6611.0
67	5443.0
68	4511.0
69	3579.0
70	3129.0
71	2679.0
72	2191.5
73	1704.0
74	1443.0
75	881.5
76	581.0
77	463.5
78	346.0
79	260.0
80	174.0
81	114.5
82	55.0
83	44.0
84	33.0
85	24.5
86	16.0
87	17.5
88	19.0
89	11.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	354470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.44467810844091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91096729071502	26.90639800519781
2	8.51570873141178	6.036731097002605
3	3.1655731101101683	3.3660815974977334
4	1.7156050841599002	2.432362798770094
5	1.2271490961361657	2.1747952351805306
6	0.9223556064945639	1.9615558544229368
7	0.8456352201420861	2.0981287722567767
8	0.6479373346007995	1.837274420749321
9	0.5961252582809539	1.901652110287165
>10	6.340572653978252	40.44436070226311
>50	0.0629594220117373	1.5214266764655704
>100	0.035066007196410655	2.656140928110984
>500	0.008766501799102664	2.32790145576546
>1k	0.005578682963065331	4.335190346029912
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4799	1.3538522300899936	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2306	0.6505487065195926	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	1996	0.5630941969701244	RNA PCR Primer, Index 30 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	1938	0.54673174034474	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	1887	0.5323440629672469	No Hit
CGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	1401	0.39523796089937085	RNA PCR Primer, Index 30 (95% over 23bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1020	0.2877535475498632	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	958	0.27026264563996955	Illumina Single End Adapter 2 (95% over 22bp)
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	911	0.25700341354698564	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	907	0.2558749682624764	No Hit
CGTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	879	0.2479758512709115	RNA PCR Primer, Index 30 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTT	836	0.2358450644624369	No Hit
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	829	0.23387028521454564	RNA PCR Primer, Index 30 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	734	0.2070697097074506	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	580	0.16362456625384378	No Hit
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	562	0.15854656247355206	No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	535	0.1509295568031145	RNA PCR Primer, Index 30 (96% over 25bp)
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	510	0.1438767737749316	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC	441	0.12441109261714672	No Hit
TGTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTT	437	0.12328264733263747	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	415	0.11707619826783648	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	392	0.1105876378819082	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	369	0.10409907749597991	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATT	367	0.1035348548537253	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	361	0.10184218692696138	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.642226422546337E-4	0.0	0.0	0.1692667926763901	0.0
2	5.642226422546337E-4	0.0	0.0	0.8454876294185686	0.0
3	5.642226422546337E-4	0.0	0.0	1.49406155669027	0.0
4	5.642226422546337E-4	0.0	0.0	2.2399638897508956	0.0
5	5.642226422546337E-4	0.0	0.0	3.834739188083618	0.0
6	5.642226422546337E-4	0.0	0.0	5.100008463339634	0.0
7	5.642226422546337E-4	0.0	0.0	5.942674979546929	0.0
8	5.642226422546337E-4	0.0	0.0	6.830761418455722	0.0
9	5.642226422546337E-4	0.0	0.0	7.158292662284538	0.0
10	5.642226422546337E-4	0.0	0.0	8.369960786526363	0.0
11	5.642226422546337E-4	0.0	0.0	9.283437244336616	0.0
12	5.642226422546337E-4	0.0	0.0	10.549552853556014	0.0
13	8.463339633819505E-4	0.0	0.0	10.895703444579231	0.0
14	8.463339633819505E-4	0.0	0.0	11.059610122154202	0.0
15	8.463339633819505E-4	0.0	0.0	11.349902671594212	0.0
16	8.463339633819505E-4	0.0	0.0	11.799588117471155	0.0
17	8.463339633819505E-4	0.0	0.0	12.32995740119051	0.0
18	0.0011284452845092673	0.0	0.0	12.946370637853697	0.0
19	0.0011284452845092673	0.0	0.0	13.39182441391373	0.0
20	0.0011284452845092673	0.0	0.0	13.752362682314441	0.0
21	0.0011284452845092673	0.0	0.0	14.225463367844952	0.0
22	0.0011284452845092673	0.0	0.0	14.685869043924733	0.0
23	0.001692667926763901	0.0	0.0	15.174203740796118	0.0
24	0.001692667926763901	0.0	0.0	15.650125539537902	0.0
25	0.001692667926763901	0.0	0.0	16.001072023020285	0.0
26	0.001692667926763901	0.0	0.0	16.360481846136487	0.0
27	0.001692667926763901	0.0	0.0	16.70155443337941	0.0
28	0.001692667926763901	0.0	0.0	17.058425254605467	0.0
29	0.001692667926763901	0.0	0.0	17.43447964566818	0.0
30	0.001692667926763901	0.0	0.0	17.811662482015404	0.0
31	0.001692667926763901	0.0	0.0	18.18743476175699	0.0
32	0.001692667926763901	0.0	0.0	18.568567156599993	0.0
33	0.001974779247891218	0.0	0.0	18.93897932124016	0.0
34	0.0022568905690185347	0.0	0.0	19.3029029254944	0.0
35	0.0022568905690185347	0.0	0.0	19.722120348689593	0.0
36	0.0022568905690185347	0.0	0.0	20.07560583406212	0.0
37	0.0022568905690185347	0.0	0.0	20.443196885491016	0.0
38	0.0022568905690185347	0.0	0.0	20.810223714277654	0.0
39	0.0022568905690185347	0.0	0.0	21.192202443084042	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	25	3.885328E-5	45.000004	38
CCGGGAC	25	3.885328E-5	45.000004	5
CGAAAGG	25	3.885328E-5	45.000004	2
TACGGGT	25	3.885328E-5	45.000004	4
ATGGTCG	25	3.885328E-5	45.000004	44
CCAGTCG	25	3.885328E-5	45.000004	28
ATCTCGG	20	7.0266786E-4	45.0	10
TAGACCG	20	7.0266786E-4	45.0	15
TACGGGC	20	7.0266786E-4	45.0	4
ACGGGCC	20	7.0266786E-4	45.0	5
CCATCGA	20	7.0266786E-4	45.0	31
TCGTCTA	20	7.0266786E-4	45.0	34
AAATTCG	20	7.0266786E-4	45.0	36
TTGCGAT	20	7.0266786E-4	45.0	12
ATGCGCG	20	7.0266786E-4	45.0	1
GCGCTTA	20	7.0266786E-4	45.0	10
CGCTTAG	20	7.0266786E-4	45.0	11
CCGATCA	30	2.1612395E-6	44.999996	11
CCACGCG	30	2.1612395E-6	44.999996	1
ATGGGCG	90	0.0	42.5	5
>>END_MODULE