SRR3550731_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550731_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233394
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC	2683	1.1495582577101382	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG	2290	0.9811734663273264	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC	2233	0.9567512446763842	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1797	0.7699426720481246	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	1018	0.43617230948524816	Illumina Single End Adapter 1 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	937	0.4014670471391724	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	657	0.28149823902928095	Illumina Single End Adapter 1 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	596	0.2553621772624832	Illumina Single End Adapter 1 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTT	596	0.2553621772624832	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT	488	0.2090884941343822	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	457	0.19580623323650134	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	431	0.18466627248344003	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT	415	0.17781091202001767	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	413	0.17695399196208986	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA	392	0.167956331353848	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC	380	0.16281481100628123	No Hit
AAAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC	304	0.130251848805025	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	246	0.1054011671251189	Illumina Single End Adapter 1 (95% over 24bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	243	0.10411578703822721	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTT	20	7.02172E-4	45.000004	38
AAATCGT	20	7.02172E-4	45.000004	12
AGATCCC	20	7.02172E-4	45.000004	10
CGAAAAC	20	7.02172E-4	45.000004	19
GCGCGTG	20	7.02172E-4	45.000004	1
ATGTGCG	20	7.02172E-4	45.000004	1
CCAATTA	20	7.02172E-4	45.000004	30
CACGCCA	40	6.7757355E-9	45.000004	27
TGTATCG	20	7.02172E-4	45.000004	1
GACCCCT	20	7.02172E-4	45.000004	21
ATCGTTG	20	7.02172E-4	45.000004	23
ATCGTAA	20	7.02172E-4	45.000004	14
ACAGGCG	40	6.7757355E-9	45.000004	33
CGTAAGG	40	6.7757355E-9	45.000004	2
ACCGTTC	20	7.02172E-4	45.000004	39
ACGTAGG	20	7.02172E-4	45.000004	2
CGCACGG	20	7.02172E-4	45.000004	2
GTCTTAC	45	3.8198777E-10	45.000004	31
TATACGG	20	7.02172E-4	45.000004	2
CGTTTTC	20	7.02172E-4	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content