##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550731_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233394
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.708214435675295	31.0	31.0	34.0	30.0	34.0
2	31.76955277342177	33.0	31.0	34.0	30.0	34.0
3	31.86316700515009	33.0	31.0	34.0	30.0	34.0
4	35.61909903425109	37.0	35.0	37.0	33.0	37.0
5	35.642934265662355	37.0	35.0	37.0	33.0	37.0
6	35.56895207246116	37.0	35.0	37.0	33.0	37.0
7	35.83158093181487	37.0	35.0	37.0	35.0	37.0
8	35.59035365090791	37.0	35.0	37.0	33.0	37.0
9	37.391299690651856	39.0	37.0	39.0	34.0	39.0
10	36.80285697147313	39.0	37.0	39.0	32.0	39.0
11	36.88307325809575	39.0	37.0	39.0	33.0	39.0
12	36.852416943023385	39.0	35.0	39.0	33.0	39.0
13	36.72656537871582	39.0	35.0	39.0	33.0	39.0
14	37.731861144673815	40.0	36.0	41.0	33.0	41.0
15	37.832240760259474	40.0	37.0	41.0	33.0	41.0
16	37.82484554015956	40.0	36.0	41.0	33.0	41.0
17	37.76788177930881	39.0	36.0	41.0	33.0	41.0
18	37.69482934437046	39.0	36.0	41.0	33.0	41.0
19	37.58860981859002	39.0	36.0	41.0	33.0	41.0
20	37.573887931994825	39.0	36.0	41.0	33.0	41.0
21	37.56246518762265	39.0	36.0	41.0	33.0	41.0
22	37.686033059975834	39.0	36.0	41.0	33.0	41.0
23	37.66818769976949	39.0	36.0	41.0	33.0	41.0
24	37.629532035956366	39.0	35.0	41.0	33.0	41.0
25	37.355304763618605	39.0	35.0	41.0	32.0	41.0
26	37.42725605628251	39.0	35.0	41.0	33.0	41.0
27	37.423665561239794	39.0	35.0	41.0	33.0	41.0
28	37.29241540056728	39.0	35.0	41.0	32.0	41.0
29	37.24965937427697	39.0	35.0	41.0	32.0	41.0
30	37.11999023111134	39.0	35.0	41.0	32.0	41.0
31	37.083125530219284	39.0	35.0	41.0	32.0	41.0
32	37.04935002613606	39.0	35.0	41.0	32.0	41.0
33	36.97442522087115	39.0	35.0	41.0	31.0	41.0
34	36.88601249389445	39.0	35.0	41.0	31.0	41.0
35	36.88055819772573	39.0	35.0	41.0	31.0	41.0
36	36.84015013239415	39.0	35.0	41.0	31.0	41.0
37	36.77816910460423	39.0	35.0	41.0	31.0	41.0
38	36.66188505274343	39.0	35.0	41.0	31.0	41.0
39	36.63232131074492	39.0	35.0	41.0	31.0	41.0
40	36.49389444458726	39.0	35.0	41.0	31.0	41.0
41	36.324425649331175	39.0	35.0	41.0	30.0	41.0
42	36.428472882764765	39.0	35.0	41.0	31.0	41.0
43	36.394504571668506	39.0	35.0	41.0	31.0	41.0
44	36.27481854717773	39.0	35.0	41.0	30.0	41.0
45	36.22878908626614	39.0	35.0	40.0	30.0	41.0
46	36.09179327660522	38.0	35.0	40.0	30.0	41.0
47	35.980963520913136	38.0	35.0	40.0	30.0	41.0
48	35.93463842258156	38.0	35.0	40.0	29.0	41.0
49	35.85722854914865	38.0	35.0	40.0	29.0	41.0
50	35.6930383814494	38.0	35.0	40.0	29.0	41.0
51	34.53099908309554	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	8.0
11	7.0
12	9.0
13	8.0
14	10.0
15	20.0
16	41.0
17	55.0
18	88.0
19	159.0
20	280.0
21	461.0
22	670.0
23	908.0
24	1204.0
25	1629.0
26	1982.0
27	2370.0
28	2668.0
29	3173.0
30	4054.0
31	4872.0
32	6575.0
33	9297.0
34	15922.0
35	20168.0
36	19077.0
37	27393.0
38	47636.0
39	62618.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.643418425495085	20.864289570425974	22.24564470380558	11.246647300273358
2	31.512806670265732	29.612157981781877	22.723377636100327	16.15165771185206
3	30.8058476224753	28.671259758177158	24.69557914942115	15.827313469926391
4	26.930855120525806	25.492514803294004	29.391501066865473	18.18512900931472
5	28.310067953760594	29.389787226749615	22.959459112059434	19.340685707430357
6	24.049461425743594	38.08409813448503	24.39437174905953	13.472068690711843
7	74.5953195026436	7.4089308208437235	13.308396959647634	4.6873527168650435
8	74.11073120988543	10.88545549585679	9.507528042708897	5.496285251548883
9	68.06173252097312	9.923991190861804	11.359760747919827	10.654515540245251
10	37.09864006786807	29.73855369032623	18.233544992587642	14.92926124921806
11	31.649485419505215	26.12663564616057	24.09787740901651	18.126001525317704
12	27.46643015673068	23.090139420893426	29.1040043874307	20.3394260349452
13	25.496370943554673	25.92183175231583	30.939955611541	17.641841692588496
14	19.454227615105786	32.49955011696959	27.614248866723223	20.431973401201404
15	18.267393334875788	27.68880091176294	35.05531418973924	18.988491563622027
16	20.06863929664002	26.487398990548172	30.638319751150416	22.805641961661397
17	19.636751587444408	26.903433678672116	28.623700694962167	24.83611403892131
18	21.356590143705496	27.42272723377636	28.60527691371672	22.615405708801426
19	23.768391646743275	28.514871847605338	25.535789266219354	22.180947239432033
20	24.8065502969228	26.56109411552996	29.05687378424467	19.57548180330257
21	24.37466258772719	29.230400095975046	27.410301892936406	18.984635423361354
22	20.18775118469198	26.711055125667325	27.7723506174109	25.32884307222979
23	21.21348449403155	29.780971233193654	27.361885909663485	21.643658363111307
24	22.781219740010457	26.586373257238833	26.982270324001473	23.650136678749238
25	22.024987788889174	30.190579020883142	25.360120654344154	22.42431253588353
26	21.30688878034568	27.858471083232644	26.43426994695665	24.400370189465026
27	23.37463688012545	28.431750601986337	26.23889217374911	21.954720344139094
28	19.810277899174785	28.654121357018603	29.20897709452685	22.32662364927976
29	23.01601583588267	27.94244924890957	26.172052409230744	22.869482505977018
30	21.858745297651183	28.42618062160981	26.4004216046685	23.314652476070506
31	23.042580357678432	30.782282320882288	23.602149155505284	22.572988165934
32	24.423507031029075	32.39029280958379	22.74222987737474	20.44397028201239
33	23.462899646092016	28.64383831632347	23.535309390986914	24.3579526465976
34	22.976597513217992	30.604899868891234	24.358809566655527	22.05969305123525
35	21.755914890699845	28.42489524152292	24.50877057679289	25.310419290984342
36	22.793216620821443	30.575336126892722	23.59872147527357	23.03272577701226
37	19.242139900768656	32.38172360900452	25.827570545943768	22.54856594428306
38	21.602097740301808	31.65891154014242	23.62485753704037	23.114133182515403
39	22.794073540879374	29.36707884521453	25.070053214735598	22.768794399170503
40	23.360497699169645	27.330608327549122	28.139112402204	21.169781571077234
41	18.858239714817003	29.90094004130355	25.489944043120218	25.750876200759233
42	20.808589766660667	29.797681174323248	26.63907384080139	22.754655218214694
43	21.884881359417978	26.878154536963244	25.348123773533164	25.888840330085607
44	22.119677455290194	26.60394011842635	27.19221573819378	24.084166688089667
45	21.261043557246545	27.17636271712212	24.758991233707807	26.803602491923527
46	22.639399470423406	29.70470534803808	25.143748339717387	22.512146841821128
47	20.36470517665407	27.18621729778829	29.90565310162215	22.54342442393549
48	20.466678663547476	27.92616776780894	26.692202884392913	24.914950684250666
49	20.73403772162095	26.06750816216355	29.388501846662724	23.809952269552774
50	20.07463773704551	25.679751835951226	28.30963949373163	25.935970933271633
51	19.52278121974001	25.82542824579895	26.808315552242128	27.843474982218908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1989.0
1	1489.5
2	990.0
3	1318.0
4	1646.0
5	1136.0
6	626.0
7	592.0
8	558.0
9	576.0
10	594.0
11	585.5
12	577.0
13	566.0
14	555.0
15	591.5
16	628.0
17	561.0
18	494.0
19	559.0
20	624.0
21	693.5
22	763.0
23	840.0
24	917.0
25	1186.5
26	1666.5
27	1877.0
28	2262.5
29	2648.0
30	3007.0
31	3366.0
32	4119.5
33	4873.0
34	5673.5
35	6474.0
36	6797.5
37	7121.0
38	7140.5
39	7160.0
40	8403.0
41	9646.0
42	11021.0
43	12396.0
44	13738.0
45	15080.0
46	21591.5
47	28103.0
48	25778.0
49	23453.0
50	22707.5
51	21962.0
52	19074.5
53	16187.0
54	14143.5
55	12100.0
56	11199.5
57	10299.0
58	10165.5
59	10032.0
60	9059.0
61	8086.0
62	7414.5
63	6743.0
64	5828.5
65	4914.0
66	3858.5
67	2803.0
68	2528.5
69	2254.0
70	1768.5
71	1283.0
72	1170.5
73	1058.0
74	834.0
75	405.5
76	201.0
77	156.0
78	111.0
79	95.0
80	79.0
81	58.0
82	37.0
83	24.5
84	12.0
85	8.5
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	233394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.63922808641182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8549449972433	24.432076231608356
2	9.481740134947097	6.18953357841247
3	3.3421722807109666	3.2725777012262527
4	1.6107012523300692	2.102881822154811
5	1.1171204284701621	1.823097423241386
6	0.8217595631284623	1.6092958687884007
7	0.677360917850298	1.5475976246175993
8	0.5880963007692509	1.5356007438066102
9	0.5027698285594265	1.4769017198385561
>10	6.851059360970359	45.263374379804105
>50	0.09320276195226967	2.005621395580006
>100	0.04725773845467195	3.2541539199808054
>500	0.005250859828296883	1.1936896406934197
>1k	0.006563574785371105	4.2935979502472215
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC	2683	1.1495582577101382	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG	2290	0.9811734663273264	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC	2233	0.9567512446763842	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1797	0.7699426720481246	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	1018	0.43617230948524816	Illumina Single End Adapter 1 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	937	0.4014670471391724	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	657	0.28149823902928095	Illumina Single End Adapter 1 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	596	0.2553621772624832	Illumina Single End Adapter 1 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTT	596	0.2553621772624832	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT	488	0.2090884941343822	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	457	0.19580623323650134	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	431	0.18466627248344003	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT	415	0.17781091202001767	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	413	0.17695399196208986	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA	392	0.167956331353848	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC	380	0.16281481100628123	No Hit
AAAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC	304	0.130251848805025	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	246	0.1054011671251189	Illumina Single End Adapter 1 (95% over 24bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	243	0.10411578703822721	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2846002896389793E-4	0.0	0.0	0.2746428785658586	0.0
2	4.2846002896389793E-4	0.0	0.0	1.2468186842849431	0.0
3	4.2846002896389793E-4	0.0	0.0	1.7095555155659528	0.0
4	4.2846002896389793E-4	0.0	0.0	2.3993761621978287	0.0
5	4.2846002896389793E-4	0.0	0.0	4.562670848436549	0.0
6	8.569200579277959E-4	0.0	0.0	5.521992853286717	0.0
7	8.569200579277959E-4	0.0	0.0	6.343350728810509	0.0
8	8.569200579277959E-4	0.0	0.0	7.450062983624258	0.0
9	8.569200579277959E-4	0.0	0.0	7.791974086737448	0.0
10	8.569200579277959E-4	0.0	0.0	9.588506988183072	0.0
11	8.569200579277959E-4	0.0	0.0	10.750490586733164	0.0
12	8.569200579277959E-4	0.0	0.0	12.820380986657755	0.0
13	8.569200579277959E-4	0.0	0.0	13.30582619947385	0.0
14	8.569200579277959E-4	0.0	0.0	13.563330676881154	0.0
15	8.569200579277959E-4	0.0	0.0	14.039778229089007	0.0
16	8.569200579277959E-4	0.0	0.0	14.698749753635484	0.0
17	8.569200579277959E-4	0.0	0.0	15.410421861744517	0.0
18	8.569200579277959E-4	0.0	0.0	16.233065117355203	0.0
19	8.569200579277959E-4	0.0	0.0	16.87061364045348	0.0
20	0.001285380086891694	0.0	0.0	17.392906415760475	0.0
21	0.001285380086891694	0.0	0.0	17.9409067928053	0.0
22	0.001285380086891694	0.0	0.0	18.55317617419471	0.0
23	0.001285380086891694	0.0	0.0	19.126455692948404	0.0
24	0.001285380086891694	0.0	0.0	19.603760165214187	0.0
25	0.001285380086891694	0.0	0.0	20.049358595336642	0.0
26	0.0017138401158555917	0.0	0.0	20.49881316571977	0.0
27	0.0017138401158555917	0.0	0.0	20.90884941343822	0.0
28	0.0017138401158555917	0.0	0.0	21.359589363908242	0.0
29	0.00214230014481949	0.0	0.0	21.768768691568763	0.0
30	0.00214230014481949	0.0	0.0	22.243502403660763	0.0
31	0.00214230014481949	0.0	0.0	22.727662236389968	0.0
32	0.00214230014481949	0.0	0.0	23.161692245730396	0.0
33	0.00214230014481949	0.0	0.0	23.600863775418393	0.0
34	0.00214230014481949	0.0	0.0	24.034893784758818	0.0
35	0.00214230014481949	0.0	0.0	24.509199036821855	0.0
36	0.00214230014481949	0.0	0.0	24.958653607204983	0.0
37	0.00214230014481949	0.0	0.0	25.400824357095726	0.0
38	0.00214230014481949	0.0	0.0	25.82328594565413	0.0
39	0.00214230014481949	0.0	0.0	26.282166636674464	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTT	20	7.02172E-4	45.000004	38
AAATCGT	20	7.02172E-4	45.000004	12
AGATCCC	20	7.02172E-4	45.000004	10
CGAAAAC	20	7.02172E-4	45.000004	19
GCGCGTG	20	7.02172E-4	45.000004	1
ATGTGCG	20	7.02172E-4	45.000004	1
CCAATTA	20	7.02172E-4	45.000004	30
CACGCCA	40	6.7757355E-9	45.000004	27
TGTATCG	20	7.02172E-4	45.000004	1
GACCCCT	20	7.02172E-4	45.000004	21
ATCGTTG	20	7.02172E-4	45.000004	23
ATCGTAA	20	7.02172E-4	45.000004	14
ACAGGCG	40	6.7757355E-9	45.000004	33
CGTAAGG	40	6.7757355E-9	45.000004	2
ACCGTTC	20	7.02172E-4	45.000004	39
ACGTAGG	20	7.02172E-4	45.000004	2
CGCACGG	20	7.02172E-4	45.000004	2
GTCTTAC	45	3.8198777E-10	45.000004	31
TATACGG	20	7.02172E-4	45.000004	2
CGTTTTC	20	7.02172E-4	45.000004	1
>>END_MODULE