SRR3550730_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316362
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	2118	0.669486221480456	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	1939	0.6129054690512767	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	1929	0.6097445331613784	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1267	0.4004905772501123	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1181	0.373306528596987	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1021	0.3227315543586145	RNA PCR Primer, Index 24 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	882	0.2787945454890284	RNA PCR Primer, Index 3 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTT	713	0.22537472894974744	No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	681	0.21525973410207294	TruSeq Adapter, Index 3 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	591	0.18681131109298843	TruSeq Adapter, Index 3 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGCATGT	461	0.14571914452431076	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	429	0.13560414967663625	TruSeq Adapter, Index 3 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	365	0.11537415998128725	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	324	0.1024143228327043	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTA	320	0.10114994847674501	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.025527E-4	45.000004	2
CGTGCGG	20	7.025527E-4	45.000004	2
TTAGCGG	25	3.8843747E-5	45.000004	2
GCTATGC	20	7.025527E-4	45.000004	1
GTTACGG	20	7.025527E-4	45.000004	2
CGAGGGT	25	3.8843747E-5	45.000004	4
GGTATAC	20	7.025527E-4	45.000004	8
CGAATAT	40	6.7884685E-9	45.000004	14
AGCGAAG	25	3.8843747E-5	45.000004	1
GCGATCT	40	6.7884685E-9	45.000004	9
ATGACCG	20	7.025527E-4	45.000004	12
CACACGG	20	7.025527E-4	45.000004	2
GCGTATG	20	7.025527E-4	45.000004	1
GTACGAG	20	7.025527E-4	45.000004	1
CTCGCAG	45	3.8380676E-10	45.0	30
TCACTCG	45	3.8380676E-10	45.0	27
CTCACGA	30	2.1604974E-6	44.999996	24
CGTATGG	30	2.1604974E-6	44.999996	2
CGGTCTA	30	2.1604974E-6	44.999996	31
CACTCGC	50	1.0768417E-9	40.500004	28