##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316362
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84053078435463	33.0	31.0	34.0	30.0	34.0
2	31.897285388257757	33.0	31.0	34.0	30.0	34.0
3	31.967480291564726	33.0	31.0	34.0	30.0	34.0
4	35.704591575473664	37.0	35.0	37.0	33.0	37.0
5	35.73926072031407	37.0	35.0	37.0	35.0	37.0
6	35.692766514309554	37.0	35.0	37.0	33.0	37.0
7	35.95133107010324	37.0	35.0	37.0	35.0	37.0
8	35.74993203987837	37.0	35.0	37.0	35.0	37.0
9	37.55873651070609	39.0	37.0	39.0	35.0	39.0
10	36.79084719403721	39.0	37.0	39.0	32.0	39.0
11	36.89943482466288	39.0	37.0	39.0	33.0	39.0
12	36.919614239383996	39.0	37.0	39.0	33.0	39.0
13	36.87768758574039	39.0	37.0	39.0	33.0	39.0
14	37.9546595355953	40.0	37.0	41.0	33.0	41.0
15	38.06435665471833	40.0	37.0	41.0	33.0	41.0
16	38.01735669897143	40.0	37.0	41.0	33.0	41.0
17	37.98229243714479	40.0	37.0	41.0	33.0	41.0
18	37.88281778468969	39.0	37.0	41.0	33.0	41.0
19	37.76807581188638	39.0	36.0	41.0	33.0	41.0
20	37.83140832337639	39.0	36.0	41.0	33.0	41.0
21	37.78807821419766	39.0	36.0	41.0	33.0	41.0
22	37.89001839664688	40.0	36.0	41.0	33.0	41.0
23	37.88895632218787	40.0	36.0	41.0	33.0	41.0
24	37.853250390375585	40.0	36.0	41.0	33.0	41.0
25	37.624414436626395	39.0	36.0	41.0	33.0	41.0
26	37.670826458297775	39.0	36.0	41.0	33.0	41.0
27	37.70514157831851	40.0	36.0	41.0	33.0	41.0
28	37.56668942540507	40.0	36.0	41.0	33.0	41.0
29	37.54747409613038	40.0	36.0	41.0	33.0	41.0
30	37.43564334528167	39.0	36.0	41.0	33.0	41.0
31	37.47451969579153	40.0	36.0	41.0	33.0	41.0
32	37.44214539040719	40.0	36.0	41.0	33.0	41.0
33	37.37074617052617	40.0	36.0	41.0	33.0	41.0
34	37.33023877709712	40.0	36.0	41.0	33.0	41.0
35	37.31269874384408	40.0	36.0	41.0	33.0	41.0
36	37.236340015551804	40.0	36.0	41.0	32.0	41.0
37	37.17932305396982	40.0	35.0	41.0	32.0	41.0
38	37.107342221885055	40.0	35.0	41.0	32.0	41.0
39	37.063775042514585	40.0	35.0	41.0	32.0	41.0
40	36.99141805905893	39.0	35.0	41.0	32.0	41.0
41	36.85860817670896	39.0	35.0	41.0	31.0	41.0
42	36.92180160701981	39.0	35.0	41.0	32.0	41.0
43	36.85843116429913	39.0	35.0	41.0	31.0	41.0
44	36.73698484647334	39.0	35.0	41.0	31.0	41.0
45	36.731990567767305	39.0	35.0	41.0	31.0	41.0
46	36.62150637560769	39.0	35.0	41.0	31.0	41.0
47	36.57774954008383	39.0	35.0	41.0	31.0	41.0
48	36.483657961449225	39.0	35.0	41.0	31.0	41.0
49	36.40624031963384	39.0	35.0	41.0	31.0	41.0
50	36.283219855734885	39.0	35.0	41.0	30.0	41.0
51	35.102063458948926	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	8.0
11	5.0
12	3.0
13	7.0
14	7.0
15	14.0
16	24.0
17	51.0
18	97.0
19	144.0
20	329.0
21	508.0
22	729.0
23	995.0
24	1369.0
25	1955.0
26	2516.0
27	2947.0
28	3349.0
29	3728.0
30	4546.0
31	5907.0
32	7731.0
33	11040.0
34	19790.0
35	24479.0
36	23694.0
37	35335.0
38	67140.0
39	97875.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.655173503770996	20.7053944531897	22.97368204778071	11.665749995258597
2	32.39390318685557	27.039593882956865	24.34584431758555	16.22065861260202
3	30.991395932507697	26.66470688641493	26.69410359019098	15.649793590886391
4	27.56494142785796	25.992691916222554	28.41491708865161	18.027449567267876
5	26.638155024939785	29.912252419696422	25.435418918833484	18.014173636530302
6	24.91797371365714	37.41694640949292	24.375241021361603	13.289838855488334
7	76.63372971469393	7.126013870186684	11.734658397658379	4.5055980174610095
8	77.38919339237962	8.82786175330792	9.024155872070603	4.758788982241862
9	71.63692225994272	8.794988020052976	10.858446968978575	8.709642751025724
10	37.39102673519576	28.00209886143089	19.967632016487443	14.63924238688591
11	31.1592416282613	26.746891219552282	24.14733754369994	17.94652960848648
12	26.573671932785857	24.05156118623602	29.58730821021488	19.78745867076324
13	24.777628160145657	25.944329597107114	31.253121424191278	18.02492081855596
14	20.27993248240939	30.327599395629058	29.344232240281702	20.048235881679847
15	19.54501488800804	27.58106220089644	34.24368286962404	18.63024004147148
16	22.480576048956575	26.696316245313913	30.557715528413652	20.26539217731586
17	22.739456698339243	26.2989866039537	28.142760508531367	22.818796189175693
18	22.441064350332844	27.311750463077107	29.348973644116548	20.898211542473497
19	23.82018067909547	28.24517483136407	27.467268508860105	20.467375980680362
20	24.335729322737876	27.016519050960607	29.006012100062588	19.64173952623893
21	23.884031584071412	28.579601848515306	29.009805223130464	18.526561344282815
22	21.503846858978008	26.7930408835448	28.53819358835764	23.16491866911955
23	21.6220658612602	28.68486101364892	28.81825250820263	20.874820616888247
24	21.97040099632699	26.93970830883608	28.610262926647323	22.479627768189605
25	21.363817399055513	29.931218035035812	27.38413589495578	21.320828670952896
26	20.46800816785834	28.352014464442632	28.977879770642488	22.202097597056536
27	22.701525467660467	27.301319374640443	28.65040681244903	21.34674834525006
28	20.529014230533377	28.126323641903895	30.540646474608202	20.804015652954526
29	22.020975970565367	26.33502127309854	30.098431543611433	21.545571212724663
30	22.390173282505486	27.03516857271101	29.410295800380577	21.16436234440293
31	23.163654294763596	27.88198329761476	27.64270045074946	21.31166195687219
32	24.230786251193255	28.18100783279914	28.088392411225115	19.499813504782495
33	23.573311586094412	27.267813454207523	26.4668322997073	22.69204265999077
34	21.554737926805366	27.67494199682642	29.12928859976862	21.641031476599593
35	22.43853560162093	26.837926173181355	28.842591714554842	21.88094651064287
36	23.17376928961127	27.16381866342987	28.965868214260876	20.696543832697987
37	22.816267440463772	28.189542359701857	29.284806645551615	19.709383554282752
38	21.492783583363362	28.09439818941592	27.891150011695466	22.521668215525253
39	23.602076102692486	26.762695899001777	27.678419026305306	21.95680897200043
40	22.423047015760424	26.27844052066936	29.69003862663657	21.60847383693364
41	20.840682509277347	27.296894064394582	28.349801809319704	23.512621617008364
42	21.97387802580588	26.267377245054718	30.422427472326007	21.3363172568134
43	22.96862455035687	25.87447291394036	28.591613404896922	22.565289130805848
44	22.511237127088588	26.484217447101738	28.536297026823704	22.46824839898597
45	21.968188341204065	25.329527566521897	27.390141673146584	25.312142419127454
46	22.574139751297565	28.050145086957347	27.43692352431708	21.93879163742801
47	21.764307976305624	25.798926546171792	31.07642510794596	21.360340369576626
48	21.20134529431474	26.345452361535205	29.340439117213823	23.11276322693623
49	22.170488238157553	24.340786820161714	30.63800330001707	22.85072164166366
50	20.704130078833742	24.74949583072556	30.119293720484762	24.427080369955938
51	20.85206187848098	24.867714833007756	28.683280545703973	25.59694274280729
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1381.0
1	1184.0
2	987.0
3	1620.0
4	2253.0
5	1542.0
6	831.0
7	790.5
8	750.0
9	794.5
10	839.0
11	894.0
12	949.0
13	879.5
14	810.0
15	852.5
16	895.0
17	840.5
18	786.0
19	871.0
20	956.0
21	986.5
22	1017.0
23	1257.5
24	1498.0
25	1717.0
26	2302.0
27	2668.0
28	3381.5
29	4095.0
30	4751.5
31	5408.0
32	6356.5
33	7305.0
34	7795.5
35	8286.0
36	9377.0
37	10468.0
38	11338.5
39	12209.0
40	13549.5
41	14890.0
42	17598.0
43	20306.0
44	21964.0
45	23622.0
46	29499.0
47	35376.0
48	33401.0
49	31426.0
50	30534.0
51	29642.0
52	25487.5
53	21333.0
54	18956.0
55	16579.0
56	15072.5
57	13566.0
58	12883.5
59	12201.0
60	11249.0
61	10297.0
62	9196.5
63	8096.0
64	6490.0
65	4884.0
66	3974.5
67	3065.0
68	2490.5
69	1916.0
70	1578.0
71	1240.0
72	997.5
73	755.0
74	572.0
75	308.5
76	228.0
77	183.5
78	139.0
79	83.0
80	27.0
81	34.5
82	42.0
83	25.5
84	9.0
85	5.5
86	2.0
87	3.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	316362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.38032934874884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9491129669059	23.621163035674975
2	9.525103718782534	6.168519909903416
3	3.5457454225397758	3.4443721370596965
4	1.9066104458342872	2.469466967035163
5	1.28498778537002	2.080416384970032
6	1.0131965291935938	1.968458238617867
7	0.8443300413737029	1.9137779373106265
8	0.802363307042576	2.0784630511511915
9	0.6255036466968276	1.8228612679998035
>10	7.383141283903741	46.24709688109618
>50	0.0629500474874337	1.4061333098612931
>100	0.04696273383983149	2.792530280122601
>500	0.003996828411900552	0.9276079611993391
>1k	0.005995242617850828	3.0591326379978208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	2118	0.669486221480456	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	1939	0.6129054690512767	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	1929	0.6097445331613784	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1267	0.4004905772501123	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1181	0.373306528596987	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1021	0.3227315543586145	RNA PCR Primer, Index 24 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	882	0.2787945454890284	RNA PCR Primer, Index 3 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTT	713	0.22537472894974744	No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	681	0.21525973410207294	TruSeq Adapter, Index 3 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	591	0.18681131109298843	TruSeq Adapter, Index 3 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGCATGT	461	0.14571914452431076	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	429	0.13560414967663625	TruSeq Adapter, Index 3 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	365	0.11537415998128725	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	324	0.1024143228327043	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTA	320	0.10114994847674501	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22758738407267623	0.0
2	0.0	0.0	0.0	1.0282524449839108	0.0
3	0.0	0.0	0.0	1.4486569183403821	0.0
4	0.0	0.0	0.0	1.9702113401735986	0.0
5	0.0	0.0	0.0	3.547518349232841	0.0
6	0.0	0.0	0.0	4.4218332163787055	0.0
7	0.0	0.0	0.0	5.102066619884815	0.0
8	0.0	0.0	0.0	6.036123175349758	0.0
9	0.0	0.0	0.0	6.337676459246054	0.0
10	0.0	0.0	0.0	7.717741068775643	0.0
11	0.0	0.0	0.0	8.728608366365114	0.0
12	0.0	0.0	0.0	10.123845468166214	0.0
13	0.0	0.0	0.0	10.530974010785114	0.0
14	0.0	0.0	0.0	10.74054406028537	0.0
15	0.0	0.0	0.0	11.098362003021855	0.0
16	0.0	0.0	0.0	11.63824985301648	0.0
17	0.0	0.0	0.0	12.347563866709656	0.0
18	0.0	0.0	0.0	13.123573627679683	0.0
19	0.0	0.0	0.0	13.7165652006246	0.0
20	0.0	0.0	0.0	14.198291830245099	0.0
21	0.0	0.0	0.0	14.714156567476499	0.0
22	0.0	0.0	0.0	15.339389686498379	0.0
23	0.0	0.0	0.0	15.897610964654415	0.0
24	0.0	0.0	0.0	16.358159323812593	0.0
25	0.0	0.0	0.0	16.799742067631385	0.0
26	0.0	0.0	0.0	17.22962934865755	0.0
27	0.0	0.0	0.0	17.66773506299745	0.0
28	0.0	0.0	0.0	18.09793843761261	0.0
29	0.0	0.0	0.0	18.51739463020211	0.0
30	0.0	0.0	0.0	19.021563904640885	0.0
31	0.0	0.0	0.0	19.48811804198987	0.0
32	0.0	0.0	0.0	19.942344529368256	0.0
33	0.0	0.0	0.0	20.40921476030623	0.0
34	0.0	0.0	0.0	20.843843445167245	0.0
35	0.0	0.0	0.0	21.317983828651986	0.0
36	0.0	0.0	0.0	21.745026267377245	0.0
37	3.160935889898281E-4	0.0	0.0	22.21442524702714	0.0
38	3.160935889898281E-4	0.0	0.0	22.682559852321074	0.0
39	6.321871779796562E-4	0.0	0.0	23.14690133454713	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.025527E-4	45.000004	2
CGTGCGG	20	7.025527E-4	45.000004	2
TTAGCGG	25	3.8843747E-5	45.000004	2
GCTATGC	20	7.025527E-4	45.000004	1
GTTACGG	20	7.025527E-4	45.000004	2
CGAGGGT	25	3.8843747E-5	45.000004	4
GGTATAC	20	7.025527E-4	45.000004	8
CGAATAT	40	6.7884685E-9	45.000004	14
AGCGAAG	25	3.8843747E-5	45.000004	1
GCGATCT	40	6.7884685E-9	45.000004	9
ATGACCG	20	7.025527E-4	45.000004	12
CACACGG	20	7.025527E-4	45.000004	2
GCGTATG	20	7.025527E-4	45.000004	1
GTACGAG	20	7.025527E-4	45.000004	1
CTCGCAG	45	3.8380676E-10	45.0	30
TCACTCG	45	3.8380676E-10	45.0	27
CTCACGA	30	2.1604974E-6	44.999996	24
CGTATGG	30	2.1604974E-6	44.999996	2
CGGTCTA	30	2.1604974E-6	44.999996	31
CACTCGC	50	1.0768417E-9	40.500004	28
>>END_MODULE