SRR3550727_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550727_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374426
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	5148	1.3749045205194084	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	4663	1.2453729174790211	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	4372	1.167653955654789	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	3712	0.991384145331788	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2191	0.5851623551783263	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	1716	0.4583015068398028	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTT	1590	0.42464999759632077	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1491	0.39820952604787063	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	1288	0.34399320560003843	TruSeq Adapter, Index 27 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1278	0.3413224508981748	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	995	0.26574009283543343	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	897	0.23956669675716963	No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	826	0.2206043383739377	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	775	0.2069834893944331	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTTGCT	708	0.1890894328919466	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA	666	0.17787226314411927	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	484	0.1292645275702008	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	458	0.12232056534535529	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCC	443	0.1183144332925598	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	429	0.1145753767099507	TruSeq Adapter, Index 27 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	407	0.10869971636585066	TruSeq Adapter, Index 27 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGC	20	7.027186E-4	45.000004	6
CGAACAG	20	7.027186E-4	45.000004	12
GCGAACA	20	7.027186E-4	45.000004	33
ACCGAAC	20	7.027186E-4	45.000004	10
GCCGATT	20	7.027186E-4	45.000004	9
TACCTGC	20	7.027186E-4	45.000004	37
ATCCACG	20	7.027186E-4	45.000004	16
GATTCTA	30	2.161567E-6	45.000004	9
AGGGCGT	20	7.027186E-4	45.000004	6
GTACGAG	20	7.027186E-4	45.000004	1
CGCCACG	20	7.027186E-4	45.000004	42
ACTATGC	175	0.0	45.0	8
CCCTCGT	170	0.0	45.0	14
TAGGTGA	45	3.8380676E-10	45.0	28
ATTCCTC	50	2.1827873E-11	45.0	11
CCATAGG	25	3.88575E-5	45.0	2
AACTATG	175	0.0	45.0	7
GCCCTCG	175	0.0	43.714287	13
TCGTGTA	170	0.0	43.676468	17
CCTCGTG	180	0.0	42.5	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content