##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550727_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374426
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47853247370642	31.0	31.0	34.0	30.0	34.0
2	31.52704139135637	31.0	31.0	34.0	30.0	34.0
3	31.64470950201108	31.0	31.0	34.0	30.0	34.0
4	35.44135289750177	37.0	35.0	37.0	33.0	37.0
5	35.46563005774172	37.0	35.0	37.0	33.0	37.0
6	35.41751107027824	37.0	35.0	37.0	33.0	37.0
7	35.70401361016596	37.0	35.0	37.0	35.0	37.0
8	35.45269292196589	37.0	35.0	37.0	33.0	37.0
9	37.21962417139835	39.0	37.0	39.0	34.0	39.0
10	36.67934919049425	39.0	35.0	39.0	32.0	39.0
11	36.711945751630495	39.0	35.0	39.0	32.0	39.0
12	36.783612783300306	39.0	35.0	39.0	33.0	39.0
13	36.619099635174905	39.0	35.0	39.0	32.0	39.0
14	37.56842206470704	39.0	36.0	41.0	33.0	41.0
15	37.678692184837594	39.0	36.0	41.0	33.0	41.0
16	37.65439900006944	39.0	36.0	41.0	33.0	41.0
17	37.621548717236514	39.0	36.0	41.0	33.0	41.0
18	37.63004972945255	39.0	36.0	41.0	33.0	41.0
19	37.50742736882588	39.0	36.0	41.0	32.0	41.0
20	37.51881012536523	39.0	36.0	41.0	32.0	41.0
21	37.48427459631543	39.0	36.0	40.0	32.0	41.0
22	37.58955841741759	39.0	36.0	41.0	33.0	41.0
23	37.60437843525823	39.0	36.0	41.0	33.0	41.0
24	37.6177963068804	39.0	36.0	41.0	33.0	41.0
25	37.33208163962973	39.0	35.0	41.0	32.0	41.0
26	37.45226560121359	39.0	36.0	41.0	33.0	41.0
27	37.4455406408743	39.0	36.0	41.0	33.0	41.0
28	37.27149556921795	39.0	36.0	41.0	32.0	41.0
29	37.25460037497396	39.0	36.0	41.0	32.0	41.0
30	37.185964649890764	39.0	36.0	41.0	32.0	41.0
31	37.21198581295102	39.0	36.0	41.0	32.0	41.0
32	37.23766244865474	39.0	36.0	41.0	32.0	41.0
33	37.16789966508736	39.0	35.0	41.0	32.0	41.0
34	37.21057565446844	39.0	36.0	41.0	32.0	41.0
35	37.16655360471762	39.0	35.0	41.0	32.0	41.0
36	37.10243412583528	39.0	35.0	41.0	32.0	41.0
37	37.06296571285114	39.0	35.0	41.0	32.0	41.0
38	36.95971967758649	39.0	35.0	41.0	31.0	41.0
39	36.92118869950271	39.0	35.0	41.0	31.0	41.0
40	36.737494191108524	39.0	35.0	41.0	31.0	41.0
41	36.59691901737593	39.0	35.0	41.0	30.0	41.0
42	36.646739275584494	39.0	35.0	41.0	31.0	41.0
43	36.62403251910925	39.0	35.0	41.0	31.0	41.0
44	36.53341915358442	39.0	35.0	40.0	30.0	41.0
45	36.533269591321115	39.0	35.0	40.0	31.0	41.0
46	36.374509248823536	39.0	35.0	40.0	30.0	41.0
47	36.29954383509692	39.0	35.0	40.0	30.0	41.0
48	36.24213062127096	38.0	35.0	40.0	30.0	41.0
49	36.17810996031258	39.0	35.0	40.0	30.0	41.0
50	35.983887336883654	38.0	35.0	40.0	29.0	41.0
51	34.69247060834451	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	8.0
11	6.0
12	3.0
13	7.0
14	9.0
15	11.0
16	20.0
17	38.0
18	82.0
19	197.0
20	374.0
21	651.0
22	1002.0
23	1335.0
24	1817.0
25	2314.0
26	2909.0
27	3342.0
28	4117.0
29	4995.0
30	6113.0
31	8368.0
32	10928.0
33	14954.0
34	24322.0
35	32152.0
36	32403.0
37	46547.0
38	80482.0
39	94893.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.17702296314893	21.061571578896764	25.162515423608404	10.598890034345905
2	27.967074936035424	30.711275392200328	24.5690737288543	16.752575942909946
3	28.25444814195595	28.355135594216215	27.56459220246457	15.825824061363262
4	27.064626922275696	23.973495430338705	31.352256520647604	17.60962112673799
5	26.840550602789335	27.31941692083349	25.197502310202818	20.642530166174357
6	22.155779780250302	36.04824451293446	26.271946926762563	15.524028780052667
7	72.92469005891685	6.095730531533601	15.17709774428058	5.802481665268972
8	70.8572588442042	9.551420040274982	11.315987671796297	8.275333443724527
9	65.33787717733277	7.226528072302671	14.132565580381703	13.303029169982855
10	35.37494725259464	28.76536351642247	20.793427806829655	15.066261424153238
11	25.81978815573705	25.195365706441326	27.83140059718075	21.153445540640874
12	24.22294391949277	19.0179100810307	34.759605369285254	21.99954063019128
13	22.799965814339814	23.980706468033734	36.191129889484166	17.02819782814228
14	17.269901128660937	30.815434825573014	29.31473775859582	22.599926287170227
15	15.164278121711632	25.511850138612168	38.920908270259005	20.402963469417188
16	16.62544801910124	27.06168909210365	31.002654730173653	25.31020815862146
17	16.43128415227575	26.759893810793056	29.158765684006987	27.650056352924206
18	19.103908382430706	25.444547120125204	31.772633310720945	23.678911186723145
19	19.913147057095394	27.724570409106203	27.72430333363602	24.637979200162384
20	20.79663271247189	27.282293430477583	30.9703385982811	20.950735258769424
21	21.360696105505493	29.717754643107046	29.337706249031854	19.583843002355607
22	17.16761122357956	26.281027492748898	28.606186536191398	27.945174747480145
23	16.71705490537516	29.06982955243493	28.94777606255976	25.265339479630157
24	21.010026013150796	25.105361272988517	27.791072201182608	26.09354051267807
25	17.757580937221242	31.06969067319043	25.31047523409165	25.862253155496678
26	17.114196129542286	26.428186076821586	27.243033336360188	29.21458445727594
27	20.863401580018483	26.663212490585593	28.397600593975845	24.075785335420083
28	16.62972122662422	24.772318161666124	33.155550095345944	25.44241051636371
29	18.222559330815702	21.70201855640367	29.642706435984678	30.43271567679595
30	19.159460080229472	24.55438457799405	32.989429152890025	23.296726188886456
31	20.02371630175255	25.73592645809853	29.369488229984032	24.870869010164892
32	22.07672544107514	25.682778439531447	25.601053345654417	26.639442773739002
33	18.4047048014828	21.94211940410121	30.84187529712146	28.811300497294525
34	18.61863225310208	21.476606859566377	32.69698151303596	27.207779374295587
35	19.564079417561814	20.750695731599837	29.734580397728788	29.95064445310956
36	20.081671678782993	20.822539033079966	32.55649981571793	26.539289472419114
37	17.5703610326206	22.54303921202053	36.040499324299056	23.846100431059806
38	18.383071688397706	23.534156281882133	30.78952850496493	27.293243524755223
39	20.365839979061285	20.660157147206657	31.817234914242064	27.15676795948999
40	23.196038736626196	19.87896139691154	33.971198581295106	22.953801285167163
41	18.85686357250832	22.46077996720313	29.94690539652695	28.735451063761598
42	22.348875345195047	20.769658089983068	30.68376661877113	26.197699946050758
43	21.429601576813575	21.03673356016943	29.67261888864555	27.86104597437144
44	19.355226399876077	21.594387141918563	31.052063692158132	27.998322766047227
45	19.932109415478628	19.966562151132667	28.93655889281193	31.164769540576774
46	24.006078637701442	22.369173080929208	29.684103133863566	23.940645147505784
47	17.86254159700448	20.326846960414073	36.973393941660035	24.837217500921412
48	18.966898666225102	21.03246035264645	30.638630864309636	29.36201011681881
49	19.521614417802184	18.724127063825698	36.32253102081586	25.43172749755626
50	19.944661962577385	18.511000838616976	33.84139990278453	27.70293729602111
51	18.53450347999338	18.76418838435365	28.996650873603862	33.70465726204911
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	239.0
1	350.0
2	461.0
3	935.5
4	1410.0
5	959.5
6	509.0
7	506.0
8	503.0
9	590.5
10	678.0
11	718.5
12	759.0
13	830.5
14	902.0
15	911.5
16	921.0
17	895.0
18	869.0
19	927.5
20	986.0
21	1001.0
22	1016.0
23	1025.0
24	1034.0
25	1276.0
26	1973.5
27	2429.0
28	2639.5
29	2850.0
30	3507.0
31	4164.0
32	4883.5
33	5603.0
34	6965.5
35	8328.0
36	9038.5
37	9749.0
38	10394.0
39	11039.0
40	12803.5
41	14568.0
42	17331.5
43	20095.0
44	24330.5
45	28566.0
46	44708.0
47	60850.0
48	58699.5
49	56549.0
50	51686.5
51	46824.0
52	36802.5
53	26781.0
54	21537.0
55	16293.0
56	14512.5
57	12732.0
58	11339.5
59	9947.0
60	9424.5
61	8902.0
62	7529.0
63	6156.0
64	5174.0
65	4192.0
66	3324.0
67	2456.0
68	1903.0
69	1350.0
70	1123.0
71	896.0
72	777.5
73	659.0
74	479.5
75	237.0
76	174.0
77	122.5
78	71.0
79	67.0
80	63.0
81	42.0
82	21.0
83	14.5
84	8.0
85	5.0
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	374426.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.47341495013458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.04627807743165	27.007206258312177
2	11.44029244768679	8.345330671907423
3	4.193216416801185	4.588227670371184
4	1.9869653292256813	2.8988564377751618
5	1.181314088165678	2.154327946205332
6	0.8050685970517798	1.7618160602155355
7	0.6463494292770205	1.6502199655762404
8	0.5528823834575327	1.613240687237281
9	0.4493031332953745	1.474885765717004
>10	4.518472565083128	33.33484186601249
>50	0.10864863189216513	2.6612254299292455
>100	0.0594631025896309	3.8570230725556676
>500	0.004404674265898586	1.3031677399603216
>1k	0.0066070113988478795	5.971223293374797
>5k	7.341123776497643E-4	1.3784071348501614
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	5148	1.3749045205194084	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	4663	1.2453729174790211	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	4372	1.167653955654789	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	3712	0.991384145331788	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2191	0.5851623551783263	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	1716	0.4583015068398028	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTT	1590	0.42464999759632077	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1491	0.39820952604787063	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	1288	0.34399320560003843	TruSeq Adapter, Index 27 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1278	0.3413224508981748	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	995	0.26574009283543343	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	897	0.23956669675716963	No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	826	0.2206043383739377	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	775	0.2069834893944331	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTTGCT	708	0.1890894328919466	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA	666	0.17787226314411927	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	484	0.1292645275702008	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	458	0.12232056534535529	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCC	443	0.1183144332925598	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	429	0.1145753767099507	TruSeq Adapter, Index 27 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	407	0.10869971636585066	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.012264105590958E-4	0.0	0.0	0.49462377078514846	0.0
2	8.012264105590958E-4	0.0	0.0	2.7906715879773305	0.0
3	0.001068301880745461	0.0	0.0	3.652257054798545	0.0
4	0.001068301880745461	0.0	0.0	5.109954971075727	0.0
5	0.001068301880745461	0.0	0.0	9.228258721349478	0.0
6	0.001068301880745461	0.0	0.0	11.203548898847837	0.0
7	0.001068301880745461	0.0	0.0	13.047972095954876	0.0
8	0.001068301880745461	0.0	0.0	15.548332647839626	0.0
9	0.001068301880745461	0.0	0.0	16.365316511139717	0.0
10	0.001068301880745461	0.0	0.0	19.334127437731354	0.0
11	0.001068301880745461	0.0	0.0	22.101296384332283	0.0
12	0.001068301880745461	0.0	0.0	25.502502497155646	0.0
13	0.0013353773509318264	0.0	0.0	26.524867397029052	0.0
14	0.0013353773509318264	0.0	0.0	26.94177220598997	0.0
15	0.0013353773509318264	0.0	0.0	27.63536720206396	0.0
16	0.0013353773509318264	0.0	0.0	28.72129606384172	0.0
17	0.0013353773509318264	0.0	0.0	30.097803037182246	0.0
18	0.0013353773509318264	0.0	0.0	31.649511518965028	0.0
19	0.0013353773509318264	0.0	0.0	32.740781890146515	0.0
20	0.0013353773509318264	0.0	0.0	33.5716536778963	0.0
21	0.0013353773509318264	0.0	0.0	34.51069103107156	0.0
22	0.0013353773509318264	0.0	0.0	35.535459610176645	0.0
23	0.0013353773509318264	0.0	0.0	36.46595054830594	0.0
24	0.0013353773509318264	0.0	0.0	37.20735205354329	0.0
25	0.0013353773509318264	0.0	0.0	37.8280354462564	0.0
26	0.0013353773509318264	0.0	0.0	38.427886952294976	0.0
27	0.0013353773509318264	0.0	0.0	39.07420959014598	0.0
28	0.0013353773509318264	0.0	0.0	39.686613643283316	0.0
29	0.0013353773509318264	0.0	0.0	40.292340809665994	0.0
30	0.0013353773509318264	0.0	0.0	40.935992692815134	0.0
31	0.0013353773509318264	0.0	0.0	41.55293702894564	0.0
32	0.0013353773509318264	0.0	0.0	42.09510023342396	0.0
33	0.0013353773509318264	0.0	0.0	42.67545523013893	0.0
34	0.0013353773509318264	0.0	0.0	43.237649094881235	0.0
35	0.0016024528211181915	0.0	0.0	43.8708850346931	0.0
36	0.0016024528211181915	0.0	0.0	44.407706729767696	0.0
37	0.001869528291304557	0.0	0.0	44.927435594750364	0.0
38	0.002136603761490922	0.0	0.0	45.43167408246222	0.0
39	0.002136603761490922	0.0	0.0	46.00428389054179	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGC	20	7.027186E-4	45.000004	6
CGAACAG	20	7.027186E-4	45.000004	12
GCGAACA	20	7.027186E-4	45.000004	33
ACCGAAC	20	7.027186E-4	45.000004	10
GCCGATT	20	7.027186E-4	45.000004	9
TACCTGC	20	7.027186E-4	45.000004	37
ATCCACG	20	7.027186E-4	45.000004	16
GATTCTA	30	2.161567E-6	45.000004	9
AGGGCGT	20	7.027186E-4	45.000004	6
GTACGAG	20	7.027186E-4	45.000004	1
CGCCACG	20	7.027186E-4	45.000004	42
ACTATGC	175	0.0	45.0	8
CCCTCGT	170	0.0	45.0	14
TAGGTGA	45	3.8380676E-10	45.0	28
ATTCCTC	50	2.1827873E-11	45.0	11
CCATAGG	25	3.88575E-5	45.0	2
AACTATG	175	0.0	45.0	7
GCCCTCG	175	0.0	43.714287	13
TCGTGTA	170	0.0	43.676468	17
CCTCGTG	180	0.0	42.5	15
>>END_MODULE