SRR3550724_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550724_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475004
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4461	0.9391499861053803	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTC	4211	0.8865188503675758	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGC	2628	0.5532584988757989	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCG	2622	0.5519953516180917	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTT	1777	0.37410211282431305	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	1238	0.260629384173607	TruSeq Adapter, Index 22 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCT	1027	0.2162087056109001	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTG	768	0.16168284898653484	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTT	646	0.13599885474648635	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	636	0.13389360931697417	TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT	571	0.12020951402514504	TruSeq Adapter, Index 20 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	546	0.11494640045136462	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCT	536	0.11284115502185246	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTC	495	0.10420964876085254	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCT	479	0.10084125607363306	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGG	30	2.162802E-6	45.000004	2
TCACGAC	35	1.209919E-7	45.0	25
CGACGGT	40	6.8012014E-9	45.0	28
ATTAACG	20	7.0291024E-4	45.0	1
TCGATAG	20	7.0291024E-4	45.0	1
TCTACGG	20	7.0291024E-4	45.0	2
TGCGAAG	20	7.0291024E-4	45.0	1
GGTGCGT	20	7.0291024E-4	45.0	1
TATAGCG	20	7.0291024E-4	45.0	1
CGGTCTA	40	6.8012014E-9	45.0	31
TGTACCG	25	3.8873375E-5	45.0	1
GCCACGG	35	1.209919E-7	45.0	2
CGTTTTT	2635	0.0	43.37761	1
ACGGGTA	65	0.0	41.53846	5
CTAACGG	65	0.0	41.53846	2
ATTGCGG	65	0.0	41.53846	2
GCGATAT	60	3.6379788E-12	41.250004	9
CGAATAT	115	0.0	41.086956	14
ACGGTCT	50	1.0786607E-9	40.5	30
CGTGCGG	95	0.0	40.263157	2