FastQCFastQC Report
Sat 18 Jun 2016
SRR3550722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438989
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC56941.2970712250193057No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG55301.259712657948149No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC50251.1446756069058677No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC17220.3922649542471452No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11830.26948283442181925No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT10110.23030189822524028No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT9950.2266571599743957No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT8760.19954941923373934No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7980.18178132026087215No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTC7850.17881997043206094No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA7380.16811355182020504No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT7290.166063386554105No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC6150.14009462651683754No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT6030.13736107282870413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA650.045.00000431
CTCGTAT207.0285174E-445.043
CGCGGGT207.0285174E-445.04
GGGTACG502.1827873E-1145.07
TACGGTT253.8868522E-545.033
TACGGAT207.0285174E-445.018
TAACGCC253.8868522E-545.012
TCTCGTA253.8868522E-545.028
CGGGCTA253.8868522E-545.06
GACGGGC406.7993824E-945.04
TCGTACG253.8868522E-545.030
ATAACGC253.8868522E-545.011
TCGAGAG207.0285174E-445.01
AGGGCGC253.8868522E-545.06
TCGAAAG207.0285174E-445.01
TACGGCT6650.042.9699257
ACGGCTG7050.040.5319188
CATGCGG451.9235813E-840.02
TATACTC451.9235813E-840.043
CGGCTGT7250.039.7241369