##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550722_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438989
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80337776117397	33.0	31.0	34.0	30.0	34.0
2	31.84659524498336	33.0	31.0	34.0	30.0	34.0
3	31.93464073131673	33.0	31.0	34.0	30.0	34.0
4	35.676741330648376	37.0	35.0	37.0	33.0	37.0
5	35.68343398126149	37.0	35.0	37.0	35.0	37.0
6	35.625225233434094	37.0	35.0	37.0	33.0	37.0
7	35.887031337915076	37.0	35.0	37.0	35.0	37.0
8	35.632633163928936	37.0	35.0	37.0	33.0	37.0
9	37.45990901822141	39.0	37.0	39.0	35.0	39.0
10	36.904717430277294	39.0	37.0	39.0	32.0	39.0
11	36.8879812478217	39.0	37.0	39.0	33.0	39.0
12	36.89387433398103	39.0	37.0	39.0	33.0	39.0
13	36.79373515053908	39.0	35.0	39.0	33.0	39.0
14	37.87676228789332	40.0	37.0	41.0	33.0	41.0
15	37.92462225704972	40.0	37.0	41.0	33.0	41.0
16	37.94210333288533	40.0	37.0	41.0	33.0	41.0
17	37.85759096469388	40.0	36.0	41.0	33.0	41.0
18	37.822806494012376	39.0	36.0	41.0	33.0	41.0
19	37.727872452384915	39.0	36.0	41.0	33.0	41.0
20	37.70789473084747	39.0	36.0	41.0	33.0	41.0
21	37.75565674766338	39.0	36.0	41.0	33.0	41.0
22	37.83027137354239	40.0	36.0	41.0	33.0	41.0
23	37.80564888869653	40.0	36.0	41.0	33.0	41.0
24	37.79380348938128	40.0	36.0	41.0	33.0	41.0
25	37.602274772260806	39.0	36.0	41.0	33.0	41.0
26	37.60228844002925	39.0	36.0	41.0	33.0	41.0
27	37.58774137848557	40.0	36.0	41.0	33.0	41.0
28	37.48741084628544	40.0	36.0	41.0	33.0	41.0
29	37.42933877614245	40.0	36.0	41.0	33.0	41.0
30	37.33468720172943	39.0	36.0	41.0	32.0	41.0
31	37.35312274339448	39.0	36.0	41.0	32.0	41.0
32	37.25261680816604	39.0	36.0	41.0	32.0	41.0
33	37.147131249302376	39.0	35.0	41.0	32.0	41.0
34	37.091703892352655	39.0	35.0	41.0	32.0	41.0
35	37.02334454849666	39.0	35.0	41.0	32.0	41.0
36	36.960122007612945	39.0	35.0	41.0	31.0	41.0
37	36.88404493051079	39.0	35.0	41.0	31.0	41.0
38	36.807958741563	39.0	35.0	41.0	31.0	41.0
39	36.725874680231165	39.0	35.0	41.0	31.0	41.0
40	36.60913143609521	39.0	35.0	41.0	31.0	41.0
41	36.509994555672236	39.0	35.0	41.0	30.0	41.0
42	36.556610757900536	39.0	35.0	41.0	31.0	41.0
43	36.47350161393565	39.0	35.0	41.0	31.0	41.0
44	36.33513823808797	39.0	35.0	41.0	30.0	41.0
45	36.3595147031019	39.0	35.0	41.0	30.0	41.0
46	36.21784600525298	39.0	35.0	41.0	30.0	41.0
47	36.11700520969774	39.0	35.0	40.0	30.0	41.0
48	36.03184362250535	38.0	35.0	40.0	30.0	41.0
49	35.998198132527236	38.0	35.0	40.0	29.0	41.0
50	35.86563672438262	38.0	35.0	40.0	29.0	41.0
51	34.653503846337834	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	4.0
11	5.0
12	7.0
13	10.0
14	12.0
15	19.0
16	32.0
17	69.0
18	125.0
19	298.0
20	448.0
21	957.0
22	1392.0
23	1813.0
24	2303.0
25	2851.0
26	3733.0
27	4398.0
28	4807.0
29	5571.0
30	6846.0
31	8801.0
32	12030.0
33	16419.0
34	27993.0
35	34080.0
36	34744.0
37	51943.0
38	91431.0
39	125792.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.23530202351312	20.37590919134648	23.78055030991665	10.608238475223752
2	29.852000847401644	30.34996321092328	23.595807639826965	16.20222830184811
3	29.94220811911005	29.12054743968528	25.382412771162834	15.554831670041846
4	27.80889726166259	24.037732152741867	30.629241279394243	17.524129306201296
5	28.768374606197423	28.59365496629756	23.14408789286292	19.493882534642097
6	25.376945663786564	37.13942718382465	23.289421830615346	14.19420532177344
7	76.18596365740372	6.024068940224014	13.30056106189449	4.48940634047778
8	76.15794473210035	10.318253988140933	8.701129185469341	4.822672094289379
9	69.77099653977662	8.90386775067257	10.012779363492024	11.312356346058786
10	39.19232600361285	29.462013854561274	17.016371708630512	14.329288433195364
11	31.185291658788717	24.85916503602596	25.183546740351126	18.771996564834197
12	28.337384308035052	21.676397358475953	28.683862238005965	21.30235609548303
13	25.97103799867423	23.748203257940403	31.087339318297268	19.193419425088102
14	20.650631337003887	30.388688554838506	26.804771873554916	22.155908234602688
15	18.223235661941413	27.066509639193693	34.07784705311523	20.632407645749666
16	20.860887174849484	26.117283120989367	29.63468332919504	23.387146374966115
17	20.38638781381766	26.712286640439736	29.135354188829332	23.76597135691327
18	21.721273198189476	26.348723999917993	28.734205185095753	23.195797616796774
19	23.411976154299992	27.93782987728622	25.925934362820023	22.724259605593762
20	25.947802792325092	25.98425017483354	28.87953912284818	19.188407909993188
21	24.397877851153446	29.306656886619027	26.48972981099754	19.805735451229985
22	22.050666417609552	25.389474451523842	27.989767397360755	24.57009173350585
23	23.068231778017218	28.47451758472308	27.505017209998428	20.952233427261273
24	22.9094578679648	25.804519019838768	27.45376307834593	23.832260033850506
25	21.38345152156432	30.939727419138062	25.44437332142719	22.232447737870427
26	20.89209524612234	28.241482132809704	27.12231969365975	23.744102927408203
27	23.505828164259242	28.903457717619347	25.486743403593255	22.103970714528153
28	19.765643330470695	29.367706252320673	29.009838515315874	21.85681190189276
29	21.84428311415548	28.20526254644194	27.848989382421884	22.1014649569807
30	21.11419648328318	31.152716810671794	26.816845069010842	20.916241637034187
31	24.188988790151917	27.520051755283166	24.259150001480677	24.031809453084247
32	24.229536503192563	29.960887402645625	25.48036511165428	20.329210982507533
33	23.360038634225457	28.85789848948379	24.136595677796027	23.645467198494725
34	22.844991560152987	28.32417213187574	25.21475481162398	23.616081496347288
35	21.218299319572928	27.59408550100344	25.10882960620881	26.07878557321482
36	23.598768989655778	31.00829405748208	23.813580750314927	21.579356202547217
37	21.675941766194597	29.427388841178253	27.63713897159154	21.259530421035606
38	22.153402477055234	28.957673199100658	24.908141206271683	23.98078311757242
39	23.19329185924932	26.009991138730125	27.100223468014008	23.696493534006546
40	21.641088956670895	27.100223468014008	28.213235411365662	23.045452163949438
41	21.724234548018288	26.66900537371096	25.80816375808961	25.798596320181144
42	21.32604689411352	26.464672235522986	27.65718503197119	24.552095838392308
43	24.45186553649408	26.885411707354855	25.127053297463036	23.53566945868803
44	22.689634592210737	26.722309670629564	26.907052340719247	23.68100339644046
45	21.7579483768386	25.38264056730351	26.11158821747242	26.74782283838547
46	23.870301989343695	28.21733574189786	24.991970186041108	22.920392082717335
47	20.925353482661297	26.274462458057034	30.329005965980926	22.471178093300743
48	21.852939367501236	26.04552733667586	26.976757959766644	25.124775336056253
49	20.697101749702156	25.525924339789835	29.924667816277857	23.85230609423015
50	21.067042682162878	25.24368492149006	28.33989006558251	25.349382330764552
51	20.210301397073728	24.853697928649694	26.34690163079257	28.589099043484005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	445.0
2	702.0
3	1489.0
4	2276.0
5	1611.5
6	947.0
7	948.0
8	949.0
9	916.5
10	884.0
11	924.0
12	964.0
13	1004.5
14	1045.0
15	1018.0
16	991.0
17	1013.5
18	1036.0
19	1032.0
20	1028.0
21	1169.0
22	1310.0
23	1390.5
24	1471.0
25	2187.5
26	3153.5
27	3403.0
28	4275.5
29	5148.0
30	6208.0
31	7268.0
32	8130.5
33	8993.0
34	10358.0
35	11723.0
36	12417.5
37	13112.0
38	14806.0
39	16500.0
40	18235.0
41	19970.0
42	22374.5
43	24779.0
44	27179.0
45	29579.0
46	42807.0
47	56035.0
48	48358.5
49	40682.0
50	39119.0
51	37556.0
52	33070.5
53	28585.0
54	25950.5
55	23316.0
56	21374.5
57	19433.0
58	19240.5
59	19048.0
60	18924.5
61	18801.0
62	16390.5
63	13980.0
64	11513.5
65	9047.0
66	7358.0
67	5669.0
68	4643.0
69	3617.0
70	3107.5
71	2598.0
72	2146.5
73	1695.0
74	1302.5
75	676.5
76	443.0
77	317.5
78	192.0
79	159.5
80	127.0
81	90.5
82	54.0
83	33.5
84	13.0
85	8.5
86	4.0
87	5.5
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	438989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.726393519436684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.17656507577256	24.275314585898368
2	8.376007594852084	5.482330413418395
3	3.1467589110627374	3.0894621130269964
4	1.8557155591609715	2.429235105969738
5	1.3385126813168815	2.190234636976631
6	1.061732762563432	2.0848010520077707
7	0.9686862067881056	2.219112420013857
8	0.8038017146918468	2.104442498128268
9	0.7456086392838414	2.196097356662524
>10	7.400970929995208	44.12063863412956
>50	0.07887395261051112	1.6906110925303075
>100	0.03699397777307159	2.109095016529162
>500	0.005583996644991938	1.4023323195572974
>1k	0.0020939987418719767	0.8945322305565038
>5k	0.0020939987418719767	3.711760524594645
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	5694	1.2970712250193057	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	5530	1.259712657948149	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	5025	1.1446756069058677	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	1722	0.3922649542471452	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1183	0.26948283442181925	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	1011	0.23030189822524028	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT	995	0.2266571599743957	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT	876	0.19954941923373934	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	798	0.18178132026087215	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTC	785	0.17881997043206094	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA	738	0.16811355182020504	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT	729	0.166063386554105	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	615	0.14009462651683754	No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	603	0.13736107282870413	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5559228135556927E-4	0.0	0.0	0.15171222969140458	0.0
2	4.5559228135556927E-4	0.0	0.0	0.8767873454687931	0.0
3	6.83388422033354E-4	0.0	0.0	1.1717833476465243	0.0
4	6.83388422033354E-4	0.0	0.0	1.7469686028579303	0.0
5	6.83388422033354E-4	0.0	0.0	3.8283419402308487	0.0
6	6.83388422033354E-4	0.0	0.0	4.593053584486172	0.0
7	6.83388422033354E-4	0.0	0.0	5.369382831916061	0.0
8	6.83388422033354E-4	0.0	0.0	6.351639790518669	0.0
9	6.83388422033354E-4	0.0	0.0	6.668732018342145	0.0
10	6.83388422033354E-4	0.0	0.0	8.675160425432072	0.0
11	6.83388422033354E-4	0.0	0.0	9.681563774946525	0.0
12	6.83388422033354E-4	0.0	0.0	11.814191243971944	0.0
13	6.83388422033354E-4	0.0	0.0	12.219896170519078	0.0
14	6.83388422033354E-4	0.0	0.0	12.406689005874863	0.0
15	6.83388422033354E-4	0.0	0.0	12.81216613628132	0.0
16	6.83388422033354E-4	0.0	0.0	13.35796568934529	0.0
17	6.83388422033354E-4	0.0	0.0	13.98121593023971	0.0
18	6.83388422033354E-4	0.0	0.0	14.647291845581552	0.0
19	6.83388422033354E-4	0.0	0.0	15.255507541191237	0.0
20	6.83388422033354E-4	0.0	0.0	15.712694395531551	0.0
21	6.83388422033354E-4	0.0	0.0	16.199950340441333	0.0
22	6.83388422033354E-4	0.0	0.0	16.76579595388495	0.0
23	6.83388422033354E-4	0.0	0.0	17.312278895370955	0.0
24	6.83388422033354E-4	0.0	0.0	17.732334978780788	0.0
25	6.83388422033354E-4	0.0	0.0	18.120271806355056	0.0
26	6.83388422033354E-4	0.0	0.0	18.489757146534423	0.0
27	6.83388422033354E-4	0.0	0.0	18.893867500096814	0.0
28	6.83388422033354E-4	0.0	0.0	19.266769782386348	0.0
29	6.83388422033354E-4	0.0	0.0	19.650378483287735	0.0
30	6.83388422033354E-4	0.0	0.0	20.123283271334817	0.0
31	6.83388422033354E-4	0.0	0.0	20.526710236475175	0.0
32	6.83388422033354E-4	0.0	0.0	20.91988637528503	0.0
33	6.83388422033354E-4	0.0	0.0	21.285271384932198	0.0
34	6.83388422033354E-4	0.0	0.0	21.662957386175965	0.0
35	6.83388422033354E-4	0.0	0.0	22.091897519072234	0.0
36	6.83388422033354E-4	0.0	0.0	22.51969867126511	0.0
37	6.83388422033354E-4	0.0	0.0	22.93223748203258	0.0
38	6.83388422033354E-4	0.0	0.0	23.317440755918714	0.0
39	9.111845627111385E-4	0.0	0.0	23.719273148074326	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	65	0.0	45.000004	31
CTCGTAT	20	7.0285174E-4	45.0	43
CGCGGGT	20	7.0285174E-4	45.0	4
GGGTACG	50	2.1827873E-11	45.0	7
TACGGTT	25	3.8868522E-5	45.0	33
TACGGAT	20	7.0285174E-4	45.0	18
TAACGCC	25	3.8868522E-5	45.0	12
TCTCGTA	25	3.8868522E-5	45.0	28
CGGGCTA	25	3.8868522E-5	45.0	6
GACGGGC	40	6.7993824E-9	45.0	4
TCGTACG	25	3.8868522E-5	45.0	30
ATAACGC	25	3.8868522E-5	45.0	11
TCGAGAG	20	7.0285174E-4	45.0	1
AGGGCGC	25	3.8868522E-5	45.0	6
TCGAAAG	20	7.0285174E-4	45.0	1
TACGGCT	665	0.0	42.969925	7
ACGGCTG	705	0.0	40.531918	8
CATGCGG	45	1.9235813E-8	40.0	2
TATACTC	45	1.9235813E-8	40.0	43
CGGCTGT	725	0.0	39.724136	9
>>END_MODULE