FastQCFastQC Report
Sat 18 Jun 2016
SRR3550721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481485
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC10400.21599842154999635RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.20935231627153494No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC8130.16885261223091064RNA PCR Primer, Index 38 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT6760.14039897400749762RNA PCR Primer, Index 38 (96% over 26bp)
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC6410.13312979635918046RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC5930.12316063844148831No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5520.1146453160534596No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG5480.11381455289365193No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC4990.10363770418600787RNA PCR Primer, Index 27 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4930.10239155944629635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG551.8189894E-1245.0000041
GTGCACG253.887417E-545.01
CGCATCG253.887417E-545.021
AATGCGA207.029199E-445.017
CTAACGG253.887417E-545.02
TACGCGG253.887417E-545.02
CATCGTT253.887417E-545.036
TATAGCG502.1827873E-1145.01
CCAACGG253.887417E-545.02
GCTAACG207.029199E-445.01
TATCGAG253.887417E-545.01
GCGTAAG207.029199E-445.01
TCGTAAG302.162864E-644.9999961
CCTCGTG800.039.37515
CACATAG1200.039.37499634
ATATCAC1200.039.37499630
ACCTAGG356.240729E-638.5714262
ACTACGG356.240729E-638.5714262
GTACTAG356.240729E-638.5714261
CGAATAT700.038.57142614
Produced by FastQC (version 0.11.5)