##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550721_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481485
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.812413678515426	33.0	31.0	34.0	30.0	34.0
2	31.850045172746814	33.0	31.0	34.0	30.0	34.0
3	31.94448632875375	33.0	31.0	34.0	30.0	34.0
4	35.6928585521875	37.0	35.0	37.0	33.0	37.0
5	35.719756586394176	37.0	35.0	37.0	35.0	37.0
6	35.67189216694186	37.0	35.0	37.0	33.0	37.0
7	35.96215458425496	37.0	35.0	37.0	35.0	37.0
8	35.788369315762694	37.0	35.0	37.0	35.0	37.0
9	37.589623768134004	39.0	37.0	39.0	35.0	39.0
10	36.93600215998421	39.0	37.0	39.0	32.0	39.0
11	36.94824553205188	39.0	37.0	39.0	33.0	39.0
12	36.9434291826329	39.0	37.0	39.0	33.0	39.0
13	36.85856464895064	39.0	37.0	39.0	33.0	39.0
14	37.78907754135643	40.0	37.0	41.0	33.0	41.0
15	37.9628420407697	40.0	37.0	41.0	33.0	41.0
16	37.89447023271753	40.0	37.0	41.0	33.0	41.0
17	37.939854824137825	40.0	37.0	41.0	33.0	41.0
18	37.89482953778415	39.0	37.0	41.0	33.0	41.0
19	37.81307205831958	39.0	36.0	41.0	33.0	41.0
20	37.89088964349876	40.0	36.0	41.0	33.0	41.0
21	37.813848821874	39.0	36.0	41.0	33.0	41.0
22	37.909741736502696	40.0	36.0	41.0	33.0	41.0
23	37.93202488135664	40.0	36.0	41.0	34.0	41.0
24	37.929345670166256	40.0	36.0	41.0	34.0	41.0
25	37.63171646053356	39.0	36.0	41.0	33.0	41.0
26	37.7235386356792	39.0	36.0	41.0	33.0	41.0
27	37.73355556247858	40.0	36.0	41.0	33.0	41.0
28	37.62883578927693	40.0	36.0	41.0	33.0	41.0
29	37.63869071726014	40.0	36.0	41.0	33.0	41.0
30	37.499714425163816	39.0	36.0	41.0	33.0	41.0
31	37.50336978306697	40.0	36.0	41.0	33.0	41.0
32	37.53116088767044	40.0	36.0	41.0	33.0	41.0
33	37.45693012243372	40.0	36.0	41.0	33.0	41.0
34	37.436790346532085	40.0	36.0	41.0	33.0	41.0
35	37.39577141551658	40.0	36.0	41.0	33.0	41.0
36	37.29193848198802	40.0	35.0	41.0	32.0	41.0
37	37.2155269634568	40.0	35.0	41.0	32.0	41.0
38	37.15872560931285	39.0	35.0	41.0	32.0	41.0
39	37.094050697321826	39.0	35.0	41.0	32.0	41.0
40	36.98733501562873	39.0	35.0	41.0	32.0	41.0
41	36.86736450772091	39.0	35.0	41.0	31.0	41.0
42	36.88973903652243	39.0	35.0	41.0	31.0	41.0
43	36.816486494906385	39.0	35.0	41.0	31.0	41.0
44	36.67722566642782	39.0	35.0	41.0	31.0	41.0
45	36.6513827014341	39.0	35.0	41.0	31.0	41.0
46	36.54841999231544	39.0	35.0	41.0	31.0	41.0
47	36.507334600247155	39.0	35.0	41.0	31.0	41.0
48	36.408438476795745	39.0	35.0	40.0	31.0	41.0
49	36.32975897483826	39.0	35.0	40.0	31.0	41.0
50	36.19467688505353	38.0	35.0	40.0	31.0	41.0
51	34.93491801406066	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	3.0
11	4.0
12	11.0
13	19.0
14	15.0
15	13.0
16	31.0
17	56.0
18	115.0
19	241.0
20	357.0
21	594.0
22	882.0
23	1302.0
24	1890.0
25	2539.0
26	3419.0
27	4198.0
28	4831.0
29	5715.0
30	7197.0
31	9316.0
32	12408.0
33	17651.0
34	30997.0
35	36553.0
36	38290.0
37	57961.0
38	103493.0
39	141312.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.80393989428539	22.55418133482871	27.17426295730916	10.467615813576748
2	30.94302003177669	25.766327092225094	26.309646198739316	16.981006677258897
3	31.52496962522197	24.58082806317954	27.229301016646417	16.664901294952074
4	29.18138675140451	25.964048724259325	26.126255231211772	18.728309293124397
5	25.398299012430293	30.602614827045493	25.3775299334351	18.62155622708911
6	25.953248803181822	35.52987112786484	23.96772485124147	14.54915521771187
7	80.29699782963124	6.787335015628732	8.443045993125436	4.472621161614588
8	81.42060500327113	6.203100823493982	7.628482714934007	4.747811458300881
9	74.68623113908014	8.846381507212064	10.108310746959926	6.359076606747874
10	39.726678920423275	29.844958825300893	16.656593663354	13.771768590921834
11	30.71435247203963	25.723335098705046	24.65538905677228	18.906923372483046
12	28.365577328473368	22.380551834428903	29.520961192975896	19.732909644121833
13	25.6263434997975	23.880287028671713	31.053511532031113	19.43985793949967
14	21.12319179205998	28.00751840659626	29.706013686823056	21.163276114520702
15	20.215790730760045	25.94203350052442	34.15433502601327	19.687840742702264
16	23.0241855924899	25.50609053241534	30.605937879684724	20.863785995410034
17	23.902509943196566	25.701112184180193	27.1032327071456	23.293145165477636
18	24.506890141956656	25.964256415049274	28.84160461904317	20.687248823950902
19	25.586674558916684	26.522113876860132	26.80353489724498	21.087676666978204
20	25.16090843951525	27.2764468259655	27.477699201428912	20.084945533090334
21	25.09818582094977	26.876226673728155	28.3261160783825	19.69947142693957
22	23.156900007269176	25.420937308535052	28.152071196402794	23.270091487792975
23	22.739233828675868	26.5187908242209	28.057987268554573	22.683988078548655
24	22.643696065297984	26.170077987891627	28.23556289396347	22.95066305284692
25	22.39301328182602	27.553090958181457	27.17426295730916	22.879632802683364
26	21.689564576258867	27.53730645814511	26.913818706709453	23.85931025888657
27	22.48107417676563	27.829942781187366	27.709482123015256	21.979500919031743
28	20.95122381797979	27.401476681516556	28.650529092287403	22.996770408216246
29	22.879840493473317	26.077863277152975	27.724435859891795	23.317860369481917
30	22.903101861947935	27.182570588907236	27.899103814241354	22.015223734903476
31	24.29317631909613	27.868365577328476	25.80059607256716	22.037862031008235
32	25.414914275626447	26.900318805362577	25.736211927682067	21.94855499132891
33	24.05620112776099	27.08599437157959	26.22573911959874	22.632065381060677
34	23.273622231222156	27.1080095953145	27.477076129059057	22.141292044404288
35	23.15087697436057	26.79917339065599	26.84008847627652	23.209861158706918
36	24.99745578782309	27.580713833245063	26.593559508603594	20.828270870328254
37	23.510597422557296	28.94129619822009	26.751196818177096	20.796909561045513
38	22.503089400500535	27.472299240890163	27.098248128186757	22.92636323042255
39	23.30976042867379	25.9075568293924	27.734405017809483	23.048277724124326
40	23.408413553900953	25.844626520036968	29.442661765164026	21.304298160898057
41	21.709087510514347	26.186070178717923	27.93794199196237	24.16690031880536
42	23.6678193505509	25.68906611836298	28.005026117116838	22.63808841396928
43	23.56708931742422	25.15675462371621	28.26692420324621	23.00923185561336
44	23.02833940828894	25.13473939998131	27.581959977984777	24.254961213744977
45	22.29145248553953	25.485944525790003	27.098455818976706	25.12414716969376
46	22.945055401518218	26.909664890910413	27.285585220723384	22.85969448684798
47	21.790917681755403	26.276000290767104	29.364985409722006	22.568096617755483
48	21.8937246227816	25.596436026044422	28.7745204938887	23.73531885728527
49	22.96790138841293	24.966509860120254	28.83392005981495	23.23166869165187
50	21.53338110221502	24.393283279852955	29.71972127895988	24.353614338972136
51	21.383012970289833	24.869725952002657	27.245293207472717	26.501967870234793
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	200.0
1	343.0
2	486.0
3	1223.0
4	1960.0
5	1385.0
6	810.0
7	822.5
8	835.0
9	874.0
10	913.0
11	900.5
12	888.0
13	987.0
14	1086.0
15	1060.0
16	1034.0
17	1114.5
18	1195.0
19	1258.5
20	1322.0
21	1447.5
22	1573.0
23	1710.5
24	1848.0
25	2320.0
26	3365.0
27	3938.0
28	5154.0
29	6370.0
30	7270.0
31	8170.0
32	9073.0
33	9976.0
34	10959.5
35	11943.0
36	13428.5
37	14914.0
38	16633.0
39	18352.0
40	19838.0
41	21324.0
42	24574.5
43	27825.0
44	30433.5
45	33042.0
46	38345.5
47	43649.0
48	46343.5
49	49038.0
50	47465.0
51	45892.0
52	40448.0
53	35004.0
54	31552.5
55	28101.0
56	26030.5
57	23960.0
58	22826.0
59	21692.0
60	20395.0
61	19098.0
62	16540.0
63	13982.0
64	11833.0
65	9684.0
66	8157.0
67	6630.0
68	5631.5
69	4633.0
70	3936.0
71	3239.0
72	2495.5
73	1752.0
74	1473.0
75	882.5
76	571.0
77	406.5
78	242.0
79	227.5
80	213.0
81	137.0
82	61.0
83	48.0
84	35.0
85	23.0
86	11.0
87	6.0
88	1.0
89	2.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	481485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.66115687825149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32474547383703	25.415205059265254
2	9.315818369415844	6.45794083903239
3	3.4672064678349663	3.605321619327479
4	1.9142454271635874	2.653998442175705
5	1.3246462194772446	2.2956885210741764
6	1.0624035485634917	2.209448163886216
7	0.8738593687751494	2.120228366945169
8	0.7822226723055178	2.169019420680532
9	0.7005628863944271	2.185408809755812
>10	7.131586736148073	45.97313651465079
>50	0.0660661480077032	1.5881699327102015
>100	0.031831871312802455	2.104236686306818
>500	0.0036036080731474476	0.7958544570390744
>1k	0.0012012026910491493	0.4263431671504119
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1040	0.21599842154999635	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1008	0.20935231627153494	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	813	0.16885261223091064	RNA PCR Primer, Index 38 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	676	0.14039897400749762	RNA PCR Primer, Index 38 (96% over 26bp)
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	641	0.13312979635918046	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	593	0.12316063844148831	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	552	0.1146453160534596	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	548	0.11381455289365193	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	499	0.10363770418600787	RNA PCR Primer, Index 27 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	493	0.10239155944629635	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.153815799038392E-4	0.0	0.0	0.1547296385141801	0.0
2	4.153815799038392E-4	0.0	0.0	0.7451945543474875	0.0
3	4.153815799038392E-4	0.0	0.0	1.0658691340332513	0.0
4	4.153815799038392E-4	0.0	0.0	1.5007736481925709	0.0
5	4.153815799038392E-4	0.0	0.0	2.6484729534668787	0.0
6	4.153815799038392E-4	0.0	0.0	3.376221481458405	0.0
7	4.153815799038392E-4	0.0	0.0	4.100854647600652	0.0
8	4.153815799038392E-4	0.0	0.0	5.347414768892073	0.0
9	4.153815799038392E-4	0.0	0.0	5.840057322658026	0.0
10	4.153815799038392E-4	0.0	0.0	6.944349252832383	0.0
11	4.153815799038392E-4	0.0	0.0	8.214378433388372	0.0
12	4.153815799038392E-4	0.0	0.0	9.35688546891388	0.0
13	4.153815799038392E-4	0.0	0.0	9.840805009501853	0.0
14	4.153815799038392E-4	0.0	0.0	10.040188167855696	0.0
15	4.153815799038392E-4	0.0	0.0	10.359201221221845	0.0
16	6.230723698557588E-4	0.0	0.0	10.966073709461353	0.0
17	6.230723698557588E-4	0.0	0.0	11.708983665119371	0.0
18	6.230723698557588E-4	0.0	0.0	12.578169621068154	0.0
19	6.230723698557588E-4	0.0	0.0	13.098850431477617	0.0
20	6.230723698557588E-4	0.0	0.0	13.627838873485155	0.0
21	6.230723698557588E-4	0.0	0.0	14.238865177523703	0.0
22	6.230723698557588E-4	0.0	0.0	14.887068132963643	0.0
23	6.230723698557588E-4	0.0	0.0	15.495602147522769	0.0
24	6.230723698557588E-4	0.0	0.0	15.987413938128913	0.0
25	6.230723698557588E-4	0.0	0.0	16.431041465466215	0.0
26	6.230723698557588E-4	0.0	0.0	16.866569051995388	0.0
27	6.230723698557588E-4	0.0	0.0	17.33408102017716	0.0
28	6.230723698557588E-4	0.0	0.0	17.774593185665182	0.0
29	6.230723698557588E-4	0.0	0.0	18.260589634152673	0.0
30	6.230723698557588E-4	0.0	0.0	18.858323727634296	0.0
31	6.230723698557588E-4	0.0	0.0	19.363427728797365	0.0
32	6.230723698557588E-4	0.0	0.0	19.83654734830784	0.0
33	6.230723698557588E-4	0.0	0.0	20.304474698069516	0.0
34	6.230723698557588E-4	0.0	0.0	20.782163514958928	0.0
35	6.230723698557588E-4	0.0	0.0	21.272313779245458	0.0
36	6.230723698557588E-4	0.0	0.0	21.736087313208095	0.0
37	6.230723698557588E-4	0.0	0.0	22.188022472143473	0.0
38	6.230723698557588E-4	0.0	0.0	22.689388039087408	0.0
39	6.230723698557588E-4	0.0	0.0	23.318898823431674	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGCG	55	1.8189894E-12	45.000004	1
GTGCACG	25	3.887417E-5	45.0	1
CGCATCG	25	3.887417E-5	45.0	21
AATGCGA	20	7.029199E-4	45.0	17
CTAACGG	25	3.887417E-5	45.0	2
TACGCGG	25	3.887417E-5	45.0	2
CATCGTT	25	3.887417E-5	45.0	36
TATAGCG	50	2.1827873E-11	45.0	1
CCAACGG	25	3.887417E-5	45.0	2
GCTAACG	20	7.029199E-4	45.0	1
TATCGAG	25	3.887417E-5	45.0	1
GCGTAAG	20	7.029199E-4	45.0	1
TCGTAAG	30	2.162864E-6	44.999996	1
CCTCGTG	80	0.0	39.375	15
CACATAG	120	0.0	39.374996	34
ATATCAC	120	0.0	39.374996	30
ACCTAGG	35	6.240729E-6	38.571426	2
ACTACGG	35	6.240729E-6	38.571426	2
GTACTAG	35	6.240729E-6	38.571426	1
CGAATAT	70	0.0	38.571426	14
>>END_MODULE