##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550720_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	157471
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46710187907615	33.0	31.0	34.0	31.0	34.0
2	32.541109156606616	34.0	31.0	34.0	31.0	34.0
3	32.60299991744512	34.0	31.0	34.0	31.0	34.0
4	36.17617212058093	37.0	35.0	37.0	35.0	37.0
5	36.15630179525119	37.0	35.0	37.0	35.0	37.0
6	36.13269744905411	37.0	35.0	37.0	35.0	37.0
7	36.298131084453644	37.0	37.0	37.0	35.0	37.0
8	36.14736046637159	37.0	37.0	37.0	35.0	37.0
9	38.052003225990816	39.0	39.0	39.0	35.0	39.0
10	37.40035943126036	39.0	37.0	39.0	34.0	39.0
11	37.45096557461374	39.0	37.0	39.0	35.0	39.0
12	37.48497818645973	39.0	37.0	39.0	35.0	39.0
13	37.4230810752456	39.0	37.0	39.0	35.0	39.0
14	38.621034984219314	40.0	38.0	41.0	35.0	41.0
15	38.708562211454804	40.0	38.0	41.0	35.0	41.0
16	38.7093814099104	40.0	38.0	41.0	35.0	41.0
17	38.67670872732122	40.0	38.0	41.0	35.0	41.0
18	38.61517993789332	40.0	38.0	41.0	35.0	41.0
19	38.50240996754958	40.0	37.0	41.0	35.0	41.0
20	38.51653955331458	40.0	38.0	41.0	35.0	41.0
21	38.51735875177017	40.0	38.0	41.0	35.0	41.0
22	38.60407948130132	40.0	38.0	41.0	35.0	41.0
23	38.600148598789616	40.0	38.0	41.0	35.0	41.0
24	38.6074642315093	40.0	38.0	41.0	35.0	41.0
25	38.40733849407192	40.0	37.0	41.0	35.0	41.0
26	38.45314375345302	40.0	38.0	41.0	35.0	41.0
27	38.47520495837329	40.0	38.0	41.0	35.0	41.0
28	38.34694642188085	40.0	37.0	41.0	35.0	41.0
29	38.322649884740684	40.0	37.0	41.0	35.0	41.0
30	38.230455131421024	40.0	37.0	41.0	34.0	41.0
31	38.236208571737016	40.0	37.0	41.0	34.0	41.0
32	38.19028265521906	40.0	37.0	41.0	34.0	41.0
33	38.054879946148816	40.0	37.0	41.0	34.0	41.0
34	37.989426624584844	40.0	37.0	41.0	34.0	41.0
35	37.93784252338526	40.0	37.0	41.0	34.0	41.0
36	37.80449733601743	40.0	37.0	41.0	34.0	41.0
37	37.75747915489201	40.0	37.0	41.0	34.0	41.0
38	37.66745623003601	40.0	37.0	41.0	34.0	41.0
39	37.598573705634685	40.0	37.0	41.0	33.0	41.0
40	37.51149100469293	40.0	36.0	41.0	33.0	41.0
41	37.333483625556454	40.0	36.0	41.0	33.0	41.0
42	37.35022956607883	40.0	36.0	41.0	33.0	41.0
43	37.2652869417226	40.0	36.0	41.0	33.0	41.0
44	37.22044058906084	40.0	36.0	41.0	33.0	41.0
45	37.19482952416635	40.0	35.0	41.0	33.0	41.0
46	37.076642683414725	40.0	35.0	41.0	33.0	41.0
47	37.04678956760292	40.0	35.0	41.0	32.0	41.0
48	36.939265007525194	39.0	35.0	41.0	32.0	41.0
49	36.822722914060364	39.0	35.0	41.0	32.0	41.0
50	36.70016701487893	39.0	35.0	41.0	32.0	41.0
51	35.54419543916023	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	1.0
12	1.0
13	4.0
14	4.0
15	5.0
16	7.0
17	9.0
18	31.0
19	60.0
20	113.0
21	207.0
22	281.0
23	406.0
24	578.0
25	809.0
26	1019.0
27	1195.0
28	1287.0
29	1312.0
30	1410.0
31	1740.0
32	2359.0
33	3416.0
34	7630.0
35	10626.0
36	8997.0
37	15453.0
38	35452.0
39	63028.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.60557181957313	22.55526414387411	26.796044986060924	10.043119050491836
2	30.9409351563145	27.781623283017193	25.380546259311238	15.896895301357075
3	29.802947844364997	26.818271300747437	27.501571717967117	15.877209136920447
4	26.7268258917515	25.647897073111874	30.100145423601806	17.52513161153482
5	26.224511179836284	30.016955502917998	25.97430638022239	17.784226937023327
6	24.416559239479017	36.56673292225235	25.46056099218269	13.556146846085944
7	79.3168265902928	5.996659702421399	10.818499914269928	3.8680137930158573
8	80.94315778778315	7.112420699684387	7.910662915711464	4.033758596821002
9	74.07395647452547	7.98940757345797	10.162506112236539	7.774129839780023
10	39.00718227483156	28.351887014116883	18.22811819319113	14.412812517860432
11	30.749153812448004	25.952080065535878	25.269414685878672	18.029351436137446
12	26.72873100443891	22.72291406036667	29.93694077004655	20.611414165147867
13	24.842034406335134	24.808377415524127	32.24212712181925	18.10746105632148
14	20.860348889636825	28.59066113760629	30.47735773571007	20.07163223704682
15	20.407567107594417	26.133700808402814	35.09916111537997	18.359570968622794
16	22.980104273167758	25.775539623168708	30.34018962221615	20.90416648144738
17	22.835950746486656	26.143226371839894	28.70179271103886	22.319030170634594
18	23.251265312343225	26.275949222396505	29.717852810993772	20.7549326542665
19	24.002514748747387	28.69353722272672	27.322491125350062	19.981456903175822
20	24.018390687809184	27.880054105200326	28.835150599157938	19.266404607832555
21	23.313498993465466	29.896298366048352	28.55255888385798	18.237643756628206
22	21.586831861104585	26.57505191432073	28.79069796978491	23.04741825478977
23	20.73080122689257	28.600186701043366	28.720843837913012	21.94816823415105
24	21.816715458719383	26.622679731506118	29.18124607070508	22.379358739069417
25	20.76826844307841	30.664693816639254	26.88050498186968	21.68653275841266
26	19.61440519206711	29.05931885871049	28.508106254484954	22.818169694737445
27	21.35567818836484	28.59320128785618	28.600821738605838	21.450298785173143
28	19.57312775050644	27.789878771329324	31.217811533552208	21.419181944612024
29	20.99942211581815	26.491861993636924	30.222072635596398	22.28664325494853
30	21.974839811774867	28.06040477294232	29.858831149862514	20.1059242654203
31	22.324110471134368	28.47889452661125	26.94400873811686	22.252986264137522
32	23.540207403267903	27.835601475827293	27.114198804859306	21.509992316045494
33	22.74958563799049	27.236126016853895	26.948454001054163	23.065834344101454
34	21.32329127267878	26.675387849191278	28.8834134539058	23.117907424224143
35	21.151831130811388	26.59219792850747	29.148224117456543	23.10774682322459
36	22.515256777438385	28.08136101250389	28.94691721015298	20.456464999904746
37	20.466625600904294	28.811019171784015	29.509560490503013	21.212794736808682
38	21.94054778340139	28.638923992354147	26.753497469375315	22.66703075486915
39	22.4428624953166	26.269598846771785	27.25454210616558	24.032996551746034
40	24.601990207720785	24.9487207168304	27.994360866445252	22.454928209003562
41	20.648246343771234	25.508823846930547	28.938026684278373	24.904903125019846
42	21.6338246407275	26.074642315092937	30.12935715147551	22.162175892704052
43	20.98291113919388	25.719021280108716	30.230963161471003	23.067104419226396
44	21.438868109048652	26.36421944358009	28.597011513231006	23.599900934140255
45	20.627925141772135	26.383270570454243	27.58222148840104	25.406582799372586
46	22.40412520400582	28.617332715230106	26.648716271567462	22.329825809196613
47	20.711750100018417	25.630116021362664	31.03682582824774	22.62130805037118
48	21.306780296054512	25.56597722755301	29.55401311987604	23.573229356516435
49	21.315035784366646	24.698515917216504	30.544036679769608	23.442411618647245
50	20.797480170952113	24.776625537400538	29.990283925294182	24.43561036635317
51	20.586647700211465	25.30053152643979	27.58920690158823	26.523613871760514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	119.0
2	171.0
3	560.0
4	949.0
5	659.0
6	369.0
7	372.0
8	375.0
9	393.0
10	411.0
11	431.5
12	452.0
13	462.5
14	473.0
15	478.5
16	484.0
17	511.5
18	539.0
19	490.5
20	442.0
21	511.0
22	580.0
23	717.5
24	855.0
25	954.0
26	1272.0
27	1491.0
28	1841.0
29	2191.0
30	2464.0
31	2737.0
32	3272.0
33	3807.0
34	4302.0
35	4797.0
36	5272.0
37	5747.0
38	6148.5
39	6550.0
40	7220.5
41	7891.0
42	8704.0
43	9517.0
44	10264.0
45	11011.0
46	14419.5
47	17828.0
48	17065.5
49	16303.0
50	15371.0
51	14439.0
52	12444.5
53	10450.0
54	9324.5
55	8199.0
56	7451.0
57	6703.0
58	6342.5
59	5982.0
60	5431.5
61	4881.0
62	4162.0
63	3443.0
64	2896.5
65	2350.0
66	1916.0
67	1482.0
68	1193.5
69	905.0
70	760.5
71	616.0
72	518.5
73	421.0
74	321.5
75	181.5
76	141.0
77	113.0
78	85.0
79	61.0
80	37.0
81	25.0
82	13.0
83	10.0
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	157471.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.1724126982111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.08592909489032	36.66897396981032
2	9.882668624299113	9.916746575559944
3	4.990697026845722	7.511859326479161
4	3.1781994000531597	6.378317277466962
5	2.236510688926298	5.610556864438532
6	1.7188350399331704	5.174286059020391
7	1.27330489703444	4.471934514926558
8	0.9189059197286317	3.6882981628363316
9	0.6885465844798562	3.109143905862032
>10	1.9846342729125266	13.213226562351164
>50	0.024048502031465566	0.7823662769652825
>100	0.012657106332350298	1.2865861015679076
>500	0.0025314212664700594	0.8420598078376337
>1k	0.0025314212664700594	1.3456445948777869
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	1094	0.6947310933441713	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC	1025	0.6509135015336157	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTC	787	0.49977456166532247	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	539	0.3422852461723111	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	414	0.26290555086333356	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTT	408	0.2590953254885026	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	194	0.12319728711953312	No Hit
CTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGCT	170	0.10795638562020944	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCT	168	0.10668631049526581	No Hit
GAACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCT	163	0.10351112268290669	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11557683636987127	0.0
2	0.0	0.0	0.0	0.6559938020333903	0.0
3	0.0	0.0	0.0	0.9690673203319976	0.0
4	0.0	0.0	0.0	1.4580462434352992	0.0
5	0.0	0.0	0.0	2.853223768185888	0.0
6	0.0	0.0	0.0	3.8813495818277652	0.0
7	0.0	0.0	0.0	4.64720488216878	0.0
8	0.0	0.0	0.0	5.790907532180529	0.0
9	0.0	0.0	0.0	6.34974058715573	0.0
10	0.0	0.0	0.0	8.169758241199967	0.0
11	0.0	0.0	0.0	9.54207441370157	0.0
12	0.0	0.0	0.0	11.275091921687167	0.0
13	0.0	0.0	0.0	11.854881216223939	0.0
14	0.0	0.0	0.0	12.129852480774238	0.0
15	0.0	0.0	0.0	12.49690419188295	0.0
16	0.0	0.0	0.0	13.105905214293426	0.0
17	0.0	0.0	0.0	13.781585180763441	0.0
18	0.0	0.0	0.0	14.608404087101752	0.0
19	0.0	0.0	0.0	15.178032780638976	0.0
20	0.0	0.0	0.0	15.691143131116206	0.0
21	0.0	0.0	0.0	16.267122200278145	0.0
22	0.0	0.0	0.0	16.96185329362232	0.0
23	0.0	0.0	0.0	17.64261356059211	0.0
24	0.0	0.0	0.0	18.150643610569563	0.0
25	0.0	0.0	0.0	18.64660794686006	0.0
26	0.0	0.0	0.0	19.102564916714822	0.0
27	0.0	0.0	0.0	19.559791961694533	0.0
28	0.0	0.0	0.0	20.024004419861434	0.0
29	0.0	0.0	0.0	20.5498155215881	0.0
30	0.0	0.0	0.0	21.129604816124875	0.0
31	0.0	0.0	0.0	21.66938674422592	0.0
32	0.0	0.0	0.0	22.14375980339237	0.0
33	0.0	0.0	0.0	22.644804440182636	0.0
34	0.0	0.0	0.0	23.1414038140356	0.0
35	0.0	0.0	0.0	23.68563100507395	0.0
36	0.0	0.0	0.0	24.182865416489385	0.0
37	0.0	0.0	0.0	24.680734865467294	0.0
38	0.0	0.0	0.0	25.18876491544475	0.0
39	0.0	0.0	0.0	25.764108947044218	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	25	3.8754348E-5	45.0	1
ATAGCGG	25	3.8754348E-5	45.0	2
ACGGGCA	20	7.014732E-4	45.0	5
ATCCGGG	25	3.8754348E-5	45.0	3
AACGGGC	25	3.8754348E-5	45.0	4
AGTGCGG	25	3.8754348E-5	45.0	2
CGAATAT	20	7.014732E-4	45.0	14
GCGGCTA	20	7.014732E-4	45.0	24
ACGTAAG	20	7.014732E-4	45.0	1
CGGTAGT	20	7.014732E-4	45.0	12
GGCGACT	20	7.014732E-4	45.0	10
CGCCGGT	20	7.014732E-4	45.0	28
GATTCAT	20	7.014732E-4	45.0	45
GTTATAG	20	7.014732E-4	45.0	1
AGCACGG	20	7.014732E-4	45.0	2
ACGGGAC	30	2.1535434E-6	44.999996	5
CGTTTTT	370	0.0	42.567566	1
AAGGGCC	75	0.0	42.0	5
CACGGGA	70	0.0	41.785717	4
TAACGGG	55	6.002665E-11	40.90909	3
>>END_MODULE