FastQCFastQC Report
Sat 18 Jun 2016
SRR3550719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538459
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107511.9966236983688637No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC79381.4742069498327635No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG54421.010661907406135No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC53350.9907903851546728No Hit
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT22610.41990197953790354No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC20290.37681606213286434TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG17140.3183157863458499No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT15740.2923156637738435No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT14370.26687268668552294No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC11660.2165438779925677No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT11390.211529568639395No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC10660.19797236186970593TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC10530.19555806477373391No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT10140.18831517348581786No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT9210.17104366349155647No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCAGTAT8650.1606436144627539No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT8320.15451501414220953TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTA7020.1303720431824893No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT207.0299447E-445.028
ATATCGA207.0299447E-445.025
CGAATGC253.8880346E-545.045
TCTCACG207.0299447E-445.023
CGGTCTA207.0299447E-445.031
GCGTAAG351.2103192E-745.01
CGTTTTT60600.044.368811
CGTAAGG700.041.7857132
CGTTCTG1050.040.7142871
CGAATAT501.0786607E-940.514
CACAACG451.9250365E-840.012
CGTTTCT2250.040.01
TACGGCT7650.039.7058837
TAGGGCG1650.039.5454525
AAGGGCG1150.039.1304365
CCGATGA2950.038.89830418
GCGATGT1400.038.5714269
TTACGAG356.242104E-638.5714261
GTTTTTT70550.038.0793762
CGTATGG659.094947E-1238.076922
Produced by FastQC (version 0.11.5)