##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	538459
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.786089562993656	33.0	31.0	34.0	30.0	34.0
2	31.856055892834924	33.0	31.0	34.0	30.0	34.0
3	31.919249190746186	33.0	31.0	34.0	30.0	34.0
4	35.6591086786552	37.0	35.0	37.0	33.0	37.0
5	35.676096044452784	37.0	35.0	37.0	33.0	37.0
6	35.60695057562414	37.0	35.0	37.0	33.0	37.0
7	35.899242096427024	37.0	35.0	37.0	35.0	37.0
8	35.689558536490246	37.0	35.0	37.0	35.0	37.0
9	37.53238036693602	39.0	37.0	39.0	35.0	39.0
10	36.973511446553964	39.0	37.0	39.0	32.0	39.0
11	36.91747561095645	39.0	37.0	39.0	33.0	39.0
12	37.02943213875151	39.0	37.0	39.0	33.0	39.0
13	37.018194514345566	39.0	37.0	39.0	33.0	39.0
14	38.161700333730145	40.0	37.0	41.0	33.0	41.0
15	38.19049918378187	40.0	37.0	41.0	33.0	41.0
16	38.16004932594682	40.0	37.0	41.0	33.0	41.0
17	38.08075452355704	40.0	37.0	41.0	33.0	41.0
18	37.85886019176947	39.0	37.0	41.0	33.0	41.0
19	37.59579280873753	39.0	37.0	41.0	33.0	41.0
20	37.421081270811705	39.0	35.0	41.0	33.0	41.0
21	37.44787811142538	39.0	35.0	41.0	33.0	41.0
22	37.551917601897266	39.0	35.0	41.0	33.0	41.0
23	37.52443175803543	39.0	35.0	41.0	33.0	41.0
24	37.48066426598868	39.0	35.0	41.0	33.0	41.0
25	37.280026891555345	39.0	35.0	41.0	33.0	41.0
26	37.31189189891895	39.0	35.0	41.0	33.0	41.0
27	37.321835088651135	39.0	35.0	41.0	33.0	41.0
28	37.17607097290602	39.0	35.0	41.0	33.0	41.0
29	37.12760117297696	39.0	35.0	41.0	33.0	41.0
30	36.89219049175517	39.0	35.0	41.0	32.0	41.0
31	36.8006384887243	39.0	35.0	41.0	31.0	41.0
32	36.48162441337224	39.0	35.0	41.0	31.0	41.0
33	36.032847440566506	39.0	35.0	41.0	29.0	41.0
34	35.70219273890863	39.0	35.0	41.0	25.0	41.0
35	35.3754603414559	39.0	35.0	41.0	23.0	41.0
36	35.220865469794354	39.0	35.0	41.0	22.0	41.0
37	35.09577888010043	39.0	35.0	41.0	21.0	41.0
38	35.00440330647273	39.0	35.0	41.0	21.0	41.0
39	34.91908761855591	39.0	35.0	41.0	20.0	41.0
40	34.75572141982955	39.0	35.0	41.0	19.0	41.0
41	34.641970883577024	38.0	34.0	40.0	18.0	41.0
42	34.62986596936814	39.0	35.0	40.0	18.0	41.0
43	34.58681533784374	38.0	34.0	40.0	18.0	41.0
44	34.48129384038525	38.0	34.0	40.0	18.0	41.0
45	34.47374266192969	38.0	34.0	40.0	18.0	41.0
46	34.31579006015314	38.0	34.0	40.0	18.0	41.0
47	34.17122752150117	38.0	34.0	40.0	18.0	41.0
48	34.09857575042854	38.0	34.0	40.0	18.0	41.0
49	34.01707465192336	38.0	33.0	40.0	18.0	41.0
50	33.90654813086976	38.0	33.0	40.0	17.0	41.0
51	32.668797438616494	36.0	31.0	40.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	1.0
11	5.0
12	10.0
13	10.0
14	9.0
15	29.0
16	40.0
17	96.0
18	224.0
19	440.0
20	831.0
21	1363.0
22	2206.0
23	3004.0
24	4509.0
25	7535.0
26	11545.0
27	13329.0
28	12598.0
29	11342.0
30	11200.0
31	12578.0
32	15317.0
33	19448.0
34	29563.0
35	36451.0
36	42551.0
37	58793.0
38	104593.0
39	138792.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.152394147000976	18.573187559312778	22.706092757294428	20.568325536391814
2	36.481700556588336	27.147656553238036	22.436248628029247	13.934394262144378
3	25.746992807251807	26.399967314131622	34.34096189310607	13.512077985510503
4	22.966465413336948	21.909931861107346	39.68974425165147	15.433858473904232
5	24.906817417853542	25.9137650220351	32.69645414042666	16.482963419684694
6	21.75894543502848	32.977998324849246	32.362723995698836	12.900332244423437
7	69.20378338926454	4.73685090229711	21.718088099558184	4.341277608880157
8	70.02928728092576	7.543192703622746	17.787240996993273	4.64027901845823
9	64.23311709898061	6.81871785966991	19.215390586841337	9.732774454508144
10	33.37468590923357	28.649349346932635	25.255033345157194	12.720931398676594
11	24.144828111332526	22.539691972833587	36.189384892814495	17.126095023019396
12	21.708988056657983	19.852579305016725	38.336066441456076	20.102366196869216
13	20.807898094376732	21.02945628172247	41.291908947570754	16.870736676330043
14	17.66652614219467	26.50006778603385	36.809859246479306	19.023546825292176
15	16.35147708553483	24.49991549960164	41.47465266621971	17.673954748643812
16	19.103404344620483	25.26691911547583	35.80941167294075	19.820264866962944
17	19.10377577494294	25.778192954338213	35.62926796654898	19.48876330416986
18	18.992346678205767	25.30814788126858	35.969126711597355	19.730378728928294
19	19.14909027428272	26.884683884938315	34.10268934124975	19.86353649952921
20	20.605654283798767	25.757950001764296	36.26422810278962	17.372167611647313
21	20.246481161982622	27.828859764624603	35.2309089457136	16.693750127679174
22	18.909889146620262	23.431867607375864	36.18659916539607	21.471644080607806
23	18.77877424279286	26.81671213592864	35.336209442130226	19.068304179148274
24	19.776064658590535	24.540401404749478	35.04630807545235	20.637225861207632
25	18.84303168857796	29.017622511648984	32.55623919369906	19.583106606074
26	18.68424522572749	25.499248782172828	34.859107192933905	20.957398799165766
27	21.21777145520829	25.51633457700586	33.30727130570759	19.958622662078266
28	19.165990353954527	25.52747748667958	35.55981049624948	19.74672166311641
29	21.607401863465928	23.13398048876516	34.211704140890944	21.046913506877964
30	20.747354951816202	25.2758334432148	35.70708261910377	18.269728985865218
31	23.58025402119753	23.733469029211136	32.407109919232475	20.279167030358856
32	23.042237199118222	25.76110715950518	31.63453484852143	19.56212079285517
33	20.980241763996887	26.66015425501292	31.201818522858748	21.157785458131446
34	24.06868489522879	25.61067787891	31.27053313251334	19.05010409334787
35	20.483639422871565	25.595820666011708	33.39511457696872	20.525425334148004
36	22.91372230754802	28.811850113007676	29.93524112327958	18.33918645616472
37	20.686626094094443	26.945969888143757	33.066398741594064	19.30100527616773
38	20.693497555059903	28.15274700580731	30.42441485795576	20.729340581177023
39	22.39836273513861	27.232342666758285	29.511996270839564	20.857298327263543
40	21.0847993997686	26.90845542557558	33.15089913995309	18.855846034702736
41	20.76109787374712	28.610349163074627	30.16107075933358	20.467482203844675
42	20.19819522006318	26.41259594509517	33.323242809573244	20.065966025268406
43	21.48204412963661	26.854040883335596	30.343071617337625	21.32084336969017
44	21.377115063542444	25.996779699104295	30.56518695016705	22.060918287186208
45	20.324110099376185	24.746730948874472	30.374086049262804	24.55507290248654
46	23.098323177809267	26.362452851563447	29.36862416637107	21.17059980425622
47	19.512534844807124	24.712745074369636	34.17288967219417	21.60183040862907
48	20.321138656796524	24.898645950759484	31.612620459496455	23.16759493294754
49	19.655535518953158	24.099142181670285	34.091360716414805	22.15396158296175
50	19.792036162456196	24.23675711614069	33.17262781381684	22.79857890758628
51	19.61133531058075	23.368353022235677	31.373047901511537	25.64726376567204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	225.0
1	397.5
2	570.0
3	8660.0
4	16750.0
5	11226.0
6	5702.0
7	5622.5
8	5543.0
9	5598.5
10	5654.0
11	5574.5
12	5495.0
13	5437.5
14	5380.0
15	5058.5
16	4737.0
17	4526.0
18	4315.0
19	4198.0
20	4081.0
21	3916.0
22	3751.0
23	3865.5
24	3980.0
25	4302.0
26	5148.0
27	5672.0
28	6187.5
29	6703.0
30	8062.5
31	9422.0
32	11044.5
33	12667.0
34	14053.0
35	15439.0
36	16140.5
37	16842.0
38	17995.5
39	19149.0
40	21016.5
41	22884.0
42	25042.5
43	27201.0
44	30370.0
45	33539.0
46	50959.0
47	68379.0
48	59754.5
49	51130.0
50	49075.5
51	47021.0
52	39707.0
53	32393.0
54	28008.5
55	23624.0
56	21795.0
57	19966.0
58	18104.0
59	16242.0
60	14549.5
61	12857.0
62	11238.5
63	9620.0
64	8046.0
65	6472.0
66	5137.0
67	3802.0
68	3193.0
69	2584.0
70	2094.5
71	1605.0
72	1290.5
73	976.0
74	800.5
75	502.0
76	379.0
77	309.0
78	239.0
79	192.5
80	146.0
81	90.5
82	35.0
83	29.0
84	23.0
85	15.0
86	7.0
87	4.5
88	2.0
89	2.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	538459.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.94896733803083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03290494828285	32.65534751239404
2	8.504987982326721	7.305609021265903
3	3.6753936146570743	4.735630809309413
4	2.2970212208836416	3.9461875756198097
5	1.6708096891653175	3.5879775384013337
6	1.30744795999525	3.369212383800662
7	1.044239213444606	3.139429710992637
8	0.8603119385182613	2.9559607478351038
9	0.7391599421199762	2.857154059052246
>10	3.817519097093916	24.70409032607961
>50	0.028131772228343642	0.8379543683156483
>100	0.014282284360247897	1.124583834918052
>500	0.0017311859830603513	0.6171269970128814
>1k	0.004327964957650878	2.6865471348877032
>5k	0.0012983894872952634	3.4787746232217094
>10k+	4.3279649576508783E-4	1.998413356893219
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10751	1.9966236983688637	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	7938	1.4742069498327635	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	5442	1.010661907406135	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	5335	0.9907903851546728	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	2261	0.41990197953790354	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	2029	0.37681606213286434	TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG	1714	0.3183157863458499	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	1574	0.2923156637738435	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	1437	0.26687268668552294	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	1166	0.2165438779925677	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	1139	0.211529568639395	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1066	0.19797236186970593	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC	1053	0.19555806477373391	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	1014	0.18831517348581786	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	921	0.17104366349155647	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCAGTAT	865	0.1606436144627539	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	832	0.15451501414220953	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTA	702	0.1303720431824893	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8571516122861721E-4	0.0	0.0	0.17085794833032783	0.0
2	1.8571516122861721E-4	0.0	0.0	0.8886470464789334	0.0
3	1.8571516122861721E-4	0.0	0.0	1.4275924443643806	0.0
4	1.8571516122861721E-4	0.0	0.0	2.3327681401926608	0.0
5	1.8571516122861721E-4	0.0	0.0	4.824137028074561	0.0
6	1.8571516122861721E-4	0.0	0.0	6.2794010314620055	0.0
7	1.8571516122861721E-4	0.0	0.0	7.4137492362463995	0.0
8	1.8571516122861721E-4	0.0	0.0	8.938842140255804	0.0
9	1.8571516122861721E-4	0.0	0.0	9.662388408402498	0.0
10	1.8571516122861721E-4	0.0	0.0	11.705069466756058	0.0
11	1.8571516122861721E-4	0.0	0.0	12.688059815139129	0.0
12	1.8571516122861721E-4	0.0	0.0	14.60575456998583	0.0
13	1.8571516122861721E-4	0.0	0.0	15.13634278561599	0.0
14	1.8571516122861721E-4	0.0	0.0	15.437572777128807	0.0
15	1.8571516122861721E-4	0.0	0.0	15.83314607054576	0.0
16	3.7143032245723443E-4	0.0	0.0	16.277562451365842	0.0
17	3.7143032245723443E-4	0.0	0.0	16.79236487829157	0.0
18	3.7143032245723443E-4	0.0	0.0	17.405596340668463	0.0
19	3.7143032245723443E-4	0.0	0.0	17.96515612145029	0.0
20	3.7143032245723443E-4	0.0	0.0	18.39192956195365	0.0
21	3.7143032245723443E-4	0.0	0.0	18.83634594277373	0.0
22	3.7143032245723443E-4	0.0	0.0	19.363591285501773	0.0
23	3.7143032245723443E-4	0.0	0.0	19.818593430511886	0.0
24	3.7143032245723443E-4	0.0	0.0	20.213795293606385	0.0
25	3.7143032245723443E-4	0.0	0.0	20.563125511877413	0.0
26	3.7143032245723443E-4	0.0	0.0	20.90354140240947	0.0
27	3.7143032245723443E-4	0.0	0.0	21.27831459776882	0.0
28	5.571454836858517E-4	0.0	0.0	21.64231631377691	0.0
29	5.571454836858517E-4	0.0	0.0	22.031946722034547	0.0
30	5.571454836858517E-4	0.0	0.0	22.555477761538018	0.0
31	5.571454836858517E-4	0.0	0.0	22.955136788502003	0.0
32	5.571454836858517E-4	0.0	0.0	23.373924477072535	0.0
33	5.571454836858517E-4	0.0	0.0	23.758540575977	0.0
34	5.571454836858517E-4	0.0	0.0	24.13739950488338	0.0
35	5.571454836858517E-4	0.0	0.0	24.52405847056136	0.0
36	5.571454836858517E-4	0.0	0.0	24.924831788492718	0.0
37	5.571454836858517E-4	0.0	0.0	25.310562178364556	0.0
38	5.571454836858517E-4	0.0	0.0	25.717835526938913	0.0
39	5.571454836858517E-4	0.0	0.0	26.15630902259968	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	20	7.0299447E-4	45.0	28
ATATCGA	20	7.0299447E-4	45.0	25
CGAATGC	25	3.8880346E-5	45.0	45
TCTCACG	20	7.0299447E-4	45.0	23
CGGTCTA	20	7.0299447E-4	45.0	31
GCGTAAG	35	1.2103192E-7	45.0	1
CGTTTTT	6060	0.0	44.36881	1
CGTAAGG	70	0.0	41.785713	2
CGTTCTG	105	0.0	40.714287	1
CGAATAT	50	1.0786607E-9	40.5	14
CACAACG	45	1.9250365E-8	40.0	12
CGTTTCT	225	0.0	40.0	1
TACGGCT	765	0.0	39.705883	7
TAGGGCG	165	0.0	39.545452	5
AAGGGCG	115	0.0	39.130436	5
CCGATGA	295	0.0	38.898304	18
GCGATGT	140	0.0	38.571426	9
TTACGAG	35	6.242104E-6	38.571426	1
GTTTTTT	7055	0.0	38.079376	2
CGTATGG	65	9.094947E-12	38.07692	2
>>END_MODULE