##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550718_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125267
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.614383676467067	31.0	31.0	34.0	30.0	34.0
2	31.70554096449983	33.0	31.0	34.0	30.0	34.0
3	31.769340688289812	33.0	31.0	34.0	30.0	34.0
4	35.5429921687276	37.0	35.0	37.0	33.0	37.0
5	35.59110539886802	37.0	35.0	37.0	33.0	37.0
6	35.56624649748138	37.0	35.0	37.0	33.0	37.0
7	35.880519210965375	37.0	35.0	37.0	35.0	37.0
8	35.67651496403682	37.0	35.0	37.0	35.0	37.0
9	37.45034206933989	39.0	37.0	39.0	34.0	39.0
10	36.77698835287825	39.0	37.0	39.0	32.0	39.0
11	36.88767991570006	39.0	37.0	39.0	33.0	39.0
12	37.04711536158765	39.0	37.0	39.0	33.0	39.0
13	37.061029640687494	39.0	37.0	39.0	33.0	39.0
14	38.069723071519235	40.0	37.0	41.0	33.0	41.0
15	38.163363056511294	40.0	37.0	41.0	33.0	41.0
16	38.054715128485554	40.0	37.0	41.0	33.0	41.0
17	38.0732036370313	40.0	37.0	41.0	33.0	41.0
18	37.92751482832669	39.0	37.0	41.0	33.0	41.0
19	37.744745224201104	39.0	37.0	41.0	33.0	41.0
20	37.75570581238475	39.0	36.0	41.0	33.0	41.0
21	37.69148299232839	39.0	36.0	41.0	33.0	41.0
22	37.81948957027789	39.0	36.0	41.0	33.0	41.0
23	37.817421986636546	39.0	36.0	41.0	33.0	41.0
24	37.79834273990756	39.0	36.0	41.0	33.0	41.0
25	37.55724173166117	39.0	35.0	41.0	33.0	41.0
26	37.6570285869383	39.0	36.0	41.0	33.0	41.0
27	37.70405613609331	39.0	36.0	41.0	33.0	41.0
28	37.59207931857552	39.0	36.0	41.0	33.0	41.0
29	37.61439165941549	39.0	36.0	41.0	33.0	41.0
30	37.42583441768383	39.0	35.0	41.0	33.0	41.0
31	37.28544628673154	39.0	35.0	41.0	33.0	41.0
32	37.15990643984449	39.0	35.0	41.0	32.0	41.0
33	36.899191327324836	39.0	35.0	41.0	31.0	41.0
34	36.678678343059225	39.0	35.0	41.0	31.0	41.0
35	36.542537140667534	39.0	35.0	41.0	30.0	41.0
36	36.36426193650363	39.0	35.0	41.0	30.0	41.0
37	36.26090670328179	39.0	35.0	41.0	30.0	41.0
38	36.13753821836557	39.0	35.0	41.0	30.0	41.0
39	36.15942746293916	39.0	35.0	41.0	30.0	41.0
40	36.08832334134289	39.0	35.0	41.0	29.0	41.0
41	35.953219922246085	39.0	35.0	41.0	28.0	41.0
42	35.97789521581901	39.0	35.0	41.0	29.0	41.0
43	35.908826746070396	39.0	35.0	41.0	28.0	41.0
44	35.75859563971357	39.0	35.0	40.0	27.0	41.0
45	35.729282253107364	39.0	35.0	40.0	27.0	41.0
46	35.58693031684322	39.0	35.0	40.0	26.0	41.0
47	35.508146598864826	39.0	35.0	40.0	26.0	41.0
48	35.418059025920634	38.0	35.0	40.0	26.0	41.0
49	35.32454676810333	38.0	35.0	40.0	26.0	41.0
50	35.21776685000838	38.0	34.0	40.0	26.0	41.0
51	34.04092059361204	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	3.0
14	4.0
15	10.0
16	3.0
17	15.0
18	36.0
19	54.0
20	104.0
21	166.0
22	257.0
23	409.0
24	643.0
25	1037.0
26	1632.0
27	1963.0
28	1995.0
29	2066.0
30	2253.0
31	2718.0
32	3356.0
33	4536.0
34	7157.0
35	8692.0
36	10722.0
37	15828.0
38	28183.0
39	31414.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.190960109206735	22.435278245667252	24.70403218724804	15.669729457877974
2	35.6231090390925	24.650546432819496	23.559277383508824	16.16706714457918
3	29.536909162029907	23.148155539767057	31.622853584742987	15.692081713460048
4	26.64628353836206	24.624202703026334	31.127112487726215	17.60240127088539
5	22.75060470834298	28.7769324722393	29.508968842552306	18.963493976865415
6	23.359703672954566	32.552068781083605	29.88736059776318	14.200866948198648
7	73.24993813214972	6.601100050292576	14.683835327739947	5.46512648981775
8	73.50619077650138	5.71179959606281	14.969624881253644	5.812384746182155
9	66.60892333974631	8.354155523801161	17.34455203684929	7.692369099603247
10	31.539032626310203	28.79449495876807	24.570716948597795	15.095755466323931
11	23.72931418490105	23.93846743356191	32.26867411209656	20.063544269440474
12	21.526020420382064	21.14842696001341	36.92432963190625	20.401222987698276
13	21.40547789920729	22.24927554743069	38.87775711081131	17.46748944255071
14	18.70245156346045	25.713076867810358	35.975955359352426	19.608516209376774
15	16.61970032011623	26.16012197945189	37.87030901993342	19.349868680498457
16	18.767113445680028	26.28864744904883	34.962919204579016	19.98131990069212
17	18.572329504179073	26.041176047961557	33.00629854630509	22.38019590155428
18	18.82299408463522	26.259110539886805	35.04514357332737	19.872751802150606
19	19.270837491118968	28.155060790152238	32.8426481036506	19.73145361507819
20	19.08323820319797	28.563787749367354	33.39905960867587	18.95391443875881
21	18.764718561153376	28.742605794023962	34.560578604101636	17.932097040721022
22	17.805168160808513	25.87832390014928	32.57202615213903	23.744481786903172
23	16.951790974478513	27.182737672331896	33.63934635618319	22.226124997006394
24	18.011926524942723	26.04277263764599	33.95786599822779	21.987434839183503
25	18.16040936559509	28.415304908714983	31.06165231066442	22.362633415025506
26	17.33656908842712	28.700296167386462	32.46345805359751	21.499676690588903
27	19.98131990069212	26.351712741583977	32.59198352319446	21.074983834529444
28	16.978932999113894	26.051553880910376	33.938706922014575	23.030806197961155
29	21.10611733337591	23.50579162908028	31.731421683284506	23.656669354259304
30	22.472798103251453	25.450437864720953	30.546752137434442	21.530011894593148
31	20.56966319940607	27.270550104975772	30.165965497696916	21.99382119792124
32	20.594410339514795	26.961610001037783	29.220784404511964	23.223195254935458
33	19.444067471880064	25.398548699976846	30.057397399155406	25.09998642898768
34	18.419855189315623	26.415576328961336	30.787038884941765	24.377529596781276
35	20.634325081625647	26.631115936359933	28.310728284384556	24.42383069762986
36	20.81553801080891	27.551549889436167	30.234618854127582	21.39829324562734
37	18.695266909880495	28.275603311327007	32.25829627914774	20.77083349964476
38	16.466427710410564	28.044896101926287	30.406252245204247	25.08242394245891
39	18.499684673537324	25.735429123392432	30.634564570078314	25.13032163299193
40	19.4552435996711	27.757509958728154	30.15798254927475	22.62926389232599
41	18.189147979914903	29.55766482792755	30.04222979715328	22.21095739500427
42	19.812081394142112	26.928081617664667	33.18910806517279	20.07072892302043
43	20.768438615118107	24.165981463593763	33.08692632536901	21.978653595919116
44	19.333902783654114	24.537986860066894	29.899335020396435	26.228775335882553
45	21.094142910742654	23.690197737632417	28.995665259006763	26.219994092618165
46	21.711224823776416	25.866349477516025	29.139358330605823	23.283067368101733
47	18.73677824167578	25.71467345749479	32.98554288040745	22.563005420421977
48	18.365571140044864	24.728779327356765	32.44749215675318	24.458157375845193
49	19.782544484980082	23.30621791852603	33.295281279187655	23.61595631730623
50	18.423846663526707	22.39616179839862	32.77080156785107	26.4091899702236
51	18.32565639793401	22.244485778377385	30.17714162548796	29.252716198200645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	140.5
2	165.0
3	1244.0
4	2323.0
5	1570.0
6	817.0
7	787.5
8	758.0
9	753.5
10	749.0
11	756.0
12	763.0
13	786.0
14	809.0
15	749.0
16	689.0
17	673.0
18	657.0
19	697.0
20	737.0
21	789.0
22	841.0
23	843.0
24	845.0
25	1017.5
26	1355.0
27	1520.0
28	1759.5
29	1999.0
30	2254.5
31	2510.0
32	2741.0
33	2972.0
34	3083.5
35	3195.0
36	3543.0
37	3891.0
38	4222.0
39	4553.0
40	5020.5
41	5488.0
42	6107.0
43	6726.0
44	7917.0
45	9108.0
46	11141.0
47	13174.0
48	14366.5
49	15559.0
50	14901.0
51	14243.0
52	11616.0
53	8989.0
54	7439.5
55	5890.0
56	4909.0
57	3928.0
58	3464.5
59	3001.0
60	2689.5
61	2378.0
62	1990.0
63	1602.0
64	1402.0
65	1202.0
66	939.5
67	677.0
68	607.5
69	538.0
70	411.5
71	285.0
72	224.5
73	164.0
74	136.5
75	79.5
76	50.0
77	43.5
78	37.0
79	22.0
80	7.0
81	7.5
82	8.0
83	5.5
84	3.0
85	2.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	125267.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.96631195765845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44567214249581	47.640639593827586
2	9.623525652972656	11.54174682877374
3	3.762080992571687	6.767943672315933
4	2.208525253601001	5.2974845729521745
5	1.455044064005964	4.362681312716039
6	0.9398546287174845	3.3815769516313154
7	0.6363321707180702	2.6710945420581638
8	0.46060864240261984	2.2096801232567236
9	0.34345962352565296	1.8536406236279306
>10	1.07963470805932	9.67693007735477
>50	0.02129982161399398	0.8533771863299991
>100	0.022631060464868607	2.6726911317425976
>500	0.0	0.0
>1k	0.0013312388508746238	1.0705133834130298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1341	1.0705133834130298	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	377	0.3009571555158182	No Hit
CTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGCT	370	0.295369091620299	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCC	364	0.2905793225669969	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	282	0.22511914550520087	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTG	242	0.19318735181651991	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC	216	0.17243168591887728	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGC	174	0.13890330254576225	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG	160	0.12772717475472392	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCT	152	0.12134081601698772	No Hit
TCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	145	0.11575275212146853	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC	142	0.11335786759481747	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTT	135	0.10776980369929831	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGC	134	0.10697150885708127	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31053669362242253	0.0
2	0.0	0.0	0.0	1.0936639338373235	0.0
3	0.0	0.0	0.0	1.62852147812273	0.0
4	0.0	0.0	0.0	2.4595464088706525	0.0
5	0.0	0.0	0.0	4.911908164161352	0.0
6	0.0	0.0	0.0	6.675341470618759	0.0
7	0.0	0.0	0.0	8.325416909481348	0.0
8	0.0	0.0	0.0	10.557449288320148	0.0
9	0.0	0.0	0.0	11.520991162876097	0.0
10	0.0	0.0	0.0	13.61012876495805	0.0
11	0.0	0.0	0.0	16.600541243903024	0.0
12	0.0	0.0	0.0	18.90362186369914	0.0
13	0.0	0.0	0.0	19.840820008461925	0.0
14	0.0	0.0	0.0	20.216816879146144	0.0
15	0.0	0.0	0.0	20.869023765237454	0.0
16	0.0	0.0	0.0	22.13032961594035	0.0
17	0.0	0.0	0.0	23.618351201832883	0.0
18	0.0	0.0	0.0	25.10796937740985	0.0
19	0.0	0.0	0.0	26.04516752217264	0.0
20	0.0	0.0	0.0	26.952828757773396	0.0
21	0.0	0.0	0.0	28.080021074983833	0.0
22	0.0	0.0	0.0	29.148139573870214	0.0
23	0.0	0.0	0.0	30.324826171298106	0.0
24	0.0	0.0	0.0	31.22849593268778	0.0
25	0.0	0.0	0.0	32.01321976258711	0.0
26	0.0	0.0	0.0	32.78517087501098	0.0
27	7.982948422170245E-4	0.0	0.0	33.50523282269073	0.0
28	7.982948422170245E-4	0.0	0.0	34.25243679500587	0.0
29	7.982948422170245E-4	0.0	0.0	34.98127998595001	0.0
30	7.982948422170245E-4	0.0	0.0	35.79953219922246	0.0
31	7.982948422170245E-4	0.0	0.0	36.56429865806637	0.0
32	7.982948422170245E-4	0.0	0.0	37.26121005532183	0.0
33	7.982948422170245E-4	0.0	0.0	37.92858454341526	0.0
34	7.982948422170245E-4	0.0	0.0	38.57201018624219	0.0
35	7.982948422170245E-4	0.0	0.0	39.213040944542456	0.0
36	0.001596589684434049	0.0	0.0	39.86285294610712	0.0
37	0.001596589684434049	0.0	0.0	40.515059832198425	0.0
38	0.001596589684434049	0.0	0.0	41.16886330797417	0.0
39	0.0023948845266510733	0.0	0.0	41.77397079837467	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACAAGG	30	2.149989E-6	45.000004	1
ATATGGG	60	0.0	45.000004	3
ATCCTGC	25	3.8708637E-5	45.0	20
CATATGC	25	3.8708637E-5	45.0	33
CGAAAGG	25	3.8708637E-5	45.0	2
GGATGAC	20	7.009212E-4	45.0	8
GGACTCA	25	3.8708637E-5	45.0	8
GGATCCC	20	7.009212E-4	45.0	8
GTCAGAG	20	7.009212E-4	45.0	1
GCGATTA	20	7.009212E-4	45.0	9
TAGGCGG	20	7.009212E-4	45.0	2
AGTAGCA	25	3.8708637E-5	45.0	28
AAGTGCG	25	3.8708637E-5	45.0	1
CGTTTTT	785	0.0	42.993633	1
TAGCGGG	75	0.0	42.000004	3
TAATGGG	65	0.0	41.538464	3
GGTACCT	55	6.002665E-11	40.909092	8
ATAGGGC	50	1.0659278E-9	40.5	4
TGGGGCA	45	1.9048457E-8	40.0	6
AGGGCAC	45	1.9048457E-8	40.0	6
>>END_MODULE