FastQCFastQC Report
Sat 18 Jun 2016
SRR3550714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525291
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.3544701889048166No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC13920.264995973660314No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC12920.2459589065870156No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG12380.23567889036743445No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC7730.14715652847659677No Hit
CCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC6420.12221797061057586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG551.8189894E-1245.0000042
ACGCGAA207.029786E-445.037
GCGAAAC207.029786E-445.031
GTTAGCG253.8879036E-545.01
TCGATTA207.029786E-445.041
CTACGCG207.029786E-445.01
GATATCA207.029786E-445.09
TCGTAAG207.029786E-445.01
CGTAAGG1000.045.02
ATCGGAA207.029786E-445.021
TATAACG207.029786E-445.01
GCGTAAG502.1827873E-1145.01
GCGAAGG950.042.631582
CCCGATC603.6379788E-1241.24999641
CGTTCAT556.002665E-1140.90909217
CCCCGAT556.002665E-1140.90909240
CGATCCC556.002665E-1140.90909243
TCGACAA501.0786607E-940.519
CACGACG451.9248546E-840.00000426
TAGGGCG1250.039.6000025