##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550714_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	525291
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.925469882408038	33.0	31.0	34.0	30.0	34.0
2	31.972845527526648	33.0	31.0	34.0	30.0	34.0
3	32.04721192634178	33.0	31.0	34.0	30.0	34.0
4	35.77082607545151	37.0	35.0	37.0	35.0	37.0
5	35.79143941167848	37.0	35.0	37.0	35.0	37.0
6	35.75307401040566	37.0	35.0	37.0	35.0	37.0
7	36.01489079386474	37.0	35.0	37.0	35.0	37.0
8	35.85147661010754	37.0	35.0	37.0	35.0	37.0
9	37.67029322794413	39.0	38.0	39.0	35.0	39.0
10	36.95571978198751	39.0	37.0	39.0	32.0	39.0
11	36.967878756727224	39.0	37.0	39.0	33.0	39.0
12	37.04748415640093	39.0	37.0	39.0	34.0	39.0
13	37.00529040093967	39.0	37.0	39.0	33.0	39.0
14	38.109171868545246	40.0	37.0	41.0	33.0	41.0
15	38.251142699951075	40.0	37.0	41.0	34.0	41.0
16	38.15526251163641	40.0	37.0	41.0	33.0	41.0
17	38.16831051740844	40.0	37.0	41.0	34.0	41.0
18	38.09180244854757	40.0	37.0	41.0	33.0	41.0
19	38.00731594487627	40.0	37.0	41.0	34.0	41.0
20	38.07575800841819	40.0	37.0	41.0	34.0	41.0
21	38.02682132379957	40.0	37.0	41.0	34.0	41.0
22	38.112600444325146	40.0	37.0	41.0	34.0	41.0
23	38.11916632875872	40.0	37.0	41.0	34.0	41.0
24	38.09259248683111	40.0	37.0	41.0	34.0	41.0
25	37.83933476872819	40.0	36.0	41.0	33.0	41.0
26	37.91377541210491	40.0	36.0	41.0	34.0	41.0
27	37.92986363748855	40.0	36.0	41.0	34.0	41.0
28	37.82366916623357	40.0	36.0	41.0	33.0	41.0
29	37.81379463954266	40.0	36.0	41.0	33.0	41.0
30	37.67650311922344	40.0	36.0	41.0	33.0	41.0
31	37.695363141572955	40.0	36.0	41.0	33.0	41.0
32	37.65161025031839	40.0	36.0	41.0	33.0	41.0
33	37.58332429072647	40.0	36.0	41.0	33.0	41.0
34	37.56782811812881	40.0	36.0	41.0	33.0	41.0
35	37.50469168518021	40.0	36.0	41.0	33.0	41.0
36	37.431299984199235	40.0	36.0	41.0	33.0	41.0
37	37.38142286846719	40.0	36.0	41.0	33.0	41.0
38	37.30900967273378	40.0	36.0	41.0	33.0	41.0
39	37.2695896179451	40.0	36.0	41.0	33.0	41.0
40	37.17261289456701	40.0	35.0	41.0	32.0	41.0
41	37.050933672954606	39.0	35.0	41.0	32.0	41.0
42	37.05796596553149	39.0	35.0	41.0	32.0	41.0
43	37.00339430905917	39.0	35.0	41.0	32.0	41.0
44	36.88502753711752	39.0	35.0	41.0	32.0	41.0
45	36.86010040149174	39.0	35.0	41.0	31.0	41.0
46	36.767113847372215	39.0	35.0	41.0	31.0	41.0
47	36.69937996272542	39.0	35.0	41.0	31.0	41.0
48	36.61707891435414	39.0	35.0	41.0	31.0	41.0
49	36.53266855895114	39.0	35.0	41.0	31.0	41.0
50	36.429975004330934	39.0	35.0	41.0	31.0	41.0
51	35.26965434397315	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	11.0
11	12.0
12	10.0
13	19.0
14	12.0
15	25.0
16	41.0
17	73.0
18	140.0
19	241.0
20	382.0
21	670.0
22	965.0
23	1391.0
24	1914.0
25	2624.0
26	3796.0
27	4572.0
28	5128.0
29	5896.0
30	7147.0
31	9110.0
32	11888.0
33	17169.0
34	30915.0
35	38119.0
36	38932.0
37	59226.0
38	111457.0
39	173326.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93903950381788	20.98341681087245	25.608091514988836	11.469452170320832
2	32.08811877606889	25.474451304134284	25.772571774502133	16.6648581452947
3	31.90783775088475	25.055445457850983	26.901850593290195	16.134866197974077
4	28.825926962388465	25.753534707428834	27.514273041038205	17.906265289144493
5	25.814643692734123	30.73153737642564	25.069532887485224	18.384286043355015
6	26.23003249627349	35.434644796884015	24.050669057722292	14.284653649120202
7	80.19421615828179	6.674395715898426	8.771138283351513	4.36024984246827
8	82.45943676933358	6.276521014066488	7.553146732001881	3.710895484598061
9	76.33197599045101	8.135871355115546	9.285329465001304	6.246823189432144
10	38.62887428111276	30.91810063374396	17.044647633407006	13.408377451736275
11	30.447885077033494	26.964863285302815	24.551534292420772	18.035717345242922
12	28.632510360923753	23.468515546620825	28.336864709275428	19.56210938317999
13	25.257809480840145	24.81424581803229	29.910849414895747	20.01709528623182
14	20.74545347245622	28.902265601352394	28.6252762754359	21.727004650755486
15	20.402215153124647	27.204730330426376	32.66608413241423	19.726970384034754
16	22.549215577651243	25.740208760477522	31.369279123381133	20.341296538490095
17	22.300781852344702	25.749727294014175	28.636888886350615	23.31260196729051
18	22.54141038015119	26.79143560426506	28.57844509043559	22.088708925148158
19	24.717727887970668	27.448785530306058	27.317810508841767	20.515676072881508
20	26.057937409930876	26.47599140286051	27.835428362564752	19.630642824643864
21	25.42400307639004	26.692252484813178	27.800019417808414	20.083725020988368
22	23.239309259058313	26.135608643589936	27.678182188539306	22.946899908812448
23	22.594523797285696	27.264887462377995	28.118890291286164	22.021698449050145
24	22.755387014055067	26.10419748291899	29.067316972877887	22.07309853014805
25	23.02038298771538	27.746715630003177	26.741748859203756	22.491152523077684
26	21.638291918193918	28.832209194522658	27.145144310486952	22.38435457679648
27	22.690089873993653	27.56262719140438	27.87940398750407	21.867878947097893
28	21.30933139916732	28.306595772628885	29.4979354300759	20.886137398127893
29	23.243878155155905	26.877102406094906	28.563405807447683	21.31561363130151
30	22.068720004721193	27.215010346645958	29.226276482939934	21.48999316569292
31	23.92312070833119	27.588136861282603	26.23993177115161	22.248810659234596
32	24.186022604613445	29.10805629641475	26.535577422799932	20.17034367617187
33	23.326689396924753	28.30773799665328	26.917460988290298	21.448111618131662
34	23.295087865583078	27.438886255427942	27.29858307109773	21.967442807891242
35	22.41938278021135	26.92450470310742	27.669996249697785	22.986116266983444
36	23.701529247598	28.072059106285852	27.527408617318784	20.69900302879737
37	22.67143354826182	29.156220076110195	27.794689039027894	20.377657336600095
38	22.62231791521271	28.266427561104223	26.90356392932679	22.207690594356272
39	22.94042730600753	26.729565136276847	27.55672570061166	22.773281857103967
40	23.26919745436339	26.28942814554219	29.351921125623704	21.08945327447072
41	21.290675073435487	27.342368325366323	28.376652179458624	22.99030442173957
42	22.537983708078	27.767085291771608	27.864174333845433	21.830756666304964
43	23.184672876557947	26.821514170240874	27.80611127927187	22.187701673929308
44	22.4098642466747	27.912528484211606	27.41299584420826	22.264611424905432
45	22.19798169014889	26.399462393225846	26.988849989815165	24.413705926810092
46	22.158574961307163	28.18342594866465	27.001985566095744	22.65601352393245
47	22.212640231795326	27.13867170768203	28.603193277630872	22.04549478289177
48	21.924228665634857	26.471612877433653	28.129551048847212	23.47460740808428
49	21.527496187827317	26.16168942548035	29.523635470624853	22.787178916067475
50	20.67273187623622	26.527962595970617	29.100631840256163	23.698673687537003
51	20.649506654406796	26.022909206516008	27.83923577597941	25.488348363097792
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	222.0
1	481.5
2	741.0
3	2020.0
4	3299.0
5	2236.0
6	1173.0
7	1155.5
8	1138.0
9	1181.5
10	1225.0
11	1218.5
12	1212.0
13	1226.5
14	1241.0
15	1147.0
16	1053.0
17	1091.0
18	1129.0
19	1297.0
20	1465.0
21	1691.5
22	1918.0
23	2083.5
24	2249.0
25	2968.5
26	4405.0
27	5122.0
28	6773.0
29	8424.0
30	9264.5
31	10105.0
32	11648.0
33	13191.0
34	15082.0
35	16973.0
36	18042.5
37	19112.0
38	20993.5
39	22875.0
40	24582.5
41	26290.0
42	29139.0
43	31988.0
44	33471.5
45	34955.0
46	39588.5
47	44222.0
48	45131.0
49	46040.0
50	44236.0
51	42432.0
52	37956.0
53	33480.0
54	30356.0
55	27232.0
56	26112.0
57	24992.0
58	24308.0
59	23624.0
60	22654.5
61	21685.0
62	20053.0
63	18421.0
64	15302.5
65	12184.0
66	9828.5
67	7473.0
68	6122.5
69	4772.0
70	3912.0
71	3052.0
72	2534.5
73	2017.0
74	1779.5
75	1133.5
76	725.0
77	512.5
78	300.0
79	234.5
80	169.0
81	121.0
82	73.0
83	52.5
84	32.0
85	26.5
86	21.0
87	12.5
88	4.0
89	4.0
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	525291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.285803370341263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2251158108692	21.444563434075825
2	8.57820061613643	5.024389930310235
3	3.1406240821819864	2.7592709799282047
4	1.6837894680242178	1.9724450911043505
5	1.2331680402590754	1.8057158374808184
6	0.9232491753640631	1.6222856286925005
7	0.7837488215332139	1.6066899715410872
8	0.7077715884448481	1.658212765624794
9	0.6151676844103217	1.6214111860885945
>10	8.940325512131794	54.54768700012891
>50	0.12516265067108454	2.3067760880650443
>100	0.039765217140292484	2.256184706509368
>500	0.0013037776111571306	0.27013888641993195
>1k	0.0026075552223142613	1.1042284940303082
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1862	0.3544701889048166	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC	1392	0.264995973660314	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC	1292	0.2459589065870156	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG	1238	0.23567889036743445	No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	773	0.14715652847659677	No Hit
CCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	642	0.12221797061057586	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.807413414659684E-4	0.0	0.0	0.09118755128109943	0.0
2	3.807413414659684E-4	0.0	0.0	0.4673599966494762	0.0
3	3.807413414659684E-4	0.0	0.0	0.6756255104313609	0.0
4	3.807413414659684E-4	0.0	0.0	0.9642274472625649	0.0
5	3.807413414659684E-4	0.0	0.0	1.831175481780575	0.0
6	3.807413414659684E-4	0.0	0.0	2.350697042210889	0.0
7	3.807413414659684E-4	0.0	0.0	2.8266237190433494	0.0
8	3.807413414659684E-4	0.0	0.0	3.518240365816281	0.0
9	3.807413414659684E-4	0.0	0.0	3.808746009354815	0.0
10	3.807413414659684E-4	0.0	0.0	4.628101376189579	0.0
11	3.807413414659684E-4	0.0	0.0	5.398531480645966	0.0
12	3.807413414659684E-4	0.0	0.0	6.267763963212771	0.0
13	3.807413414659684E-4	0.0	0.0	6.567978510958688	0.0
14	3.807413414659684E-4	0.0	0.0	6.701999463154708	0.0
15	3.807413414659684E-4	0.0	0.0	6.928921302668425	0.0
16	3.807413414659684E-4	0.0	0.0	7.341835287488268	0.0
17	3.807413414659684E-4	0.0	0.0	7.846698306272143	0.0
18	3.807413414659684E-4	0.0	0.0	8.412479939690572	0.0
19	3.807413414659684E-4	0.0	0.0	8.790936833107745	0.0
20	5.711120121989526E-4	0.0	0.0	9.135888488475912	0.0
21	5.711120121989526E-4	0.0	0.0	9.536238009027377	0.0
22	5.711120121989526E-4	0.0	0.0	9.96761794890832	0.0
23	5.711120121989526E-4	0.0	0.0	10.403566784886854	0.0
24	5.711120121989526E-4	0.0	0.0	10.763938464584392	0.0
25	5.711120121989526E-4	0.0	0.0	11.096706397025649	0.0
26	5.711120121989526E-4	0.0	0.0	11.4138639344668	0.0
27	5.711120121989526E-4	0.0	0.0	11.748535573615387	0.0
28	5.711120121989526E-4	0.0	0.0	12.089679815568894	0.0
29	5.711120121989526E-4	0.0	0.0	12.431775910876066	0.0
30	7.614826829319368E-4	0.0	0.0	12.854208429232559	0.0
31	7.614826829319368E-4	0.0	0.0	13.199160084600726	0.0
32	7.614826829319368E-4	0.0	0.0	13.554011014847008	0.0
33	7.614826829319368E-4	0.0	0.0	13.89477451545905	0.0
34	7.614826829319368E-4	0.0	0.0	14.262951392656642	0.0
35	7.614826829319368E-4	0.0	0.0	14.653401638329992	0.0
36	9.518533536649209E-4	0.0	0.0	15.028241489003237	0.0
37	9.518533536649209E-4	0.0	0.0	15.381950195225123	0.0
38	9.518533536649209E-4	0.0	0.0	15.736610754800672	0.0
39	9.518533536649209E-4	0.0	0.0	16.12820322449842	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	55	1.8189894E-12	45.000004	2
ACGCGAA	20	7.029786E-4	45.0	37
GCGAAAC	20	7.029786E-4	45.0	31
GTTAGCG	25	3.8879036E-5	45.0	1
TCGATTA	20	7.029786E-4	45.0	41
CTACGCG	20	7.029786E-4	45.0	1
GATATCA	20	7.029786E-4	45.0	9
TCGTAAG	20	7.029786E-4	45.0	1
CGTAAGG	100	0.0	45.0	2
ATCGGAA	20	7.029786E-4	45.0	21
TATAACG	20	7.029786E-4	45.0	1
GCGTAAG	50	2.1827873E-11	45.0	1
GCGAAGG	95	0.0	42.63158	2
CCCGATC	60	3.6379788E-12	41.249996	41
CGTTCAT	55	6.002665E-11	40.909092	17
CCCCGAT	55	6.002665E-11	40.909092	40
CGATCCC	55	6.002665E-11	40.909092	43
TCGACAA	50	1.0786607E-9	40.5	19
CACGACG	45	1.9248546E-8	40.000004	26
TAGGGCG	125	0.0	39.600002	5
>>END_MODULE