FastQCFastQC Report
Sat 18 Jun 2016
SRR3550713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554467
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81451.468978316112591No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC63391.1432601038474788No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCG56411.0173734415213167No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC51060.9208843808558489No Hit
GCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC23890.43086423538280905No Hit
GAACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT16550.29848485121747553No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTT14640.26403735479298135No Hit
CGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTG14190.25592145249401677No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCT11040.19911013640126463No Hit
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT10010.1805337378058568No Hit
CCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGC8990.16213769259487038No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTC8490.15312002337379862No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTT8430.15203790306727002No Hit
CGTCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT7600.13706857216029086No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAACGACGT7490.1350846849316551No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAACGACGTCGTA6970.12570630894174042No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC6460.11650828633624724No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCT6380.11506545926087575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG302.1634605E-645.00000440
GCTACGA302.1634605E-645.00000410
CCACGAA351.2104101E-745.00000425
TGCGTAG302.1634605E-645.0000041
CGGGTCC406.8048394E-945.06
CGAACGG207.0301234E-445.02
GACCGAC253.8881844E-545.09
CCGTCGA207.0301234E-445.041
CGTAGAT207.0301234E-445.037
CGGGCTA253.8881844E-545.06
CAAGCGT207.0301234E-445.038
GATCTCG207.0301234E-445.09
CGTCATA502.1827873E-1145.038
TCGCGTC253.8881844E-545.040
CCGCAGG406.8048394E-945.02
CGTTTTT51750.044.1739121
CGGCGAA700.041.78571731
GCATGCG650.041.5384641
AACGAGC1150.041.0869615
TCTTGCG556.002665E-1140.9090921