##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550709_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478943
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.373944289821544	33.0	31.0	34.0	30.0	34.0
2	32.520412658708864	34.0	31.0	34.0	31.0	34.0
3	32.497274205907594	34.0	31.0	34.0	31.0	34.0
4	35.99697249985906	37.0	35.0	37.0	35.0	37.0
5	36.06666137724113	37.0	35.0	37.0	35.0	37.0
6	36.10526304800362	37.0	35.0	37.0	35.0	37.0
7	36.09385041643787	37.0	36.0	37.0	35.0	37.0
8	36.00064934658195	37.0	36.0	37.0	35.0	37.0
9	37.848188615346714	39.0	38.0	39.0	35.0	39.0
10	37.64770546808284	39.0	37.0	39.0	35.0	39.0
11	37.5884186635988	39.0	37.0	39.0	35.0	39.0
12	37.48781796581221	39.0	37.0	39.0	35.0	39.0
13	37.470471433970225	39.0	37.0	39.0	35.0	39.0
14	38.75523600929547	40.0	38.0	41.0	35.0	41.0
15	38.843568023752304	40.0	38.0	41.0	35.0	41.0
16	38.881248916885724	40.0	38.0	41.0	35.0	41.0
17	38.83244770254498	40.0	38.0	41.0	35.0	41.0
18	38.78267768815913	40.0	38.0	41.0	35.0	41.0
19	38.73064853228881	40.0	38.0	41.0	35.0	41.0
20	38.6578924840743	40.0	38.0	41.0	35.0	41.0
21	38.557133103521714	40.0	38.0	41.0	35.0	41.0
22	38.53120726265965	40.0	38.0	41.0	35.0	41.0
23	38.48947995899303	40.0	38.0	41.0	35.0	41.0
24	38.45883121791111	40.0	37.0	41.0	34.0	41.0
25	38.35542016482128	40.0	37.0	41.0	34.0	41.0
26	38.35727007180395	40.0	37.0	41.0	34.0	41.0
27	38.32380471162539	40.0	37.0	41.0	34.0	41.0
28	38.23933328183103	40.0	37.0	41.0	34.0	41.0
29	38.18489674136588	40.0	37.0	41.0	34.0	41.0
30	38.106265254946834	40.0	37.0	41.0	34.0	41.0
31	38.022033937232614	40.0	37.0	41.0	34.0	41.0
32	37.92263797570901	40.0	37.0	41.0	34.0	41.0
33	37.861315438371584	40.0	37.0	41.0	34.0	41.0
34	37.75090981599063	40.0	36.0	41.0	33.0	41.0
35	37.66795422419787	40.0	36.0	41.0	33.0	41.0
36	37.59350068797331	40.0	36.0	41.0	33.0	41.0
37	37.51454348429771	40.0	36.0	41.0	33.0	41.0
38	37.45216654173879	40.0	36.0	41.0	33.0	41.0
39	37.368810067168745	40.0	35.0	41.0	33.0	41.0
40	37.288714941026385	40.0	35.0	41.0	33.0	41.0
41	37.20527912507334	40.0	35.0	41.0	33.0	41.0
42	37.1248624575367	40.0	35.0	41.0	33.0	41.0
43	37.072937698222965	39.0	35.0	41.0	32.0	41.0
44	36.984900082055695	39.0	35.0	41.0	32.0	41.0
45	36.870915745715045	39.0	35.0	41.0	32.0	41.0
46	36.78858862119292	39.0	35.0	41.0	32.0	41.0
47	36.69780119972523	39.0	35.0	41.0	31.0	41.0
48	36.64980801473244	39.0	35.0	41.0	31.0	41.0
49	36.6096278680344	39.0	35.0	41.0	31.0	41.0
50	36.50412679588177	39.0	35.0	41.0	31.0	41.0
51	35.583075647832835	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	14.0
10	10.0
11	7.0
12	13.0
13	12.0
14	7.0
15	10.0
16	25.0
17	56.0
18	99.0
19	135.0
20	259.0
21	424.0
22	652.0
23	1098.0
24	1763.0
25	2269.0
26	3070.0
27	3561.0
28	4047.0
29	4530.0
30	5590.0
31	7058.0
32	8895.0
33	12593.0
34	24370.0
35	35277.0
36	31883.0
37	47967.0
38	88243.0
39	194729.0
40	272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3069133487701	21.936222055651715	23.989911116771726	11.766953478806455
2	31.822784757267563	25.525375671008867	24.754720290305947	17.897119281417623
3	32.436010130641854	24.489135450356304	26.160733114378953	16.91412130462289
4	29.544434306378836	26.000588796579134	25.463990495737487	18.99098640130454
5	24.795226154260526	31.135020242492324	24.03542801544234	20.034325587804812
6	25.96321482932207	35.2465742269957	23.803876452939075	14.986334490743157
7	78.81376280684758	6.9029091144457695	8.904608690386956	5.378719388319696
8	78.43396813399507	6.724599795800336	9.296513363803207	5.544918706401388
9	71.27257314544737	9.254128361830114	12.069077113560486	7.4042213791620295
10	40.023551863165345	25.529969119498563	18.616829142507562	15.829649874828528
11	30.00190001732983	25.039305303553867	24.612740973351734	20.346053705764568
12	27.13913764268399	22.684118987019332	29.238552395587785	20.93819097470889
13	24.711500115880177	23.475444885925885	30.369793482731765	21.443261515462176
14	20.521857507051987	27.085686605713	29.494950338558034	22.897505548676982
15	20.753617862668417	25.42097911442489	32.68175962484053	21.14364339806616
16	22.80480140643041	25.308857212653695	30.54079504241632	21.34554633849957
17	21.624911523918293	26.37077898622592	28.425094426685433	23.579215063170356
18	22.650920882025627	25.734795163516328	29.41769688668589	22.196587067772157
19	24.289738027280908	26.946839185456305	26.80047521312557	21.962947574137214
20	25.303219798598164	26.824486421139888	26.905915735275386	20.966378044986563
21	24.088461466187	27.420590759234397	27.962200094792074	20.52874767978653
22	23.049298141950086	25.00944788837085	27.02200470619677	24.919249263482293
23	23.018605554314398	26.215019323802625	27.300325926049656	23.466049195833325
24	22.878922961605035	25.47505653073539	27.98788164771173	23.658138859947844
25	22.689965194188037	26.97105918658379	26.40919691904882	23.92977870017935
26	21.717824459278035	28.347840974813288	26.747232969267742	23.187101596640936
27	23.663149894663874	27.081719536562805	26.803815902936257	22.451314665837064
28	21.36726082226904	27.620614561649298	27.2752707524695	23.73685386361216
29	22.531700014406724	26.861860388396945	26.200821392107205	24.405618205089123
30	23.13532090457528	28.032354580816506	25.102987203070093	23.729337311538114
31	23.852942834533547	28.25952148794324	23.582555752981044	24.30497992454217
32	23.41238936574916	28.397324942634093	23.657303687495173	24.532982004121575
33	22.88873623792393	27.770945603130226	24.084076810810473	25.256241348135372
34	21.99510171356508	26.83617883547729	25.208845311446247	25.95987413951138
35	21.59213100515093	27.378205757261302	24.78478649860213	26.24487673898564
36	21.81199015331678	28.84184548056867	24.855358570852896	24.49080579526165
37	22.51979880695615	27.820429570951035	26.005391038181997	23.654380583910818
38	21.90427670933702	27.329974548119505	25.440814460175847	25.32493428236763
39	22.432314492538776	24.333166994819845	27.170665402772354	26.063853109869022
40	21.89717774348931	25.309901178219533	28.16493820767816	24.627982870612996
41	20.071073175722372	26.967509703659935	27.147906953437044	25.81351016718065
42	21.75728635766678	26.696913828994262	27.68408766805236	23.8617121452866
43	22.665327606834214	24.263847681248084	29.093441181936054	23.977383529981648
44	22.562183808929245	24.982095990545847	26.366394330849392	26.089325869675516
45	22.073399131003065	24.433805275366797	26.525494683083373	26.967300910546765
46	22.750306403893575	25.634783262308876	26.566209340151126	25.048700993646428
47	21.19145702098162	25.58947515675143	28.526776672798228	24.692291149468726
48	20.71457355050601	24.808380120390108	28.77440530501542	25.702641024088464
49	22.139795340990474	24.13126405438643	28.767097546054543	24.961843058568554
50	20.25314077040483	23.854821972552056	28.790064788503017	27.101972468540097
51	20.683880962870322	23.406543158580455	27.223490060403847	28.686085818145372
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	262.0
1	531.0
2	800.0
3	1736.0
4	2672.0
5	1869.5
6	1067.0
7	1112.0
8	1157.0
9	1167.5
10	1178.0
11	1244.5
12	1311.0
13	1295.5
14	1280.0
15	1259.0
16	1238.0
17	1219.5
18	1201.0
19	1272.5
20	1344.0
21	1484.5
22	1625.0
23	1663.5
24	1702.0
25	2229.5
26	3352.0
27	3947.0
28	4525.5
29	5104.0
30	5988.5
31	6873.0
32	7781.5
33	8690.0
34	9764.0
35	10838.0
36	11668.5
37	12499.0
38	13458.0
39	14417.0
40	16752.5
41	19088.0
42	21294.0
43	23500.0
44	26477.0
45	29454.0
46	35240.0
47	41026.0
48	45173.5
49	49321.0
50	48585.5
51	47850.0
52	41359.5
53	34869.0
54	31265.0
55	27661.0
56	25714.5
57	23768.0
58	22914.0
59	22060.0
60	21235.0
61	20410.0
62	18622.5
63	16835.0
64	14408.0
65	11981.0
66	10354.0
67	8727.0
68	7849.0
69	6971.0
70	5897.0
71	4823.0
72	4212.0
73	3601.0
74	3041.5
75	1864.0
76	1246.0
77	960.0
78	674.0
79	527.5
80	381.0
81	261.0
82	141.0
83	99.5
84	58.0
85	45.0
86	32.0
87	22.0
88	12.0
89	8.5
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	478943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.20193924369125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.40823227987822	25.13708254215665
2	9.255132731514403	6.515972402141365
3	3.4647815165665397	3.6590108531651926
4	2.0713015234367314	2.9165532153353984
5	1.5650339511510478	2.7546115031366623
6	1.3777208662248228	2.9099067736567137
7	1.1917676439281844	2.936677253590991
8	1.09602345297065	3.0865720800906815
9	1.0257252383318471	3.2496765768430564
>10	7.435395593422944	40.92571680997107
>50	0.06485512065924157	1.588273971338076
>100	0.03927007305972424	2.6935517963085394
>500	0.0029750055348275943	0.720724084844299
>1k	0.0017850033208965566	0.9056701374213161
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT	1521	0.31757432512845996	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1476	0.308178635035902	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	1327	0.2770684611738766	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCC	898	0.18749621562482383	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	811	0.16933121477921173	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	694	0.14490242053856095	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	532	0.11107793620535221	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	506	0.10564931526298535	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010439655658397763	0.0	0.0	0.32884915323952957	0.0
2	0.0010439655658397763	0.0	0.0	0.9648329759491213	0.0
3	0.0010439655658397763	0.0	0.0	1.469903516702405	0.0
4	0.0010439655658397763	0.0	0.0	2.071645268852452	0.0
5	0.0010439655658397763	0.0	0.0	3.551988441213255	0.0
6	0.0010439655658397763	0.0	0.0	4.671954700246167	0.0
7	0.0010439655658397763	0.0	0.0	5.653908711475061	0.0
8	0.0010439655658397763	0.0	0.0	7.35724292869924	0.0
9	0.0010439655658397763	0.0	0.0	8.146689689587278	0.0
10	0.0012527586790077316	0.0	0.0	9.435778370286235	0.0
11	0.001461551792175687	0.0	0.0	11.056012928469567	0.0
12	0.001461551792175687	0.0	0.0	12.31378264219333	0.0
13	0.001461551792175687	0.0	0.0	12.871886633691274	0.0
14	0.001461551792175687	0.0	0.0	13.123691128171828	0.0
15	0.001461551792175687	0.0	0.0	13.483859248386551	0.0
16	0.001461551792175687	0.0	0.0	14.1292387611887	0.0
17	0.001461551792175687	0.0	0.0	14.905322762833991	0.0
18	0.001461551792175687	0.0	0.0	15.729846766734246	0.0
19	0.001461551792175687	0.0	0.0	16.285027654647838	0.0
20	0.001461551792175687	0.0	0.0	16.81765888633929	0.0
21	0.001461551792175687	0.0	0.0	17.451345984804036	0.0
22	0.001461551792175687	0.0	0.0	18.136396189108098	0.0
23	0.001461551792175687	0.0	0.0	18.80891880662208	0.0
24	0.001461551792175687	0.0	0.0	19.338418141616017	0.0
25	0.001461551792175687	0.0	0.0	19.82427971595785	0.0027143104711834184
26	0.001461551792175687	0.0	0.0	20.262745253610554	0.0027143104711834184
27	0.001461551792175687	0.0	0.0	20.691606308057533	0.0027143104711834184
28	0.001461551792175687	0.0	0.0	21.146984087876845	0.0027143104711834184
29	0.001461551792175687	0.0	0.0	21.60090031590398	0.0027143104711834184
30	0.001461551792175687	0.0	0.0	22.113278615618142	0.0027143104711834184
31	0.001461551792175687	0.0	0.0	22.597052258828295	0.0027143104711834184
32	0.001670344905343642	0.0	0.0	23.06119934940066	0.0027143104711834184
33	0.001670344905343642	0.0	0.0	23.538709199215774	0.0027143104711834184
34	0.0018791380185115975	0.0	0.0	23.99951559997745	0.0027143104711834184
35	0.0018791380185115975	0.0	0.0	24.450508724420235	0.0027143104711834184
36	0.0018791380185115975	0.0	0.0	24.90922719405023	0.0027143104711834184
37	0.0018791380185115975	0.0	0.0	25.370659974151412	0.0027143104711834184
38	0.0018791380185115975	0.0	0.0	25.833136719818434	0.0027143104711834184
39	0.0018791380185115975	0.0	0.0	26.307097086709692	0.0027143104711834184
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.029159E-4	45.000004	21
GTTAACG	20	7.029159E-4	45.000004	1
ACGTAAG	25	3.887385E-5	45.000004	1
AATACGG	25	3.887385E-5	45.000004	2
GCTACGA	90	0.0	42.500004	10
GCGATTA	55	6.002665E-11	40.909092	9
CGTTTTT	915	0.0	40.32787	1
CAATCGG	35	6.24066E-6	38.571426	2
CTACGAA	100	0.0	38.250004	11
TCGATCA	65	9.094947E-12	38.076927	17
AAGGGTA	130	0.0	38.076927	5
TGCGTGG	65	9.094947E-12	38.076927	2
AGCGACG	30	1.1389276E-4	37.500004	1
ACGTAGG	30	1.1389276E-4	37.500004	2
CACAACG	30	1.1389276E-4	37.500004	12
GGCGATT	205	0.0	37.317074	8
GGGCGAT	750	0.0	36.9	7
TATACTG	105	0.0	36.42857	18
ATTTACG	25	0.0021058838	36.000004	1
TTTCGAT	25	0.0021058838	36.000004	28
>>END_MODULE