FastQCFastQC Report
Sat 18 Jun 2016
SRR3550704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504009
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46200.9166502979113468No Hit
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT17290.3430494296728828Illumina Single End Adapter 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC14450.286701229541536Illumina Single End Adapter 1 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT12640.2507891724155719No Hit
CGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTG12400.24602735268616235Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT11630.23074984772097326No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC11510.22836893785626844No Hit
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC11380.22578961883617157Illumina Single End Adapter 1 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC8310.1648780081308072No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTC6440.12777549607249078No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT5960.11825185661367159Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC5280.10476003404701108Illumina Single End Adapter 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG453.8380676E-1045.0000041
TCGTTAG207.029514E-445.01
TAAACGG253.8876773E-545.02
GTCGTAG207.029514E-445.01
CGAATAT551.8189894E-1245.014
TTGCCGT207.029514E-445.026
TTCGCAA207.029514E-445.014
AACACGG207.029514E-445.02
CGTTTTT33200.043.644581
CGACAGG750.042.02
GCTACGA603.6379788E-1241.24999610
GCGACAG501.0786607E-940.51
TATACGG501.0786607E-940.52
CGTTTTC1400.040.178571
CGCGAGG850.039.7058832
TTTACGG403.4530785E-739.3752
CGTTCTG800.039.3751
ACGGGTC403.4530785E-739.3755
TTGGTCG403.4530785E-739.3751
CGTAAGG750.039.02
Produced by FastQC (version 0.11.5)