##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550704_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504009
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30625246771387	33.0	31.0	34.0	30.0	34.0
2	32.46962851853836	34.0	31.0	34.0	31.0	34.0
3	32.38992160854271	34.0	31.0	34.0	30.0	34.0
4	35.93666184532419	37.0	35.0	37.0	35.0	37.0
5	36.0336759859447	37.0	35.0	37.0	35.0	37.0
6	36.090926947733074	37.0	35.0	37.0	35.0	37.0
7	36.1573860784232	37.0	36.0	37.0	35.0	37.0
8	36.10424417024299	37.0	37.0	37.0	35.0	37.0
9	37.95938167770814	39.0	38.0	39.0	35.0	39.0
10	37.68867619427431	39.0	37.0	39.0	35.0	39.0
11	37.617850078074	39.0	37.0	39.0	35.0	39.0
12	37.58700737486831	39.0	37.0	39.0	35.0	39.0
13	37.606179651553845	39.0	37.0	39.0	35.0	39.0
14	38.9094143160142	40.0	38.0	41.0	35.0	41.0
15	38.97632185139551	40.0	38.0	41.0	35.0	41.0
16	39.01015259648141	40.0	38.0	41.0	35.0	41.0
17	38.97802618604033	40.0	38.0	41.0	35.0	41.0
18	38.84214964415318	40.0	38.0	41.0	35.0	41.0
19	38.72206051876058	40.0	38.0	41.0	35.0	41.0
20	38.59149142178017	40.0	37.0	41.0	35.0	41.0
21	38.51340948276717	40.0	37.0	41.0	35.0	41.0
22	38.49209240311185	40.0	37.0	41.0	35.0	41.0
23	38.47342408568101	40.0	37.0	41.0	35.0	41.0
24	38.45817038981447	40.0	37.0	41.0	35.0	41.0
25	38.38142771260037	40.0	37.0	41.0	35.0	41.0
26	38.40573481822745	40.0	37.0	41.0	35.0	41.0
27	38.38520343882748	40.0	37.0	41.0	35.0	41.0
28	38.32594259229498	40.0	37.0	41.0	35.0	41.0
29	38.28309613518806	40.0	37.0	41.0	34.0	41.0
30	38.16662400869826	40.0	37.0	41.0	34.0	41.0
31	38.02613842213135	40.0	37.0	41.0	34.0	41.0
32	37.80523760488404	40.0	37.0	41.0	33.0	41.0
33	37.563489937679684	40.0	36.0	41.0	33.0	41.0
34	37.28385008997855	40.0	36.0	41.0	33.0	41.0
35	37.1071072143553	40.0	36.0	41.0	32.0	41.0
36	37.000807525262445	40.0	36.0	41.0	31.0	41.0
37	36.84875865311929	40.0	35.0	41.0	31.0	41.0
38	36.76756169036664	40.0	35.0	41.0	31.0	41.0
39	36.67970413226748	40.0	35.0	41.0	31.0	41.0
40	36.566237904481866	40.0	35.0	41.0	30.0	41.0
41	36.54482757252351	39.0	35.0	41.0	30.0	41.0
42	36.47799940080435	39.0	35.0	41.0	30.0	41.0
43	36.38509431379202	39.0	35.0	41.0	30.0	41.0
44	36.3051274878028	39.0	35.0	41.0	30.0	41.0
45	36.24843207164952	39.0	35.0	41.0	30.0	41.0
46	36.207700656139075	39.0	35.0	41.0	30.0	41.0
47	36.150326680674354	39.0	35.0	41.0	30.0	41.0
48	36.08997656787875	39.0	35.0	41.0	29.0	41.0
49	36.01206327664784	39.0	35.0	41.0	29.0	41.0
50	35.915602697570876	39.0	35.0	41.0	28.0	41.0
51	34.967522405353876	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	14.0
11	10.0
12	5.0
13	7.0
14	8.0
15	14.0
16	24.0
17	34.0
18	90.0
19	156.0
20	275.0
21	429.0
22	737.0
23	1207.0
24	2286.0
25	4231.0
26	6089.0
27	6869.0
28	6492.0
29	6182.0
30	6528.0
31	7513.0
32	9349.0
33	12393.0
34	23012.0
35	32330.0
36	31155.0
37	47864.0
38	93842.0
39	204598.0
40	258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.1909846847973	21.544456547402923	24.67634506526669	15.588213702533091
2	35.887851209006186	23.534103557674566	24.517220922642256	16.060824310676992
3	29.692326922733525	22.746419210768064	31.513326150921905	16.047927715576506
4	26.762220515903483	24.414444980149163	31.051032818858392	17.772301685088955
5	23.396606012987863	28.29810578779347	29.640740542331585	18.66454765688708
6	24.199369455704165	32.286328220329395	28.842540510189302	14.671761813777135
7	76.34744617655636	5.211613284683408	13.289246819005216	5.151693719755005
8	77.05120345073203	4.469364634361688	13.212859294179271	5.26657262072701
9	70.80806096716526	7.118325268001166	15.55269846371791	6.5209153011156555
10	35.20532371445748	28.584807017335006	22.014289427371338	14.195579840836176
11	25.132686122668446	23.845010704173934	31.796257606511	19.226045566646626
12	23.1281584257424	22.114684459999722	34.43609141900244	20.32106569525544
13	22.209127217966344	23.187879581515407	35.611070437234254	18.991922763283988
14	19.138348719963332	26.458456098998234	33.44642655190681	20.956768629131624
15	17.934203555888885	26.11620030594692	36.61660803676125	19.33298810140295
16	21.001410689094836	26.453694279268824	33.092067800376576	19.452827231259757
17	20.839707227450305	26.052510967066066	31.4184865746445	21.689295230839132
18	20.783755845629738	26.21361920124442	32.51529238565185	20.48733256747399
19	21.152003238037416	27.634823981317798	30.86155207545897	20.35162070518582
20	21.825007093127304	27.763591523167246	30.861750484614365	19.549650899091088
21	21.127797321079584	27.73740151465549	32.185139551079445	18.94966161318548
22	20.32086728610005	25.408474848663413	31.11392852111768	23.156729344118855
23	19.615125920370467	26.307069913434084	31.799828971308052	22.277975194887393
24	20.36491411859709	25.43863304028301	32.31351027461811	21.88294256650179
25	20.03178514669381	27.502881892982067	29.923076770454493	22.542256189869626
26	19.49310428980435	27.10646040050872	31.422851576063128	21.977583733623803
27	20.53614121970044	26.055090286086163	31.81312238471932	21.595646109494076
28	19.186165326412823	26.016995728250887	32.03335654720452	22.763482398131778
29	20.255987492286845	24.80293010640683	32.25438434631128	22.68669805499505
30	22.274403830090336	25.076337922537096	32.01589654153001	20.633361705842553
31	22.52618504828287	26.448138822917844	29.833991059683456	21.191685069115827
32	22.648603497159773	27.732441285770694	29.60820144084729	20.010753776222252
33	23.658109279794605	26.87035350559216	29.0302355711902	20.441301643423035
34	22.297419292115816	27.071341980004327	29.824070601913853	20.807168125966005
35	22.641659176721053	26.92769375150047	29.899069262652056	20.531577809126425
36	24.877135130523463	27.152689733715075	28.781033672017763	19.189141463743702
37	23.844217067552364	28.333819435764045	28.87210347434272	18.94986002234087
38	24.53706183818146	27.816169949346143	27.643950802465827	20.002817410006568
39	25.094988383143953	26.735236870770162	27.471334837274732	20.698439908811153
40	24.05988781946354	26.10707348479888	29.82764196671091	20.005396729026664
41	22.622215079492626	25.97552821477394	28.942538724506907	22.459717981226525
42	23.98290506717142	25.737437228303463	29.269517012593028	21.01014069193209
43	23.540055832336325	24.45928544926777	30.566319252235573	21.434339466160328
44	22.70614215222347	25.38863393312421	28.438777878966448	23.46644603568587
45	22.717848292391604	24.42734157524965	29.059401717032827	23.795408415325916
46	23.610491082500513	25.159669767801766	28.72488388104181	22.504955268655916
47	21.54624222980145	25.23327956445222	30.831989111305553	22.388489094440775
48	21.973813959671354	24.320399040493324	30.94468551156825	22.761101488267073
49	23.515849915378496	23.112285693311033	30.86155207545897	22.510312315851504
50	21.0073629637566	23.543031969667208	31.34428155052787	24.105323516048323
51	21.21569257691827	22.87697243501604	29.470703896160583	26.436631091905106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	309.0
1	432.0
2	555.0
3	4177.0
4	7799.0
5	5330.0
6	2861.0
7	2864.5
8	2868.0
9	2850.5
10	2833.0
11	2895.0
12	2957.0
13	2915.0
14	2873.0
15	2764.0
16	2655.0
17	2493.0
18	2331.0
19	2328.0
20	2325.0
21	2466.0
22	2607.0
23	2648.5
24	2690.0
25	2965.5
26	3662.0
27	4083.0
28	4893.0
29	5703.0
30	6462.0
31	7221.0
32	8312.0
33	9403.0
34	10452.0
35	11501.0
36	13489.0
37	15477.0
38	16768.0
39	18059.0
40	20294.0
41	22529.0
42	25637.0
43	28745.0
44	31990.5
45	35236.0
46	42584.5
47	49933.0
48	54684.5
49	59436.0
50	56833.5
51	54231.0
52	45666.0
53	37101.0
54	31365.0
55	25629.0
56	22582.5
57	19536.0
58	17854.5
59	16173.0
60	14965.5
61	13758.0
62	12143.0
63	10528.0
64	8813.0
65	7098.0
66	5969.5
67	4841.0
68	4096.5
69	3352.0
70	2839.0
71	2326.0
72	1867.5
73	1409.0
74	1137.0
75	677.5
76	490.0
77	384.5
78	279.0
79	187.0
80	95.0
81	66.5
82	38.0
83	22.0
84	6.0
85	11.0
86	16.0
87	11.0
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.71950450983579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75485525688119	33.430000867922196
2	9.686009483935647	8.663070895983447
3	3.686721898233784	4.946051296637282
4	2.2883763757855147	4.09340230628568
5	1.667310731897364	3.728065489719088
6	1.2789611946535082	3.431670654732751
7	1.0517318027517775	3.292304756740709
8	0.902708119471214	3.2294927855806668
9	0.7947599872924401	3.1987145552365175
>10	3.812049652308832	25.12883582742938
>50	0.044485753948538924	1.3838133361508178
>100	0.02669145236681301	2.2221384364288292
>500	0.001779430157787534	0.517040089192348
>1k	0.003558860315575068	2.735398701960287
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4620	0.9166502979113468	No Hit
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT	1729	0.3430494296728828	Illumina Single End Adapter 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	1445	0.286701229541536	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT	1264	0.2507891724155719	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTG	1240	0.24602735268616235	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT	1163	0.23074984772097326	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC	1151	0.22836893785626844	No Hit
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	1138	0.22578961883617157	Illumina Single End Adapter 1 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC	831	0.1648780081308072	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTC	644	0.12777549607249078	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT	596	0.11825185661367159	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	528	0.10476003404701108	Illumina Single End Adapter 1 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35654125223954336	0.0
2	0.0	0.0	0.0	1.09363126452107	0.0
3	0.0	0.0	0.0	1.8086978605540773	0.0
4	0.0	0.0	0.0	2.633087901208113	0.0
5	0.0	0.0	0.0	4.4441666716268955	0.0
6	0.0	0.0	0.0	6.024297185169313	0.0
7	0.0	0.0	0.0	7.43835923564857	0.0
8	0.0	0.0	0.0	9.439513976932952	0.0
9	0.0	0.0	0.0	10.354180183290378	0.0
10	0.0	0.0	0.0	11.853954988899007	0.0
11	0.0	0.0	0.0	13.868403143594659	0.0
12	0.0	0.0	0.0	15.367979540047896	0.0
13	0.0	0.0	0.0	16.078482725506884	0.0
14	0.0	0.0	0.0	16.414587834741045	0.0
15	0.0	0.0	0.0	16.83164387937517	0.0
16	0.0	0.0	0.0	17.650081645367443	0.0
17	0.0	0.0	0.0	18.669111067461095	0.0
18	0.0	0.0	0.0	19.720282772728265	0.0
19	0.0	0.0	0.0	20.440706415956857	0.0
20	0.0	0.0	0.0	21.14565414506487	0.0
21	0.0	0.0	0.0	21.96448872936793	0.0
22	0.0	0.0	0.0	22.84423492437635	0.0
23	0.0	0.0	0.0	23.767234315260243	0.0
24	0.0	0.0	0.0	24.506110009940297	0.0
25	0.0	0.0	0.0	25.139828852262557	0.0
26	0.0	0.0	0.0	25.711644038102495	0.0
27	0.0	0.0	0.0	26.295760591576737	0.0
28	0.0	0.0	0.0	26.90785283596126	0.0
29	0.0	0.0	0.0	27.55109531774234	0.0
30	0.0	0.0	0.0	28.282034646206714	0.0
31	1.984091553920664E-4	0.0	0.0	28.939364178020632	0.0
32	1.984091553920664E-4	0.0	0.0	29.57129733794436	0.0
33	1.984091553920664E-4	0.0	0.0	30.149858435067628	0.0
34	1.984091553920664E-4	0.0	0.0	30.716118164556587	0.0
35	1.984091553920664E-4	0.0	0.0	31.316504268772977	0.0
36	1.984091553920664E-4	0.0	0.0	31.878200587687918	0.0
37	1.984091553920664E-4	0.0	0.0	32.47938032852588	0.0
38	1.984091553920664E-4	0.0	0.0	33.061909608756984	0.0
39	3.968183107841328E-4	0.0	0.0	33.660113212264065	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	45	3.8380676E-10	45.000004	1
TCGTTAG	20	7.029514E-4	45.0	1
TAAACGG	25	3.8876773E-5	45.0	2
GTCGTAG	20	7.029514E-4	45.0	1
CGAATAT	55	1.8189894E-12	45.0	14
TTGCCGT	20	7.029514E-4	45.0	26
TTCGCAA	20	7.029514E-4	45.0	14
AACACGG	20	7.029514E-4	45.0	2
CGTTTTT	3320	0.0	43.64458	1
CGACAGG	75	0.0	42.0	2
GCTACGA	60	3.6379788E-12	41.249996	10
GCGACAG	50	1.0786607E-9	40.5	1
TATACGG	50	1.0786607E-9	40.5	2
CGTTTTC	140	0.0	40.17857	1
CGCGAGG	85	0.0	39.705883	2
TTTACGG	40	3.4530785E-7	39.375	2
CGTTCTG	80	0.0	39.375	1
ACGGGTC	40	3.4530785E-7	39.375	5
TTGGTCG	40	3.4530785E-7	39.375	1
CGTAAGG	75	0.0	39.0	2
>>END_MODULE