FastQCFastQC Report
Sat 18 Jun 2016
SRR3550703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316480
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT20230.6392189079878665No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC14560.46006066734074824No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC10090.318819514661274No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCC7700.24330131445904954No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.18768958543983824No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC5140.16241152679474216No Hit
ACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC4560.14408493427704752No Hit
AGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG3470.10964357937310414No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3340.10553589484327605No Hit
GCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG3230.10206016177957533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT253.884377E-545.00000428
AAGACGG253.884377E-545.0000042
GCGATGA453.8380676E-1045.0000049
CGGTCTA253.884377E-545.00000431
ATTGCGG351.208191E-745.0000042
ACGGTCT253.884377E-545.00000430
CGTGAAG207.025531E-445.034
TTATGCG207.025531E-445.01
GTAATTG207.025531E-445.01
GTATGCG207.025531E-445.01
GTTCCGA207.025531E-445.09
ACGTAAT207.025531E-445.026
AATACGG207.025531E-445.02
TCGGTCT406.7884685E-945.013
TAGACGG302.1604992E-644.9999962
CTAACGG302.1604992E-644.9999962
CGGTAGT900.042.50000412
TAACGGG850.042.352943
TAGGGAT1050.040.7142835
ATAGCGG501.0768417E-940.5000042