FastQCFastQC Report
Sat 18 Jun 2016
SRR3550702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814288
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44480.5462440807183699No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT34850.4279812547894602Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC22020.2704202935570707RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC21840.26820977344624997No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC18030.22142043110054427Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT8590.10549093195527871No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT8560.1051225119368086No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG8500.10438567189986835Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG351.2113378E-745.0000041
CTCGACG207.032075E-445.01
GCGTAGC207.032075E-445.037
CGTAAGG1700.043.6764682
CGTTTTT27300.041.620881
TCGTAAG650.041.5384641
ACGGGTC556.002665E-1140.9090925
ACGTAAG501.0804797E-940.51
GCTAGCG900.040.01
TATAGCG750.039.01
GCGTAAG750.039.01
AGGGCCG1250.037.86
TGCGACA301.13962924E-437.50000430
GACACGA850.037.05882325
GCGATTA800.036.56259
TACGAAT1300.036.34615712
CTAGCGG1550.036.290322
TGATCGG250.002106748836.02
CTATGCG250.002106748836.01
ACACGCG4000.036.036
Produced by FastQC (version 0.11.5)