##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550702_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814288
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.283396046607585	33.0	31.0	34.0	30.0	34.0
2	32.43179931424754	34.0	31.0	34.0	30.0	34.0
3	32.39175328630657	34.0	31.0	34.0	30.0	34.0
4	35.91250761401371	37.0	35.0	37.0	35.0	37.0
5	36.00874751930521	37.0	35.0	37.0	35.0	37.0
6	36.05305616685988	37.0	35.0	37.0	35.0	37.0
7	36.0872885269094	37.0	36.0	37.0	35.0	37.0
8	36.00371858605309	37.0	35.0	37.0	35.0	37.0
9	37.84707376259996	39.0	38.0	39.0	35.0	39.0
10	37.60407865521781	39.0	37.0	39.0	35.0	39.0
11	37.55165494272297	39.0	37.0	39.0	35.0	39.0
12	37.53812287544456	39.0	37.0	39.0	35.0	39.0
13	37.542493564930346	39.0	37.0	39.0	35.0	39.0
14	38.83038187962981	40.0	38.0	41.0	35.0	41.0
15	38.903871848780774	40.0	38.0	41.0	35.0	41.0
16	38.931518086966776	40.0	38.0	41.0	35.0	41.0
17	38.89343205352406	40.0	38.0	41.0	35.0	41.0
18	38.82056225807086	40.0	38.0	41.0	35.0	41.0
19	38.754211040811114	40.0	38.0	41.0	35.0	41.0
20	38.669898119584225	40.0	38.0	41.0	35.0	41.0
21	38.59179553180201	40.0	38.0	41.0	35.0	41.0
22	38.56132965240799	40.0	38.0	41.0	35.0	41.0
23	38.51746310887548	40.0	38.0	41.0	35.0	41.0
24	38.494442998054744	40.0	38.0	41.0	35.0	41.0
25	38.407105348476215	40.0	37.0	41.0	34.0	41.0
26	38.42739423889336	40.0	38.0	41.0	34.0	41.0
27	38.417826371013696	40.0	38.0	41.0	34.0	41.0
28	38.34477727781817	40.0	37.0	41.0	34.0	41.0
29	38.31449560843338	40.0	37.0	41.0	34.0	41.0
30	38.22966935531409	40.0	37.0	41.0	34.0	41.0
31	38.14258714361504	40.0	37.0	41.0	34.0	41.0
32	38.03753463148173	40.0	37.0	41.0	34.0	41.0
33	37.93189387538561	40.0	37.0	41.0	34.0	41.0
34	37.811501581749944	40.0	37.0	41.0	33.0	41.0
35	37.72402638869786	40.0	37.0	41.0	33.0	41.0
36	37.618397913269014	40.0	37.0	41.0	33.0	41.0
37	37.55005845597626	40.0	37.0	41.0	33.0	41.0
38	37.49607141453638	40.0	36.0	41.0	33.0	41.0
39	37.400031684121586	40.0	36.0	41.0	33.0	41.0
40	37.31054983003556	40.0	36.0	41.0	33.0	41.0
41	37.24089020100996	40.0	36.0	41.0	32.0	41.0
42	37.18006282789381	40.0	36.0	41.0	32.0	41.0
43	37.11919001630873	40.0	35.0	41.0	32.0	41.0
44	37.03172587585719	40.0	35.0	41.0	32.0	41.0
45	36.90900025543788	39.0	35.0	41.0	31.0	41.0
46	36.81807419487945	39.0	35.0	41.0	31.0	41.0
47	36.731564262275754	39.0	35.0	41.0	31.0	41.0
48	36.69050630735072	39.0	35.0	41.0	31.0	41.0
49	36.62311860177235	39.0	35.0	41.0	31.0	41.0
50	36.52472098323935	39.0	35.0	41.0	31.0	41.0
51	35.55151125891577	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	14.0
10	18.0
11	12.0
12	7.0
13	14.0
14	14.0
15	17.0
16	37.0
17	65.0
18	129.0
19	263.0
20	446.0
21	670.0
22	1052.0
23	1686.0
24	2753.0
25	4236.0
26	5959.0
27	7313.0
28	7820.0
29	8646.0
30	9951.0
31	11963.0
32	15401.0
33	20472.0
34	37104.0
35	51397.0
36	52464.0
37	79886.0
38	152510.0
39	341485.0
40	474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.23528776059576	20.876643153282377	24.748123514039257	13.139945572082604
2	33.664870414398834	24.708334152044486	23.850652349046037	17.77614308451064
3	31.306368262825927	23.764319258051206	28.32695557345804	16.602356905664823
4	28.40813078419429	25.516279252549467	27.269221700430315	18.806368262825927
5	25.108806712121513	29.711969229560058	25.62017369775804	19.55905036056039
6	25.610103550586526	33.78915076729609	25.262069439805078	15.338676242312301
7	77.61713300453893	6.3759996463167825	10.551303715638694	5.455563633505591
8	77.59097518322756	5.9186675967225355	10.666496374747018	5.823860845302891
9	70.41329357671978	8.808062994910891	13.621102116204586	7.157541312164738
10	35.352602519010475	30.781124123160357	19.5317872791936	14.33448607863557
11	26.170224785333936	25.27840272729059	28.936936317371742	19.61443617000373
12	24.6353869883874	23.44858330222231	31.43556088263612	20.48046882675417
13	23.18184720884994	24.207896960289236	32.78373253689112	19.8265232939697
14	19.675716699742594	27.598834810288253	30.98879020690468	21.736658283064468
15	19.428998094040438	26.563328945041558	33.56809875621401	20.439574204703987
16	21.375238244945276	26.93346825693121	31.499911579195565	20.19138191892795
17	21.154554653881675	26.894906961664667	29.175181262649087	22.77535712180457
18	21.579711355196196	26.571311378775075	30.359774428703357	21.48920283732537
19	22.153832550645472	28.0540791464445	28.615305641247325	21.176782661662706
20	23.315829288900243	27.71979938301928	28.954006444894194	20.010364883186295
21	22.794514962765017	27.885465584658007	29.166707602224275	20.1533118503527
22	21.65929007918574	25.813594207454855	28.14176311084039	24.38535260251901
23	21.12029159216395	27.322151376417185	29.07656750437192	22.48098952704694
24	21.382483838641857	25.99534808323345	29.96531939559468	22.656848682530015
25	21.502220344644645	28.16902619220718	27.50722103236202	22.82153243078616
26	21.532430786159196	27.900816418760932	28.08490362132317	22.481849173756704
27	22.78923427583361	26.211733440748237	28.365639675397404	22.63339260802075
28	20.52750378244552	26.798135303479846	28.777287642701353	23.897073271373273
29	21.98558740887745	24.790860236181793	27.81350087438351	25.41005148055725
30	22.57088401155365	25.231244964926415	27.742887037510073	24.454983986009864
31	21.979938301927575	26.63504804197041	26.96662605859352	24.4183875975085
32	21.96200852769536	26.657767276442733	26.75834594148508	24.62187825437683
33	20.435030357809524	26.56013597154815	26.65383746291238	26.350996207729942
34	20.00803155640265	26.607662153930793	28.284464464661152	25.099841825005402
35	21.17911598844635	25.56884050851787	27.706904682372823	25.54513882066296
36	20.15097852356906	28.290481991629495	26.978783919203035	24.57975556559841
37	19.828365394062054	28.67891949776983	28.59774428703358	22.894970821134535
38	19.123823512074352	27.305695282258856	28.862515473640777	24.707965732026015
39	20.76869608787063	26.774924842316235	27.53694024718527	24.919438822627864
40	21.233519344507105	26.172558112117578	29.755442791739533	22.838479751635784
41	19.707400821331028	27.417694967873775	28.52897255025249	24.345931660542707
42	21.69748295443381	26.996836500108067	28.634954708899063	22.670725836559054
43	21.873710529935355	24.925210736250563	29.430250722103235	23.770828011710847
44	21.42301004067357	25.366946338396247	27.579185742636515	25.63085787829367
45	21.56460613443892	23.889827677676696	27.958044328296623	26.587521859587763
46	21.98423653547639	25.17942054899495	27.986289863046	24.85005305248266
47	20.17578547147938	25.206315210343273	30.171511799265126	24.44638751891223
48	19.83045310750005	24.553229324268564	30.02979289882695	25.586524669404437
49	21.03788831469947	24.22742322126815	30.130985597233412	24.603702866798972
50	19.293542333916257	23.543758473660425	30.451142593283947	26.711556599139367
51	19.70383942781915	22.91142691529287	28.818305071424362	28.566428585463623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	362.0
1	767.0
2	1172.0
3	4213.0
4	7254.0
5	4927.5
6	2601.0
7	2541.0
8	2481.0
9	2386.0
10	2291.0
11	2409.0
12	2527.0
13	2546.5
14	2566.0
15	2470.5
16	2375.0
17	2387.0
18	2399.0
19	2406.0
20	2413.0
21	2675.0
22	2937.0
23	3289.5
24	3642.0
25	4593.0
26	6428.0
27	7312.0
28	8699.0
29	10086.0
30	11542.0
31	12998.0
32	14827.0
33	16656.0
34	18551.0
35	20446.0
36	22233.0
37	24020.0
38	26462.5
39	28905.0
40	32764.0
41	36623.0
42	39489.0
43	42355.0
44	48007.0
45	53659.0
46	64263.5
47	74868.0
48	83699.5
49	92531.0
50	88456.0
51	84381.0
52	73175.0
53	61969.0
54	54499.5
55	47030.0
56	42083.0
57	37136.0
58	34221.5
59	31307.0
60	29124.0
61	26941.0
62	24328.5
63	21716.0
64	18524.0
65	15332.0
66	12806.5
67	10281.0
68	8345.5
69	6410.0
70	5180.0
71	3950.0
72	3432.0
73	2914.0
74	2419.5
75	1518.0
76	1111.0
77	789.5
78	468.0
79	345.5
80	223.0
81	169.0
82	115.0
83	75.0
84	35.0
85	22.5
86	10.0
87	8.0
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	814288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.61912749933471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.32869845357308	26.48613830532326
2	9.982013930126005	7.3106528161483855
3	3.6897570227569663	4.05347048573709
4	2.0169612210372256	2.9543744045750397
5	1.4969314260883821	2.7408161374845705
6	1.2023483963725408	2.6417369535232016
7	1.0572668135371144	2.7101331772011648
8	0.9317930146092551	2.7297157763972613
9	0.8120435633269408	2.676269410243499
>10	6.359265831245475	37.79388175764248
>50	0.07240513902445786	1.8554956188849638
>100	0.04209599025216339	2.656344565906606
>500	0.006735358440338861	1.64942472465077
>1k	0.0016838396100847153	1.7415458662817325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4448	0.5462440807183699	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	3485	0.4279812547894602	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	2202	0.2704202935570707	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	2184	0.26820977344624997	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1803	0.22142043110054427	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT	859	0.10549093195527871	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	856	0.1051225119368086	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	850	0.10438567189986835	Illumina PCR Primer Index 7 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4561334564674906E-4	0.0	0.0	0.4394022753620341	0.0
2	2.4561334564674906E-4	0.0	0.0	1.1201196628219992	0.0
3	2.4561334564674906E-4	0.0	0.0	1.730591633426994	0.0
4	2.4561334564674906E-4	0.0	0.0	2.4915017782406226	0.0
5	2.4561334564674906E-4	0.0	0.0	4.166953215569921	0.0
6	2.4561334564674906E-4	0.0	0.0	5.491300375297192	0.0
7	2.4561334564674906E-4	0.0	0.0	6.689525082034858	0.0
8	2.4561334564674906E-4	0.0	0.0	8.65185290707956	0.0
9	2.4561334564674906E-4	0.0	0.0	9.52783290432869	0.0
10	2.4561334564674906E-4	0.0	0.0	10.899706246438607	0.0
11	2.4561334564674906E-4	0.0	0.0	12.716999390878902	0.0
12	2.4561334564674906E-4	0.0	0.0	14.091328866445288	0.0
13	2.4561334564674906E-4	0.0	0.0	14.722923584775902	0.0
14	2.4561334564674906E-4	0.0	0.0	15.012378912620596	0.0
15	2.4561334564674906E-4	0.0	0.0	15.43974613404594	0.0
16	2.4561334564674906E-4	0.0	0.0	16.178059851060066	0.0
17	2.4561334564674906E-4	0.0	0.0	17.10942565775254	0.0
18	2.4561334564674906E-4	0.0	0.0	18.088931680191774	0.0
19	2.4561334564674906E-4	0.0	0.0	18.73980704615566	0.0
20	3.684200184701236E-4	0.0	0.0	19.382208751694733	0.0
21	3.684200184701236E-4	0.0	0.0	20.12666280235003	0.0
22	3.684200184701236E-4	0.0	0.0	20.962239404240268	0.0
23	3.684200184701236E-4	0.0	0.0	21.753728410586916	0.0
24	4.912266912934981E-4	0.0	0.0	22.411480950228913	0.0
25	4.912266912934981E-4	0.0	0.0	22.996163519540996	0.0
26	6.140333641168727E-4	0.0	0.0	23.506548051794944	0.0
27	6.140333641168727E-4	0.0	0.0	24.0422307586505	0.0
28	7.368400369402472E-4	0.0	0.0	24.58036959896253	0.0
29	7.368400369402472E-4	0.0	0.0	25.175245122118955	0.0
30	7.368400369402472E-4	0.0	0.0	25.867383530151493	0.0
31	7.368400369402472E-4	0.0	0.0	26.436960878706305	0.0
32	7.368400369402472E-4	0.0	0.0	27.01132768750123	0.0
33	7.368400369402472E-4	0.0	0.0	27.56997524217476	0.0
34	7.368400369402472E-4	0.0	0.0	28.112412316035606	0.0
35	7.368400369402472E-4	0.0	0.0	28.67818265773289	0.0
36	8.596467097636217E-4	0.0	0.0	29.235970565696658	0.0
37	8.596467097636217E-4	0.0	0.0	29.773126952626097	0.0
38	8.596467097636217E-4	0.0	0.0	30.319248226671643	0.0
39	9.824533825869963E-4	0.0	0.0	30.865001080698722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAAGCG	35	1.2113378E-7	45.000004	1
CTCGACG	20	7.032075E-4	45.0	1
GCGTAGC	20	7.032075E-4	45.0	37
CGTAAGG	170	0.0	43.676468	2
CGTTTTT	2730	0.0	41.62088	1
TCGTAAG	65	0.0	41.538464	1
ACGGGTC	55	6.002665E-11	40.909092	5
ACGTAAG	50	1.0804797E-9	40.5	1
GCTAGCG	90	0.0	40.0	1
TATAGCG	75	0.0	39.0	1
GCGTAAG	75	0.0	39.0	1
AGGGCCG	125	0.0	37.8	6
TGCGACA	30	1.13962924E-4	37.500004	30
GACACGA	85	0.0	37.058823	25
GCGATTA	80	0.0	36.5625	9
TACGAAT	130	0.0	36.346157	12
CTAGCGG	155	0.0	36.29032	2
TGATCGG	25	0.0021067488	36.0	2
CTATGCG	25	0.0021067488	36.0	1
ACACGCG	400	0.0	36.0	36
>>END_MODULE