##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550700_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	527010
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23807328134191	33.0	31.0	34.0	30.0	34.0
2	32.41796360600368	34.0	31.0	34.0	30.0	34.0
3	32.29027342934669	34.0	31.0	34.0	30.0	34.0
4	35.89106658317679	37.0	35.0	37.0	35.0	37.0
5	36.004897440276274	37.0	35.0	37.0	35.0	37.0
6	36.06269330752737	37.0	35.0	37.0	35.0	37.0
7	36.150171723496705	37.0	36.0	37.0	35.0	37.0
8	36.10120111572835	37.0	37.0	37.0	35.0	37.0
9	37.9431509838523	39.0	38.0	39.0	35.0	39.0
10	37.65028936832318	39.0	37.0	39.0	35.0	39.0
11	37.608366065160055	39.0	37.0	39.0	35.0	39.0
12	37.61361454241855	39.0	37.0	39.0	35.0	39.0
13	37.62664655319633	39.0	37.0	39.0	35.0	39.0
14	38.95528168345952	40.0	38.0	41.0	35.0	41.0
15	39.00575510901121	40.0	38.0	41.0	35.0	41.0
16	39.02343788542912	40.0	38.0	41.0	35.0	41.0
17	38.988493576971976	40.0	38.0	41.0	35.0	41.0
18	38.80563936168194	40.0	38.0	41.0	35.0	41.0
19	38.601030340980245	40.0	37.0	41.0	35.0	41.0
20	38.433061991233565	40.0	37.0	41.0	35.0	41.0
21	38.335386425305025	40.0	37.0	41.0	35.0	41.0
22	38.32750801692568	40.0	36.0	41.0	35.0	41.0
23	38.27327375192122	40.0	36.0	41.0	35.0	41.0
24	38.24183791578908	40.0	36.0	41.0	34.0	41.0
25	38.17598337792452	40.0	36.0	41.0	34.0	41.0
26	38.17608015028178	40.0	36.0	41.0	34.0	41.0
27	38.151200166979756	40.0	36.0	41.0	34.0	41.0
28	38.06680328646515	40.0	36.0	41.0	34.0	41.0
29	38.0031821028064	40.0	36.0	41.0	34.0	41.0
30	37.84259501717235	40.0	36.0	41.0	34.0	41.0
31	37.64773723458758	40.0	36.0	41.0	33.0	41.0
32	37.35482248913683	40.0	35.0	41.0	33.0	41.0
33	37.00002846245802	40.0	35.0	41.0	31.0	41.0
34	36.65249995256257	40.0	35.0	41.0	30.0	41.0
35	36.437661524449254	40.0	35.0	41.0	30.0	41.0
36	36.28505531204341	40.0	35.0	41.0	29.0	41.0
37	36.17731731845696	40.0	35.0	41.0	28.0	41.0
38	36.0961746456424	40.0	35.0	41.0	27.0	41.0
39	35.98144057987515	40.0	35.0	41.0	26.0	41.0
40	35.88011612682871	39.0	35.0	41.0	26.0	41.0
41	35.77968539496404	39.0	35.0	41.0	25.0	41.0
42	35.68708942904309	39.0	35.0	41.0	24.0	41.0
43	35.603161230337186	39.0	35.0	41.0	24.0	41.0
44	35.521570748183144	39.0	35.0	41.0	23.0	41.0
45	35.385852260867914	39.0	35.0	41.0	23.0	41.0
46	35.276620936984116	39.0	35.0	41.0	23.0	41.0
47	35.18593005825316	39.0	35.0	41.0	23.0	41.0
48	35.099087303846225	39.0	35.0	41.0	23.0	41.0
49	35.01552721959735	39.0	35.0	41.0	23.0	41.0
50	34.886026830610426	38.0	35.0	40.0	23.0	41.0
51	33.91613631619894	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	7.0
11	5.0
12	9.0
13	11.0
14	9.0
15	15.0
16	27.0
17	60.0
18	119.0
19	198.0
20	373.0
21	662.0
22	995.0
23	1747.0
24	3166.0
25	6237.0
26	9338.0
27	10176.0
28	9105.0
29	8274.0
30	8340.0
31	8820.0
32	10600.0
33	13618.0
34	22469.0
35	32723.0
36	33702.0
37	49884.0
38	97567.0
39	198527.0
40	221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.030397905163085	20.037760194303715	23.156107094742033	18.77573480579116
2	38.85561943796133	21.65442780971898	23.564068992998237	15.925883759321454
3	28.273467296635737	22.292745868199844	34.03578679721447	15.398000037949943
4	25.071440769624864	23.99157511242671	33.671277584865564	17.265706533082863
5	22.675471053680198	27.510293922316464	31.936775393256294	17.877459630747044
6	23.058006489440427	31.254435399707788	31.2783438644428	14.409214246408986
7	73.50581582892165	4.907307261721789	16.44162349860534	5.145253410751219
8	74.4875808808182	3.9896776152255176	16.406899299823532	5.11584220413275
9	68.15790971708317	6.800819718790915	18.647653744710727	6.393616819415191
10	33.0369442705072	27.678412174342043	25.258533993662358	14.026109561488397
11	24.60977970057494	24.22724426481471	32.544164247357735	18.618811787252614
12	22.379461490294304	21.680044021935068	36.088499269463576	19.851995218307053
13	20.957477087721298	22.6428341018197	37.677653175461565	18.72203563499744
14	18.39263012087057	26.52606212405837	35.34657786379765	19.73472989127341
15	17.583157814842224	25.649038917667593	38.23039411016869	18.537409157321495
16	19.788049562626895	26.342953644143375	36.21525208250318	17.653744710726553
17	20.068499648963016	25.598944991556138	33.42365420011005	20.908901159370792
18	20.674180755583386	25.45112995958331	34.2716456993226	19.60304358551071
19	20.116885827593403	27.911045331208133	32.40868294719265	19.563385894005805
20	22.28895087379746	26.417145784709966	32.047778979526	19.246124361966565
21	20.44401434507884	27.158497941215536	33.51833931044952	18.879148403256103
22	20.45767632492742	24.433122711144	32.72518548035141	22.384015483577162
23	19.291284795354926	27.183355154551148	32.96483937686192	20.56052067323201
24	19.922582114191382	24.727993776209182	34.11016868750119	21.239255422098253
25	20.550274188345572	26.603290260146867	31.65290981195803	21.193525739549536
26	20.463179066810877	27.430978539306654	32.03468624883779	20.071156145044686
27	21.65670480636041	26.1649684066716	31.68364926661733	20.494677520350656
28	19.16851672643783	27.408208572892356	31.66543329348589	21.757841407183925
29	21.437733629342898	25.335382630310622	31.15140130168308	22.075482438663403
30	22.000341549496213	26.159655414508265	30.272860097531357	21.567142938464166
31	20.69619172311721	28.981044002960093	28.23039411016869	22.09237016375401
32	21.345325515644863	28.81045900457297	27.98884271645699	21.855372763325175
33	19.912525379025066	29.162254985673897	27.408398322612477	23.516821312688563
34	19.734160642113054	29.571165632530693	27.86873114362156	22.825942581734694
35	20.82958577636098	28.26606705755109	27.211248363408664	23.693098802679266
36	20.49885201419328	31.685357014098404	25.781484222310773	22.034306749397544
37	20.48936452818732	31.525018500597714	26.932506024553614	21.053110946661356
38	20.504734255516972	29.27079182558206	26.99910817631544	23.22536574258553
39	21.236788675736705	27.87442363522514	27.28904574865752	23.59974194038064
40	22.75326843892905	26.236504051156523	29.532836189066618	21.4773913208478
41	21.51818751067342	28.360182918730192	27.94861577579173	22.173013794804653
42	22.586857934384547	28.509515948463974	28.002694446025693	20.900931671125786
43	21.56676343902393	27.695299899432648	29.16434223259521	21.57359442894822
44	21.729948198326408	27.180888408189595	27.3294624390429	23.759700954441094
45	21.894081706229485	24.54412629741371	28.018443672795584	25.543348323561222
46	21.90603593859699	26.275023244340716	27.217699853892714	24.60124096316958
47	21.202823475835373	26.813912449479137	28.985787745963076	22.997476328722417
48	20.730915921899015	25.836132141705093	29.08673459706647	24.346217339329424
49	21.614580368493957	24.837669114438057	29.47932676799302	24.06842374907497
50	19.23151363351739	24.62590842678507	30.713079448207814	25.429498491489728
51	19.68084097075957	24.275820193165217	28.899641373028974	27.14369746304624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	210.0
1	337.0
2	464.0
3	6370.0
4	12276.0
5	8194.5
6	4113.0
7	4100.5
8	4088.0
9	4293.5
10	4499.0
11	4406.0
12	4313.0
13	4270.0
14	4227.0
15	4109.5
16	3992.0
17	3801.0
18	3610.0
19	3364.0
20	3118.0
21	3113.5
22	3109.0
23	3014.0
24	2919.0
25	3143.5
26	3746.5
27	4125.0
28	4571.5
29	5018.0
30	5970.5
31	6923.0
32	7943.0
33	8963.0
34	10401.5
35	11840.0
36	12993.5
37	14147.0
38	15496.5
39	16846.0
40	19289.5
41	21733.0
42	24351.0
43	26969.0
44	30694.5
45	34420.0
46	42640.5
47	50861.0
48	56063.0
49	61265.0
50	59216.0
51	57167.0
52	48980.5
53	40794.0
54	35122.0
55	29450.0
56	25343.0
57	21236.0
58	19280.0
59	17324.0
60	15981.0
61	14638.0
62	13143.0
63	11648.0
64	9393.0
65	7138.0
66	5547.5
67	3957.0
68	3217.0
69	2477.0
70	1931.5
71	1386.0
72	1080.5
73	775.0
74	762.5
75	633.0
76	516.0
77	364.0
78	212.0
79	149.0
80	86.0
81	54.5
82	23.0
83	15.5
84	8.0
85	7.5
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	527010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.263913588167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43452571742411	35.653309050726946
2	9.541764675742195	9.01962282225807
3	3.989301679298359	5.656500295424614
4	2.4660477411066584	4.662202693598381
5	1.7310387957881268	4.090783403094734
6	1.2927823171419908	3.666117103542562
7	1.0673204303872614	3.5312018414896116
8	0.8137810508935667	3.076998181529701
9	0.6496933771102447	2.76363464710887
>10	2.9417763597631	20.450336128685336
>50	0.04824660695033796	1.5534703580461193
>100	0.01849453264617422	1.4234919196482085
>500	0.001608220230102106	0.5505080885356908
>1k	0.003216440460204212	2.3918692821535177
>5k	4.020550575255265E-4	1.509954184157611
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7946	1.5077512760668679	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTT	2419	0.45900457296825486	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC	1997	0.37893019107796816	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTG	1553	0.29468131534505987	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCT	1551	0.2943018159048216	No Hit
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT	1549	0.2939223164645832	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	1363	0.25862886852241895	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCC	1147	0.21764292897667975	No Hit
CGTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCT	1008	0.19126771788011615	No Hit
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	984	0.18671372459725621	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTC	806	0.15293827441604524	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG	557	0.10569059410637369	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGT	550	0.10436234606553955	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7949944023832566E-4	0.0	0.0	0.3077740460332821	0.0
2	3.7949944023832566E-4	0.0	0.0	0.9081421604903133	0.0
3	3.7949944023832566E-4	0.0	0.0	1.6174266142957439	0.0
4	3.7949944023832566E-4	0.0	0.0	2.39350296958312	0.0
5	3.7949944023832566E-4	0.0	0.0	4.257983719474014	0.0
6	3.7949944023832566E-4	0.0	0.0	6.115443729720499	0.0
7	3.7949944023832566E-4	0.0	0.0	7.605738031536403	0.0
8	3.7949944023832566E-4	0.0	0.0	9.711580425418873	0.0
9	3.7949944023832566E-4	0.0	0.0	10.737936661543424	0.0
10	3.7949944023832566E-4	0.0	0.0	12.24398019012922	0.0
11	3.7949944023832566E-4	0.0	0.0	14.047361530141742	0.0
12	3.7949944023832566E-4	0.0	0.0	15.408246522836379	0.0
13	3.7949944023832566E-4	0.0	0.0	16.116392478321096	0.0
14	3.7949944023832566E-4	0.0	0.0	16.45528547845392	0.0
15	3.7949944023832566E-4	0.0	0.0	16.889053338646324	0.0
16	3.7949944023832566E-4	0.0	0.0	17.57461907743686	0.0
17	3.7949944023832566E-4	0.0	0.0	18.48333048708753	0.0
18	3.7949944023832566E-4	0.0	0.0	19.41784785867441	0.0
19	3.7949944023832566E-4	0.0	0.0	20.070017646723972	0.0
20	7.589988804766513E-4	0.0	0.0	20.674560255023625	0.0
21	7.589988804766513E-4	0.0	0.0	21.41705090984991	0.0
22	7.589988804766513E-4	0.0	0.0	22.23031821028064	0.0
23	7.589988804766513E-4	0.0	0.0	23.051934498396616	0.0
24	7.589988804766513E-4	0.0018974972011916282	0.0	23.648507618451262	0.0
25	7.589988804766513E-4	0.0018974972011916282	0.0	24.21111553860458	0.0
26	7.589988804766513E-4	0.0018974972011916282	0.0	24.724957780687273	0.0
27	7.589988804766513E-4	0.0018974972011916282	0.0	25.262518737784863	0.0
28	7.589988804766513E-4	0.0018974972011916282	0.0	25.82892165234056	0.0
29	7.589988804766513E-4	0.0018974972011916282	0.0	26.412971290867347	0.0
30	7.589988804766513E-4	0.0018974972011916282	0.0	27.0898085425324	0.0
31	7.589988804766513E-4	0.0018974972011916282	0.0	27.70630538319956	0.0
32	7.589988804766513E-4	0.0018974972011916282	0.0	28.29794501053111	0.0
33	7.589988804766513E-4	0.0018974972011916282	0.0	28.874404660253127	0.0
34	7.589988804766513E-4	0.0018974972011916282	0.0	29.407411624067855	0.0
35	7.589988804766513E-4	0.0018974972011916282	0.0	29.943834082844727	0.0
36	7.589988804766513E-4	0.0018974972011916282	0.0	30.508908749359595	0.0
37	7.589988804766513E-4	0.0018974972011916282	0.0	31.054439194702187	0.0
38	7.589988804766513E-4	0.0018974972011916282	0.0	31.602436386406332	0.0
39	7.589988804766513E-4	0.0018974972011916282	0.0	32.17130604732358	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGA	20	7.029805E-4	45.000004	21
CCGATAC	20	7.029805E-4	45.000004	11
GCGTTAG	20	7.029805E-4	45.000004	1
TGCGATG	20	7.029805E-4	45.000004	1
AATAGCG	20	7.029805E-4	45.000004	1
GCGTCAT	20	7.029805E-4	45.000004	37
CGTCATA	20	7.029805E-4	45.000004	38
TGTTACG	25	3.887921E-5	45.0	1
GCTACGA	25	3.887921E-5	45.0	10
TTAGGCG	30	2.1632568E-6	44.999996	1
TGTAGCG	30	2.1632568E-6	44.999996	1
CGTTTTT	5220	0.0	44.008617	1
ATTGCGG	95	0.0	42.63158	2
CGTTTTC	135	0.0	41.666664	1
CGTTTCT	330	0.0	41.590908	1
CTTGCGG	60	3.6379788E-12	41.249996	2
CGTTAGG	50	1.0786607E-9	40.5	2
AGCGATG	45	1.9248546E-8	40.0	1
CGTTTGA	45	1.9248546E-8	40.0	11
GTTTTTT	5890	0.0	38.73514	2
>>END_MODULE