SRR3550697_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550697_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	363342
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1988	0.5471429121874157	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1813	0.49897892343852346	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	1676	0.46127340081796214	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	777	0.2138481100450815	No Hit
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	699	0.1923807322027181	TruSeq Adapter, Index 21 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	556	0.1530238728250519	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC	538	0.14806986255373725	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	457	0.12577681633282142	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	436	0.11999713768295435	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	415	0.11421745903308729	TruSeq Adapter, Index 21 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	25	3.88552E-5	45.000004	1
ACGATTA	25	3.88552E-5	45.000004	8
TGCGAAT	25	3.88552E-5	45.000004	27
ATGCGAA	25	3.88552E-5	45.000004	26
ATACGAA	30	2.1613887E-6	45.000004	19
GATAACG	20	7.0269115E-4	45.0	1
AACCACG	20	7.0269115E-4	45.0	10
TACGGGT	40	6.7921064E-9	45.0	4
ACGGGTA	55	1.8189894E-12	45.0	5
TCTAGCG	20	7.0269115E-4	45.0	1
CGTTAGG	35	1.208864E-7	45.0	2
CGGTCTA	35	1.208864E-7	45.0	31
ATAACGG	35	1.208864E-7	45.0	2
CGTCATA	35	1.208864E-7	45.0	38
CGATATG	20	7.0269115E-4	45.0	10
TCGAATG	20	7.0269115E-4	45.0	1
AATACGG	35	1.208864E-7	45.0	2
GCGTAAG	40	6.7921064E-9	45.0	1
CGGGTAT	55	6.002665E-11	40.909092	6
CGAGACA	55	6.002665E-11	40.909092	22