##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550697_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	363342
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31260630480374	33.0	31.0	34.0	30.0	34.0
2	32.448552603332395	34.0	31.0	34.0	30.0	34.0
3	32.42714852673239	34.0	31.0	34.0	30.0	34.0
4	35.93290068310297	37.0	35.0	37.0	35.0	37.0
5	36.0297543361351	37.0	35.0	37.0	35.0	37.0
6	36.07166801525835	37.0	35.0	37.0	35.0	37.0
7	36.09483626996053	37.0	36.0	37.0	35.0	37.0
8	36.01173274765923	37.0	35.0	37.0	35.0	37.0
9	37.8579382510142	39.0	38.0	39.0	35.0	39.0
10	37.659885177050825	39.0	37.0	39.0	35.0	39.0
11	37.57130472117179	39.0	37.0	39.0	35.0	39.0
12	37.34930451200247	39.0	37.0	39.0	35.0	39.0
13	37.251749040848566	39.0	37.0	39.0	34.0	39.0
14	38.403231666033655	40.0	38.0	41.0	34.0	41.0
15	38.488781918963404	40.0	38.0	41.0	35.0	41.0
16	38.55642617699027	40.0	38.0	41.0	35.0	41.0
17	38.5136895817164	40.0	37.0	41.0	35.0	41.0
18	38.479628008873185	40.0	37.0	41.0	34.0	41.0
19	38.439395941014254	40.0	37.0	41.0	34.0	41.0
20	38.39298236922789	40.0	37.0	41.0	34.0	41.0
21	38.31472827253662	40.0	37.0	41.0	34.0	41.0
22	38.30028733259574	40.0	37.0	41.0	34.0	41.0
23	38.28706012517133	40.0	36.0	41.0	34.0	41.0
24	38.24547120894364	40.0	36.0	41.0	34.0	41.0
25	38.18081036599127	40.0	36.0	41.0	34.0	41.0
26	38.187495527629615	40.0	36.0	41.0	34.0	41.0
27	38.17293624188781	40.0	36.0	41.0	34.0	41.0
28	38.09588762102922	40.0	36.0	41.0	34.0	41.0
29	38.0161803479917	40.0	36.0	41.0	34.0	41.0
30	37.92586598851771	40.0	36.0	41.0	34.0	41.0
31	37.81863368396717	40.0	36.0	41.0	34.0	41.0
32	37.62799235981527	40.0	35.0	41.0	33.0	41.0
33	37.57916508413561	40.0	35.0	41.0	33.0	41.0
34	37.437997809226566	40.0	35.0	41.0	33.0	41.0
35	37.30236251245383	40.0	35.0	41.0	33.0	41.0
36	37.27936489588322	39.0	35.0	41.0	33.0	41.0
37	37.24306851396205	39.0	35.0	41.0	33.0	41.0
38	37.1280914400207	39.0	35.0	41.0	33.0	41.0
39	37.090482245377636	39.0	35.0	41.0	33.0	41.0
40	37.002966901706934	39.0	35.0	41.0	32.0	41.0
41	36.917122160388836	39.0	35.0	41.0	32.0	41.0
42	36.88450550720808	39.0	35.0	41.0	32.0	41.0
43	36.87214525158115	39.0	35.0	41.0	32.0	41.0
44	36.81350353110843	39.0	35.0	41.0	32.0	41.0
45	36.69802830391202	39.0	35.0	41.0	32.0	41.0
46	36.60162876848809	39.0	35.0	41.0	31.0	41.0
47	36.48810487089299	39.0	35.0	41.0	31.0	41.0
48	36.453357993295576	38.0	35.0	41.0	31.0	41.0
49	36.422998167016196	38.0	35.0	41.0	31.0	41.0
50	36.31788783019854	38.0	35.0	40.0	31.0	41.0
51	35.261659813619126	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	5.0
12	7.0
13	3.0
14	4.0
15	5.0
16	16.0
17	34.0
18	64.0
19	105.0
20	208.0
21	317.0
22	514.0
23	753.0
24	1248.0
25	1779.0
26	2203.0
27	2725.0
28	3135.0
29	3614.0
30	4406.0
31	5732.0
32	7363.0
33	10873.0
34	23151.0
35	34038.0
36	23867.0
37	35506.0
38	70425.0
39	131086.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77166416213925	22.023603106714887	24.664916249704135	11.539816481441727
2	32.10033522136169	24.945093052826262	24.4318025441595	18.52276918165255
3	31.431543834734217	25.05573261555229	26.444231605484642	17.068491944228853
4	28.5125308937585	26.256529660760386	25.627370356303423	19.60356908917769
5	25.091786801415743	29.400399623495222	25.25059035289067	20.25722322219837
6	26.284051940045465	31.534477159260422	25.631773920989044	16.54969697970507
7	77.40530959811967	6.250860071227658	10.142785584931001	6.201044745721662
8	76.79156277006236	5.73674389418234	10.492043309058682	6.9796500266966115
9	70.34199184239642	8.037606442415136	13.245922574323915	8.37447914086453
10	40.72554232651331	25.295451668125345	19.182753438908797	14.796252566452544
11	32.14189386308217	23.31274666842809	24.750510538280736	19.794848930209007
12	29.534433123613564	21.08784561102212	29.380308359617107	19.997412905747204
13	25.131969329171966	24.68390662241084	30.23377424024748	19.95034980816971
14	19.972642854390628	28.385653186254274	30.13194180689268	21.509762152462418
15	19.949799362584013	24.522350843007416	35.81749426160477	19.710355532803806
16	23.317425455906555	25.552509756648007	30.915225875346092	20.214838912099346
17	21.34160102603057	25.80791650841356	27.451822250111462	25.3986602154444
18	23.936951962613737	25.22554507874124	29.776629181322278	21.06087377732274
19	23.496320271259584	27.82254735208151	26.733490760770845	21.94764161588806
20	25.673883008295217	27.090179500305496	27.425125639204932	19.81081185219435
21	23.694755904905023	28.49051307033043	28.065018632583076	19.749712392181472
22	21.74370152638561	25.213160053062957	28.159420050530905	24.88371837002053
23	21.941586714445343	26.072130389550342	28.217216837029575	23.76906605897474
24	23.951813993427677	23.117888931089716	29.41471120872346	23.515585866759142
25	21.09335006687914	26.106258015863844	26.334142488344312	26.466249428912704
26	19.526781929972312	26.435699698906262	28.8689444105003	25.16857396062112
27	21.94764161588806	25.7030566243374	28.17180507620919	24.177496683565348
28	19.544396188714764	26.928898943694925	28.596749068370848	24.92995579921947
29	22.13616922899087	24.333272784318908	29.854242008906212	23.676315977784014
30	25.214811389820056	23.017707834492022	28.643812165948333	23.123668609739585
31	26.675143528686473	25.091511578622892	25.962041272410012	22.271303620280616
32	25.389853086073177	24.692163306196367	28.69555405100429	21.22242955672617
33	27.09045472309835	25.02215543482449	25.486731509156662	22.400658332920496
34	24.32446565494768	23.984565505776924	30.366982071987277	21.32398676728812
35	21.192430272305433	24.50363569309356	31.066598411414038	23.23733562318697
36	23.951813993427677	28.27281184118544	26.65505226480836	21.120321900578517
37	25.39590798751589	26.252401318867623	27.87594057389457	20.475750119721916
38	23.1963274270522	26.866973815303492	27.19008537411034	22.74661338353397
39	26.15359633623418	24.600789338969896	27.69732098133439	21.548293343461534
40	26.48964336630502	21.436552889564105	29.776353958529427	22.297449785601444
41	20.7242763016662	25.05600783834514	27.73117338485504	26.48854247513362
42	22.58780983205905	23.899246439993174	28.725553335425026	24.78739039252275
43	24.02997726659731	23.082935636397664	28.2937287734421	24.593358323562924
44	23.247794089315303	22.246533568924043	28.103825046375043	26.401847295385615
45	23.846128440972965	21.6559054554662	27.441363783983135	27.0566023195777
46	24.490149776243868	23.13853064055353	28.386203631839972	23.98511595136263
47	20.648039588046522	23.45944041701758	31.64263971685079	24.249880278085108
48	21.0286727105592	21.966631988594767	31.397416208420715	25.607279092425316
49	23.35292919618431	20.32878114833958	32.50518794964524	23.81310170583087
50	21.06637823317976	21.440956454249715	30.939445481116962	26.55321983145356
51	19.78053734498076	20.647764365253675	29.21847735742083	30.353220932344733
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	266.0
1	458.0
2	650.0
3	1183.0
4	1716.0
5	1197.0
6	678.0
7	680.5
8	683.0
9	757.5
10	832.0
11	829.5
12	827.0
13	854.5
14	882.0
15	861.5
16	841.0
17	828.5
18	816.0
19	898.5
20	981.0
21	961.5
22	942.0
23	1120.5
24	1299.0
25	1410.0
26	1720.0
27	1919.0
28	2516.0
29	3113.0
30	3318.0
31	3523.0
32	4458.5
33	5394.0
34	5950.0
35	6506.0
36	7565.0
37	8624.0
38	9432.5
39	10241.0
40	12089.5
41	13938.0
42	15641.5
43	17345.0
44	20485.0
45	23625.0
46	28062.5
47	32500.0
48	37563.0
49	42626.0
50	43051.0
51	43476.0
52	37270.0
53	31064.0
54	27781.0
55	24498.0
56	21925.0
57	19352.0
58	17932.0
59	16512.0
60	15452.5
61	14393.0
62	12956.0
63	11519.0
64	9736.0
65	7953.0
66	6381.5
67	4810.0
68	3858.0
69	2906.0
70	2306.5
71	1707.0
72	1345.5
73	984.0
74	932.0
75	694.5
76	509.0
77	390.5
78	272.0
79	197.0
80	122.0
81	104.5
82	87.0
83	46.0
84	5.0
85	4.5
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	363342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.39592488183315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.04764080444987	26.222405226320543
2	11.067318282255593	8.056105696886267
3	3.9394849954288507	4.301435999002118
4	2.030196703039349	2.9556354679666192
5	1.2823007004730733	2.333525998517001
6	1.0089585482374304	2.203318771831977
7	0.8136938333054872	2.0730597743658166
8	0.6878840618660491	2.00289413144696
9	0.6492415247727659	2.1266771189216764
>10	6.343890790226263	40.57815806898896
>50	0.07944985014182168	2.045085588068082
>100	0.04464324912730933	2.8855920807672186
>500	0.0030266609577836836	0.7077659522400984
>1k	0.0022699957183377624	1.5083401246766612
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1988	0.5471429121874157	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	1813	0.49897892343852346	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	1676	0.46127340081796214	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	777	0.2138481100450815	No Hit
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	699	0.1923807322027181	TruSeq Adapter, Index 21 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	556	0.1530238728250519	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC	538	0.14806986255373725	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	457	0.12577681633282142	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	436	0.11999713768295435	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	415	0.11421745903308729	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4758602088390552	0.0
2	0.0	0.0	0.0	1.9147249698631041	0.0
3	0.0	0.0	0.0	2.7142471830947152	0.0
4	0.0	0.0	0.0	3.618629280402486	0.0
5	0.0	0.0	0.0	5.947839776298914	0.0
6	0.0	0.0	0.0	7.268633959189964	0.0
7	0.0	0.0	0.0	8.55392440180326	0.0
8	0.0	0.0	0.0	10.57543581529248	0.0
9	0.0	0.0	0.0	11.389820059338033	0.0
10	0.0	0.0	0.0	12.847399970275939	0.0
11	0.0	0.0	0.0	15.050283204253844	0.0
12	0.0	0.0	0.0	16.806204622642028	0.0
13	0.0	0.0	0.0	17.499766060626076	0.0
14	0.0	0.0	0.0	17.794254448976446	0.0
15	0.0	0.0	0.0	18.318829092150096	0.0
16	0.0	0.0	0.0	19.269723841449654	0.0
17	0.0	0.0	0.0	20.43694370592995	0.0
18	0.0	0.0	0.0	21.69608798322242	0.0
19	2.752227928508127E-4	0.0	0.0	22.524508589703366	0.0
20	2.752227928508127E-4	0.0	0.0	23.274215477428978	0.0
21	2.752227928508127E-4	0.0	0.0	24.172817896086883	0.0
22	2.752227928508127E-4	0.0	0.0	25.081053112494565	0.0
23	2.752227928508127E-4	0.0	0.0	25.937271221053443	0.0
24	2.752227928508127E-4	0.0	0.0	26.625603425973324	0.0
25	2.752227928508127E-4	0.0	0.0	27.217057207809724	0.0
26	2.752227928508127E-4	0.0	0.0	27.794199404417878	0.0
27	2.752227928508127E-4	0.0	0.0	28.34794766363371	0.0
28	2.752227928508127E-4	0.0	0.0	28.923713746277613	0.0
29	2.752227928508127E-4	0.0	0.0	29.50966307225699	0.0
30	2.752227928508127E-4	0.0	0.0	30.231022012318974	0.0
31	2.752227928508127E-4	0.0	0.0	30.842567058033477	0.0
32	2.752227928508127E-4	0.0	0.0	31.445304974376757	0.0
33	2.752227928508127E-4	0.0	0.0	32.02547462170627	0.0
34	2.752227928508127E-4	0.0	0.0	32.56959008317233	0.0
35	2.752227928508127E-4	0.0	0.0	33.178107678165475	0.0
36	2.752227928508127E-4	0.0	0.0	33.764332226937704	0.0
37	2.752227928508127E-4	0.0	0.0	34.35028155291709	0.0
38	2.752227928508127E-4	0.0	0.0	35.016595934408905	0.0
39	2.752227928508127E-4	0.0	0.0	35.74731244942781	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	25	3.88552E-5	45.000004	1
ACGATTA	25	3.88552E-5	45.000004	8
TGCGAAT	25	3.88552E-5	45.000004	27
ATGCGAA	25	3.88552E-5	45.000004	26
ATACGAA	30	2.1613887E-6	45.000004	19
GATAACG	20	7.0269115E-4	45.0	1
AACCACG	20	7.0269115E-4	45.0	10
TACGGGT	40	6.7921064E-9	45.0	4
ACGGGTA	55	1.8189894E-12	45.0	5
TCTAGCG	20	7.0269115E-4	45.0	1
CGTTAGG	35	1.208864E-7	45.0	2
CGGTCTA	35	1.208864E-7	45.0	31
ATAACGG	35	1.208864E-7	45.0	2
CGTCATA	35	1.208864E-7	45.0	38
CGATATG	20	7.0269115E-4	45.0	10
TCGAATG	20	7.0269115E-4	45.0	1
AATACGG	35	1.208864E-7	45.0	2
GCGTAAG	40	6.7921064E-9	45.0	1
CGGGTAT	55	6.002665E-11	40.909092	6
CGAGACA	55	6.002665E-11	40.909092	22
>>END_MODULE