FastQCFastQC Report
Sat 18 Jun 2016
SRR3550694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630118
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36710.5825892927991265No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT23660.37548522657660943RNA PCR Primer, Index 29 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC18760.2977220139719862Illumina PCR Primer Index 8 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC16540.2624905176490752No Hit
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC16190.2569360024630307RNA PCR Primer, Index 29 (96% over 25bp)
CGTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT12150.1928210271726883RNA PCR Primer, Index 29 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT11460.18187069723448623No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC11240.17837928768897254No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC8050.12775384927902395No Hit
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC7110.11283600849364722Illumina PCR Primer Index 8 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT7040.11172510545643832RNA PCR Primer, Index 29 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTG6700.10632929070428078RNA PCR Primer, Index 29 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT351.2107557E-745.00000414
CACGCTT207.0308585E-445.021
ATTCGGC253.8887927E-545.017
CGCACGG502.1827873E-1145.02
TATCGCG207.0308585E-445.01
ATCGCGG253.8887927E-545.02
CGTTTTT22050.042.2448961
CGTTAGG950.040.263162
CGTATGG451.925946E-840.02
TCGTAAG451.925946E-840.01
GTACGAG403.4549157E-739.3751
CGTAAGG1500.039.0000042
ATCAGCG356.2437957E-638.571431
TGTAACG356.2437957E-638.571431
TACCGGG356.2437957E-638.571433
GCGTTAG659.094947E-1238.0769271
TTGGCCG301.1393365E-437.5000041
TGCGGAT301.1393365E-437.5000044
TGATTCG301.1393365E-437.50000415
TTCGGCA301.1393365E-437.50000418
Produced by FastQC (version 0.11.5)