FastQCFastQC Report
Sat 18 Jun 2016
SRR3550691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638571
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79811.2498218678893966No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTT39970.6259288317195738No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC32380.5070696915456543No Hit
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG28890.4524164110177255TruSeq Adapter, Index 7 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT26630.417024888383594No Hit
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT23970.37536937944253657TruSeq Adapter, Index 7 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC22550.35313222805294947RNA PCR Primer, Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC16290.25510084234955865No Hit
CGTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT15840.24805385775426694TruSeq Adapter, Index 7 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC13820.21642072690429098No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC12070.18901578681149003Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGT8480.13279650970682977No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG7670.12011193743530478No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG253.8888516E-545.01
TAGCGCG253.8888516E-545.01
GCGTTAG207.0309296E-445.01
TCTAGCG253.8888516E-545.01
TCTACGG351.2107921E-745.02
GATATCG253.8888516E-545.09
GTTTACG302.1639808E-644.9999961
CGTTTTT51450.043.9067041
CGTTCTG3600.043.7500041
GCGTAAG650.041.5384641
CGTTTCT4400.039.8863681
AGGGCGA3700.039.527036
CGGTCTA403.4550067E-739.37531
TAAGCGG403.4550067E-739.3752
CGTTTTC2400.039.3749961
TACGGGA2500.038.6999974
CGTAAGG950.037.8947372
AGATACG301.13935384E-437.4999961
ATGCTCG301.13935384E-437.4999961
CTCGATC301.13935384E-437.49999616
Produced by FastQC (version 0.11.5)