##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550691_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	638571
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32585413368287	33.0	31.0	34.0	30.0	34.0
2	32.5038562665702	34.0	31.0	34.0	31.0	34.0
3	32.397963578051616	34.0	31.0	34.0	30.0	34.0
4	35.96008118126254	37.0	35.0	37.0	35.0	37.0
5	36.062326663753915	37.0	35.0	37.0	35.0	37.0
6	36.11653989924378	37.0	35.0	37.0	35.0	37.0
7	36.165599439999625	37.0	37.0	37.0	35.0	37.0
8	36.09185352920819	37.0	37.0	37.0	35.0	37.0
9	37.94835186690282	39.0	38.0	39.0	35.0	39.0
10	37.73005664209618	39.0	37.0	39.0	35.0	39.0
11	37.656648986565315	39.0	37.0	39.0	35.0	39.0
12	37.654171579980925	39.0	37.0	39.0	35.0	39.0
13	37.67786667418345	39.0	37.0	39.0	35.0	39.0
14	38.99543355398225	40.0	38.0	41.0	35.0	41.0
15	39.060705857296995	40.0	38.0	41.0	35.0	41.0
16	39.075819290259034	40.0	38.0	41.0	35.0	41.0
17	39.03072798482862	40.0	38.0	41.0	35.0	41.0
18	38.87769253536412	40.0	38.0	41.0	35.0	41.0
19	38.739108102309686	40.0	37.0	41.0	35.0	41.0
20	38.59665252571758	40.0	37.0	41.0	35.0	41.0
21	38.51924688092632	40.0	37.0	41.0	35.0	41.0
22	38.49094932278478	40.0	37.0	41.0	35.0	41.0
23	38.44546495221361	40.0	37.0	41.0	35.0	41.0
24	38.416467393602275	40.0	37.0	41.0	35.0	41.0
25	38.344434683065785	40.0	37.0	41.0	35.0	41.0
26	38.35362551697462	40.0	37.0	41.0	35.0	41.0
27	38.345053251713594	40.0	37.0	41.0	35.0	41.0
28	38.25766594474224	40.0	37.0	41.0	34.0	41.0
29	38.20370326870466	40.0	37.0	41.0	34.0	41.0
30	38.07153472362509	40.0	37.0	41.0	34.0	41.0
31	37.92664558835274	40.0	36.0	41.0	34.0	41.0
32	37.699710760432275	40.0	36.0	41.0	33.0	41.0
33	37.43790588673773	40.0	36.0	41.0	33.0	41.0
34	37.20779521775965	40.0	36.0	41.0	32.0	41.0
35	37.041508305262845	40.0	36.0	41.0	31.0	41.0
36	36.93274671101569	40.0	36.0	41.0	31.0	41.0
37	36.84198937941122	40.0	36.0	41.0	31.0	41.0
38	36.777725577891886	40.0	36.0	41.0	31.0	41.0
39	36.68208233696801	40.0	35.0	41.0	30.0	41.0
40	36.58947869539957	40.0	35.0	41.0	30.0	41.0
41	36.522206614456344	40.0	35.0	41.0	30.0	41.0
42	36.442469200762325	40.0	35.0	41.0	30.0	41.0
43	36.387111221774866	40.0	35.0	41.0	30.0	41.0
44	36.296449415961575	40.0	35.0	41.0	30.0	41.0
45	36.184590280485644	39.0	35.0	41.0	29.0	41.0
46	36.112931843130994	39.0	35.0	41.0	29.0	41.0
47	36.05078683497998	39.0	35.0	41.0	28.0	41.0
48	35.99715771621323	39.0	35.0	41.0	28.0	41.0
49	35.94946215847572	39.0	35.0	41.0	28.0	41.0
50	35.87649611397949	39.0	35.0	41.0	28.0	41.0
51	34.97406553069275	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	8.0
11	13.0
12	10.0
13	15.0
14	13.0
15	16.0
16	28.0
17	45.0
18	110.0
19	211.0
20	375.0
21	601.0
22	997.0
23	1686.0
24	3007.0
25	5401.0
26	8181.0
27	9455.0
28	8979.0
29	8378.0
30	8567.0
31	9716.0
32	11786.0
33	15463.0
34	27093.0
35	39040.0
36	39622.0
37	58053.0
38	113949.0
39	267399.0
40	342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.38452106343696	20.768872999243623	23.511246204415798	19.335359732903623
2	37.18913010456159	22.958449412829584	23.752409677232443	16.10001080537638
3	29.18281600636421	22.62050735157093	32.58619636657475	15.610480275490119
4	26.129122681737815	24.408562242882937	32.268299061498254	17.194016013880994
5	24.215005065998927	27.76574570407989	29.521227866595883	18.4980213633253
6	24.148763410803184	31.82449563165255	29.00178680209405	15.024954155450216
7	74.29729818610616	5.281793253999947	14.87336568682261	5.547542873071279
8	74.71228727894001	4.902352283457908	14.933813154684444	5.451547282917639
9	68.18505694746551	7.6282825245744	17.185403032708972	7.001257495251115
10	33.192706840742844	31.572213583141107	22.17090973439132	13.064169841724727
11	24.16144798307471	23.213393655521468	33.79170053134264	18.833457830061185
12	23.132118433189106	21.59446639449646	35.148949764395816	20.124465407918617
13	21.673705821279075	21.406546805288684	37.51172539936828	19.408021974063967
14	18.685157954244712	26.314380076765154	34.61494493173038	20.385517037259756
15	18.124217980459495	26.21587889208874	36.61237356535138	19.047529562100376
16	20.31708298685659	26.59641606023449	34.75369222842879	18.332808724480127
17	20.363906284500864	26.476147523141513	32.11686719252832	21.043078999829305
18	19.85041600699061	26.678161081539876	32.96735993335119	20.504062978118327
19	20.656277845376632	27.287490349546097	31.5938243359	20.462407469177272
20	21.83312427279034	26.906483382427325	31.383510995644965	19.87688134913737
21	21.52430974785889	26.972098638992374	32.587449163836126	18.916142449312606
22	20.70357094199392	24.90921134846399	31.867717137170338	22.519500572371747
23	20.282161263195476	26.144626047847456	32.120155785339456	21.45305690361761
24	20.50312338017229	25.166034787047952	32.56364601586981	21.767195816909947
25	20.825248876005958	26.81377638508482	30.70637407586627	21.65460066304295
26	19.81972247408667	27.621517419362924	31.269663044516584	21.28909706203382
27	21.749186856277532	25.940733293557024	31.2345847211978	21.075495128967646
28	20.06777633184094	26.704000025055947	31.65051341197768	21.577710231125437
29	21.008000676510523	24.139993829973488	32.34926108451527	22.502744409000723
30	21.39151323815206	26.013552134375033	31.40465194943084	21.190282678042067
31	21.794444157345072	26.81221038850809	29.619885650929966	21.77345980321687
32	21.627195722950148	26.57965989686347	30.70997586799275	21.08316851219363
33	20.430774338327296	27.03442530274629	29.215388735160225	23.31941162376619
34	20.008581661240488	26.37639354120372	29.849773948394148	23.765250849161642
35	19.441221101490672	25.95028587267508	30.190848002806263	24.41764502302798
36	19.710729112346158	27.21811670119689	28.135164296530846	24.9359898899261
37	20.623078717949923	27.466640357924177	28.878073072532263	23.032207851593636
38	19.61974471123806	25.660419906322083	29.338945865064336	25.380889517375515
39	20.80802291366191	25.048115244820075	28.67151812406138	25.472343717456635
40	20.845450231845795	24.996593957445608	30.65391319054577	23.50404262016283
41	19.404420181937482	26.270532172616672	29.132390916593454	25.19265672885239
42	20.94724000933334	26.09388775876136	28.97124986884779	23.987622363057515
43	21.574265038656627	24.566571297475143	30.446262044471172	23.41290161939706
44	21.807441928931944	25.07990497532772	28.745746361798453	24.366906733941878
45	21.08661370466244	23.64670490830307	29.48818533882685	25.77849604820764
46	21.659298652773145	24.808361168922485	28.686551691198005	24.84578848710637
47	20.565919842899223	24.70516199451588	30.51735828905478	24.211559873530117
48	19.954711379000926	23.558539301033086	30.852324956817647	25.634424363148344
49	20.64876106180832	23.095630712951262	31.164271474902556	25.091336750337863
50	18.885448916408667	22.94827043508083	32.31167716667371	25.85460348183679
51	19.300594608900184	22.594981607370208	30.46865579551843	27.635767988211178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	289.0
1	491.5
2	694.0
3	6653.0
4	12612.0
5	8393.5
6	4175.0
7	4097.0
8	4019.0
9	3973.0
10	3927.0
11	3961.5
12	3996.0
13	3966.5
14	3937.0
15	3722.0
16	3507.0
17	3475.5
18	3444.0
19	3353.0
20	3262.0
21	3219.0
22	3176.0
23	3152.0
24	3128.0
25	3665.5
26	4772.5
27	5342.0
28	6319.5
29	7297.0
30	8318.5
31	9340.0
32	10620.5
33	11901.0
34	13361.0
35	14821.0
36	16270.5
37	17720.0
38	20016.5
39	22313.0
40	25495.5
41	28678.0
42	31364.5
43	34051.0
44	38309.0
45	42567.0
46	54094.0
47	65621.0
48	70217.0
49	74813.0
50	70206.5
51	65600.0
52	54926.5
53	44253.0
54	37674.5
55	31096.0
56	27984.0
57	24872.0
58	23005.0
59	21138.0
60	20088.5
61	19039.0
62	17317.5
63	15596.0
64	13112.5
65	10629.0
66	8465.0
67	6301.0
68	5262.0
69	4223.0
70	3560.5
71	2898.0
72	2320.5
73	1743.0
74	1385.0
75	850.5
76	674.0
77	535.0
78	396.0
79	286.0
80	176.0
81	111.0
82	46.0
83	30.5
84	15.0
85	12.0
86	9.0
87	6.5
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	638571.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.43640878317057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75877715775557	30.562988416555438
2	9.122248423845889	7.5598642942422325
3	3.794063298525146	4.7163707336073735
4	2.4732621971062976	4.099324137090365
5	1.7441093368561638	3.6134813722258268
6	1.4724713153378426	3.6608354006299018
7	1.2661446244076346	3.67251403648981
8	1.0206080731742815	3.3832266661962866
9	0.8940079542769682	3.334003114394387
>10	4.369492649190097	26.543230495374782
>50	0.052630718041056526	1.490023232994941
>100	0.02688331594468089	2.1323476344845336
>500	0.001135914758225952	0.33324305611398336
>1k	0.0037863825274198405	3.6463756436652957
>5k	3.7863825274198407E-4	1.2521717659348877
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7981	1.2498218678893966	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTT	3997	0.6259288317195738	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	3238	0.5070696915456543	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	2889	0.4524164110177255	TruSeq Adapter, Index 7 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT	2663	0.417024888383594	No Hit
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	2397	0.37536937944253657	TruSeq Adapter, Index 7 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2255	0.35313222805294947	RNA PCR Primer, Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC	1629	0.25510084234955865	No Hit
CGTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT	1584	0.24805385775426694	TruSeq Adapter, Index 7 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC	1382	0.21642072690429098	No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1207	0.18901578681149003	Illumina PCR Primer Index 3 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGT	848	0.13279650970682977	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	767	0.12011193743530478	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1319931534629666E-4	0.0	0.0	0.38319936232619395	0.0
2	3.1319931534629666E-4	0.0	0.0	1.0752132495838365	0.0
3	3.1319931534629666E-4	0.0	0.0	1.9548335267339105	0.0
4	3.1319931534629666E-4	0.0	0.0	2.8256842230542882	0.0
5	3.1319931534629666E-4	0.0	0.0	4.812464079953521	0.0
6	3.1319931534629666E-4	0.0	0.0	6.807073919736411	0.0
7	3.1319931534629666E-4	0.0	0.0	8.409245017390392	0.0
8	3.1319931534629666E-4	0.0	0.0	10.540722957979614	0.0
9	3.1319931534629666E-4	0.0	0.0	11.592759458227825	0.0
10	3.1319931534629666E-4	0.0	0.0	13.073409221527442	0.0
11	3.1319931534629666E-4	0.0	0.0	14.827795186439722	0.0
12	3.1319931534629666E-4	0.0	0.0	16.22341133562282	0.0
13	3.1319931534629666E-4	0.0	0.0	16.892718272517858	0.0
14	3.1319931534629666E-4	0.0	0.0	17.260414268734408	0.0
15	3.1319931534629666E-4	0.0	0.0	17.68245034616354	0.0
16	3.1319931534629666E-4	0.0	0.0	18.365068253960796	0.0
17	3.1319931534629666E-4	0.0	0.0	19.181892068383938	0.0
18	3.1319931534629666E-4	0.0	0.0	20.032228209549135	0.0
19	3.1319931534629666E-4	0.0	0.0	20.64390647242045	0.0
20	3.1319931534629666E-4	0.0	0.0	21.2507301459039	0.0
21	3.1319931534629666E-4	0.0	0.0	21.931938656782098	0.0
22	3.1319931534629666E-4	0.0	0.0	22.70272217184933	0.0
23	4.6979897301944497E-4	0.0	0.0	23.400843445756227	0.0
24	4.6979897301944497E-4	0.0	0.0	23.98229797469663	0.0
25	4.6979897301944497E-4	0.0	0.0	24.491090262476686	0.0
26	4.6979897301944497E-4	0.0	0.0	24.977801998524832	0.0
27	4.6979897301944497E-4	0.0	0.0	25.471560719168266	0.0
28	4.6979897301944497E-4	0.0	0.0	25.98003980763298	0.0
29	4.6979897301944497E-4	0.0	0.0	26.50778065399149	0.0
30	6.263986306925933E-4	0.0	0.0	27.120711714124194	0.0
31	6.263986306925933E-4	0.0	0.0	27.684940280720546	0.0
32	6.263986306925933E-4	0.0	0.0	28.22834109284637	0.0
33	6.263986306925933E-4	0.0	0.0	28.72225641314748	0.0
34	6.263986306925933E-4	0.0	0.0	29.222592319413188	0.0
35	6.263986306925933E-4	0.0	0.0	29.705702263334853	0.0
36	6.263986306925933E-4	0.0	0.0	30.212145556249816	0.0
37	6.263986306925933E-4	0.0	0.0	30.714517258065275	0.0
38	6.263986306925933E-4	0.0	0.0	31.20686658178965	0.0
39	6.263986306925933E-4	0.0	0.0	31.70313089695586	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.8888516E-5	45.0	1
TAGCGCG	25	3.8888516E-5	45.0	1
GCGTTAG	20	7.0309296E-4	45.0	1
TCTAGCG	25	3.8888516E-5	45.0	1
TCTACGG	35	1.2107921E-7	45.0	2
GATATCG	25	3.8888516E-5	45.0	9
GTTTACG	30	2.1639808E-6	44.999996	1
CGTTTTT	5145	0.0	43.906704	1
CGTTCTG	360	0.0	43.750004	1
GCGTAAG	65	0.0	41.538464	1
CGTTTCT	440	0.0	39.886368	1
AGGGCGA	370	0.0	39.52703	6
CGGTCTA	40	3.4550067E-7	39.375	31
TAAGCGG	40	3.4550067E-7	39.375	2
CGTTTTC	240	0.0	39.374996	1
TACGGGA	250	0.0	38.699997	4
CGTAAGG	95	0.0	37.894737	2
AGATACG	30	1.13935384E-4	37.499996	1
ATGCTCG	30	1.13935384E-4	37.499996	1
CTCGATC	30	1.13935384E-4	37.499996	16
>>END_MODULE