FastQCFastQC Report
Sat 18 Jun 2016
SRR3550690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502114
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30180.6010587237161282No Hit
CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT20480.40787550237595444No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCC15360.30590662678196584No Hit
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC14440.28758409444867106No Hit
CCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC12040.23978618401398885No Hit
CGCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTG6930.13801646638014475No Hit
TCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC5860.11670656464468228No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGC5770.1149141430033817No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTT5380.10714698255774584No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCT5370.10694782459760134No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5230.10415961315557822No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGT253.887657E-545.029
TTTCGAC207.029488E-445.024
CGCATCG253.887657E-545.021
TCTACGG207.029488E-445.02
CCAGTCG207.029488E-445.028
CCCGGGT207.029488E-445.04
CCACGCG207.029488E-445.01
CGTTTAC253.887657E-545.033
TTCGACG207.029488E-445.025
AATACGG207.029488E-445.02
AGTACGG453.8380676E-1045.02
GCCCGAC207.029488E-445.09
CCCGACG207.029488E-445.010
ATAACGG302.1630512E-644.9999962
CGTTTTT14900.042.4328841
TACGAAT850.042.3529412
GCTACGA850.042.3529410
CGTGTAC603.6379788E-1241.24999618
CGCACGG556.002665E-1140.9090922
CTACGAA900.040.011