##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550690_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	502114
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28868344638867	33.0	31.0	34.0	30.0	34.0
2	32.438165436534334	34.0	31.0	34.0	30.0	34.0
3	32.40016808931836	34.0	31.0	34.0	30.0	34.0
4	35.918849105979916	37.0	35.0	37.0	35.0	37.0
5	36.011981342882294	37.0	35.0	37.0	35.0	37.0
6	36.06190426875172	37.0	35.0	37.0	35.0	37.0
7	36.09607379997371	37.0	36.0	37.0	35.0	37.0
8	36.018683008241155	37.0	36.0	37.0	35.0	37.0
9	37.86794632294658	39.0	38.0	39.0	35.0	39.0
10	37.633043492115334	39.0	37.0	39.0	35.0	39.0
11	37.56378033673628	39.0	37.0	39.0	35.0	39.0
12	37.53257825912044	39.0	37.0	39.0	35.0	39.0
13	37.53787586086028	39.0	37.0	39.0	35.0	39.0
14	38.8273141159179	40.0	38.0	41.0	35.0	41.0
15	38.895264820339605	40.0	38.0	41.0	35.0	41.0
16	38.937920472243356	40.0	38.0	41.0	35.0	41.0
17	38.89214600668374	40.0	38.0	41.0	35.0	41.0
18	38.81818869818408	40.0	38.0	41.0	35.0	41.0
19	38.74197891315518	40.0	38.0	41.0	35.0	41.0
20	38.66031020843872	40.0	38.0	41.0	35.0	41.0
21	38.57036848205786	40.0	38.0	41.0	35.0	41.0
22	38.54528453697766	40.0	38.0	41.0	35.0	41.0
23	38.51229202930012	40.0	38.0	41.0	35.0	41.0
24	38.493182823024256	40.0	38.0	41.0	35.0	41.0
25	38.39639205439402	40.0	37.0	41.0	34.0	41.0
26	38.40777393181628	40.0	37.0	41.0	35.0	41.0
27	38.39809684653286	40.0	37.0	41.0	35.0	41.0
28	38.3348880931422	40.0	37.0	41.0	34.0	41.0
29	38.29304102255663	40.0	37.0	41.0	34.0	41.0
30	38.1987337536894	40.0	37.0	41.0	34.0	41.0
31	38.0975774425728	40.0	37.0	41.0	34.0	41.0
32	37.96405995451232	40.0	37.0	41.0	34.0	41.0
33	37.84154992690903	40.0	37.0	41.0	34.0	41.0
34	37.70950620775362	40.0	37.0	41.0	33.0	41.0
35	37.6159099328041	40.0	37.0	41.0	33.0	41.0
36	37.52369183093879	40.0	36.0	41.0	33.0	41.0
37	37.466521546899706	40.0	36.0	41.0	33.0	41.0
38	37.38752355042879	40.0	36.0	41.0	33.0	41.0
39	37.29959929418419	40.0	36.0	41.0	33.0	41.0
40	37.19786741656277	40.0	36.0	41.0	32.0	41.0
41	37.155203798340615	40.0	36.0	41.0	32.0	41.0
42	37.09752566150316	40.0	35.0	41.0	32.0	41.0
43	37.041707660013465	40.0	35.0	41.0	32.0	41.0
44	36.95469355564673	39.0	35.0	41.0	32.0	41.0
45	36.85940642961558	39.0	35.0	41.0	31.0	41.0
46	36.776479046591014	39.0	35.0	41.0	31.0	41.0
47	36.705282067418956	39.0	35.0	41.0	31.0	41.0
48	36.66498842892251	39.0	35.0	41.0	31.0	41.0
49	36.58857749435387	39.0	35.0	41.0	31.0	41.0
50	36.49538750164305	39.0	35.0	41.0	31.0	41.0
51	35.53851515791234	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	8.0
11	7.0
12	9.0
13	11.0
14	12.0
15	15.0
16	24.0
17	41.0
18	81.0
19	163.0
20	280.0
21	475.0
22	663.0
23	1130.0
24	1706.0
25	2699.0
26	3822.0
27	4471.0
28	4705.0
29	5226.0
30	5929.0
31	7506.0
32	9418.0
33	12814.0
34	23531.0
35	34088.0
36	32273.0
37	48354.0
38	94022.0
39	208350.0
40	266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.38226378870137	22.32680227996033	25.225347231903513	12.065586699434789
2	33.71505275694364	24.830217838976807	24.87980817105279	16.574921233026764
3	30.76751494680491	24.621301138785213	28.33699120120132	16.274192713208553
4	27.248194633091288	26.446982159429933	27.853634831930595	18.451188375548185
5	24.510370154984727	30.047558920882512	26.342822546274352	19.099248377858416
6	25.120789302827646	34.311929163496735	25.626053047714265	14.941228485961355
7	77.90979737669134	6.249377631374548	10.529680510800336	5.311144481133766
8	78.20236042014363	5.63816185169105	10.479691862804065	5.679785865361253
9	71.26787940587197	8.575542605862415	13.188837594649822	6.9677403936157924
10	36.10813480604006	30.839012654496788	18.94012116770295	14.112731371760198
11	26.11000689086542	25.07916528915744	29.133623041779355	19.67720477819778
12	24.42752044356461	23.253882584433015	31.66651397889722	20.65208299310515
13	22.80836622758975	23.847771621583945	33.090692551890605	20.2531695989357
14	19.446380702390297	28.158744826871985	30.571543514022714	21.823330956715008
15	18.644371596888355	27.000043814751233	34.087876458334165	20.26770813002625
16	21.072107131049922	27.366096145496837	31.256846054879965	20.30495066857327
17	20.875139908467002	27.157378603265393	29.11609714128664	22.851384346980964
18	21.380204495393475	27.179485136841436	30.159884010404014	21.280426357361076
19	21.784295996526684	28.35013562657086	28.851017896334298	21.014550480568158
20	23.499842665211485	27.963968341850652	28.410480488494645	20.125708504443214
21	22.23757951381559	28.696272161302016	29.3096786785471	19.756469646335294
22	21.043229226828966	26.010627068753312	28.26728591515074	24.678857789266978
23	20.67717689608336	28.10955281071629	28.58653612526239	22.626734167937958
24	21.31687226406752	26.493585122103745	29.63968341850655	22.549859195322178
25	20.69888511373911	28.734709647609908	27.441975328311898	23.124429910339085
26	20.03987142362093	29.353294271818754	27.705660467543225	22.90117383701709
27	21.683123752773277	28.174079989803115	28.133252607973485	22.009543649450126
28	19.27669812034717	28.577374859095745	28.618799714805803	23.527127305751282
29	20.410504387449862	27.808625132937937	27.733542581963462	24.047327897648742
30	21.779715363443362	27.54235094022473	27.48778165914513	23.190152037186774
31	21.48137673914689	29.767741986879475	25.63342189223961	23.11745938173403
32	22.599250369438014	29.65043794835436	25.3326933724214	22.417618309786224
33	21.791266525131743	27.818583030945167	26.649525804896896	23.740624639026198
34	20.271093815348706	28.16591451343719	28.3812042683534	23.181787402860703
35	21.474207052581683	26.85246776628415	27.12312343412054	24.550201747013624
36	21.187618747933737	28.545111269552333	27.057401307272848	23.20986867524108
37	21.407090820012986	28.306520033299208	28.187622731092937	22.098766415594863
38	21.732116610968824	27.277072537312243	27.429428376822795	23.56138247489614
39	23.928231437482324	24.90589786383172	29.17405210768869	21.991818590997266
40	22.23797782973588	24.407405489590015	31.49006002620919	21.864556654464923
41	20.299175087729083	25.830389114822534	28.24239117013268	25.62804462731571
42	22.840629817133163	26.69035318672652	26.544370401940597	23.924646594199725
43	22.947776799690907	25.511736378591316	28.190809258455253	23.349677563262524
44	22.0720792489355	26.14047805876753	26.970170120729552	24.817272571567415
45	22.143377798667235	24.884986278016548	28.171690094281377	24.79994582903484
46	22.512815814735298	25.564911553949898	28.123493867926406	23.798778763388395
47	20.59870865978642	25.469315733080535	29.697638384908608	24.234337222224436
48	20.462086299127293	24.67128978678149	29.603834985680543	25.26278892841068
49	22.629323221419835	23.62590965398296	29.90277108385745	23.84199604073975
50	20.800654831372956	23.2291869973751	30.453642001617165	25.516516169634784
51	20.45312419092079	22.87110098503527	28.72335764388167	27.952417180162275
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	272.0
1	512.5
2	753.0
3	2703.0
4	4653.0
5	3094.5
6	1536.0
7	1484.5
8	1433.0
9	1517.0
10	1601.0
11	1738.5
12	1876.0
13	1842.0
14	1808.0
15	1925.5
16	2043.0
17	1915.0
18	1787.0
19	1805.5
20	1824.0
21	1915.0
22	2006.0
23	2115.5
24	2225.0
25	2676.0
26	3702.0
27	4277.0
28	5032.5
29	5788.0
30	6644.0
31	7500.0
32	8363.0
33	9226.0
34	11237.5
35	13249.0
36	13999.5
37	14750.0
38	16155.0
39	17560.0
40	20024.0
41	22488.0
42	25047.0
43	27606.0
44	31715.0
45	35824.0
46	43006.5
47	50189.0
48	54982.5
49	59776.0
50	56952.5
51	54129.0
52	45547.0
53	36965.0
54	31763.0
55	26561.0
56	23334.0
57	20107.0
58	18999.5
59	17892.0
60	16751.5
61	15611.0
62	13810.0
63	12009.0
64	10123.5
65	8238.0
66	6813.5
67	5389.0
68	4393.0
69	3397.0
70	2913.5
71	2430.0
72	2181.0
73	1932.0
74	1524.0
75	867.5
76	619.0
77	435.5
78	252.0
79	193.0
80	134.0
81	118.0
82	102.0
83	59.5
84	17.0
85	12.0
86	7.0
87	16.0
88	25.0
89	13.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	502114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.339924855785085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.36041908523279	29.59353793287193
2	9.69515378609594	7.82203550392781
3	3.6405635048709972	4.405801746576288
4	2.2079090116708486	3.56267534476851
5	1.5936238687570166	3.214333355702178
6	1.2923865445669052	3.128086565547402
7	1.1101608423995266	3.1348663472162377
8	0.9450443210124417	3.0498413516022667
9	0.8705612726223694	3.1606538687948706
>10	5.184145770404566	31.862826839545065
>50	0.05992015384337603	1.6804356960741247
>100	0.03466455181014685	2.847071985308094
>500	0.0029712472980125875	0.6899934491477797
>1k	0.0024760394150104897	1.8478400129174763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3018	0.6010587237161282	No Hit
CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT	2048	0.40787550237595444	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCC	1536	0.30590662678196584	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	1444	0.28758409444867106	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	1204	0.23978618401398885	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTG	693	0.13801646638014475	No Hit
TCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	586	0.11670656464468228	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGC	577	0.1149141430033817	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTT	538	0.10714698255774584	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCT	537	0.10694782459760134	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	523	0.10415961315557822	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9915796014450903E-4	0.0	0.0	0.4180325583433244	0.0
2	1.9915796014450903E-4	0.0	0.0	1.1563111165990194	0.0
3	1.9915796014450903E-4	0.0	0.0	1.8288675480070262	0.0
4	1.9915796014450903E-4	0.0	0.0	2.6553730826067388	0.0
5	1.9915796014450903E-4	0.0	0.0	4.499575793544892	0.0
6	1.9915796014450903E-4	0.0	0.0	5.8733674026217155	0.0
7	1.9915796014450903E-4	0.0	0.0	7.108345913477816	0.0
8	1.9915796014450903E-4	0.0	0.0	9.127210155462704	0.0
9	1.9915796014450903E-4	0.0	0.0	10.012467288305047	0.0
10	1.9915796014450903E-4	0.0	0.0	11.434853439657129	0.0
11	1.9915796014450903E-4	0.0	0.0	13.505299593319446	0.0
12	1.9915796014450903E-4	0.0	0.0	15.149547712272511	0.0
13	1.9915796014450903E-4	0.0	0.0	15.852575311582628	0.0
14	1.9915796014450903E-4	0.0	0.0	16.165054151049365	0.0
15	1.9915796014450903E-4	0.0	0.0	16.609375560131763	0.0
16	1.9915796014450903E-4	0.0	0.0	17.42851225020613	0.0
17	1.9915796014450903E-4	0.0	0.0	18.43167089545402	0.0
18	1.9915796014450903E-4	0.0	0.0	19.555320106589342	0.0
19	1.9915796014450903E-4	0.0	0.0	20.324468148667435	0.0
20	1.9915796014450903E-4	0.0	0.0	21.036657014144197	0.0
21	1.9915796014450903E-4	0.0	0.0	21.87451455247215	0.0
22	1.9915796014450903E-4	0.0	0.0	22.794026854459347	0.0
23	1.9915796014450903E-4	0.0	0.0	23.68207219874371	0.0
24	1.9915796014450903E-4	0.0	0.0	24.35642105179302	0.0
25	1.9915796014450903E-4	0.0	0.0	24.960865460831602	0.0
26	1.9915796014450903E-4	0.0	0.0	25.482858474370364	0.0
27	3.9831592028901806E-4	0.0	0.0	26.03133949660834	0.0
28	3.9831592028901806E-4	0.0	0.0	26.60113042058178	0.0
29	3.9831592028901806E-4	0.0	0.0	27.201193354497185	0.0
30	3.9831592028901806E-4	0.0	0.0	27.874347259785626	0.0
31	3.9831592028901806E-4	0.0	0.0	28.488749566831437	0.0
32	3.9831592028901806E-4	0.0	0.0	29.080447866420773	0.0
33	3.9831592028901806E-4	0.0	0.0	29.624149097615284	0.0
34	3.9831592028901806E-4	0.0	0.0	30.164862959407625	0.0
35	3.9831592028901806E-4	0.0	0.0	30.724895143333985	0.0
36	3.9831592028901806E-4	0.0	0.0	31.271384585970516	0.0
37	3.9831592028901806E-4	0.0	0.0	31.831815085817166	0.0
38	3.9831592028901806E-4	0.0	0.0	32.40260179959133	0.0
39	3.9831592028901806E-4	0.0	0.0	32.94650218874598	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAGCGT	25	3.887657E-5	45.0	29
TTTCGAC	20	7.029488E-4	45.0	24
CGCATCG	25	3.887657E-5	45.0	21
TCTACGG	20	7.029488E-4	45.0	2
CCAGTCG	20	7.029488E-4	45.0	28
CCCGGGT	20	7.029488E-4	45.0	4
CCACGCG	20	7.029488E-4	45.0	1
CGTTTAC	25	3.887657E-5	45.0	33
TTCGACG	20	7.029488E-4	45.0	25
AATACGG	20	7.029488E-4	45.0	2
AGTACGG	45	3.8380676E-10	45.0	2
GCCCGAC	20	7.029488E-4	45.0	9
CCCGACG	20	7.029488E-4	45.0	10
ATAACGG	30	2.1630512E-6	44.999996	2
CGTTTTT	1490	0.0	42.432884	1
TACGAAT	85	0.0	42.35294	12
GCTACGA	85	0.0	42.35294	10
CGTGTAC	60	3.6379788E-12	41.249996	18
CGCACGG	55	6.002665E-11	40.909092	2
CTACGAA	90	0.0	40.0	11
>>END_MODULE