FastQCFastQC Report
Sat 18 Jun 2016
SRR3550689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236371
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGCT14330.6062503437392912No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC9890.41841004184100417No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC8770.37102690262341825No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCC6380.26991466804303405No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC4500.19037868435637198No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.18741723815527286No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCG4070.1721869434067631No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTC3960.16753324223360733No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGC3560.15061069251304093No Hit
ACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC3060.12945750536233294No Hit
GCCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTG2800.11845784804396478No Hit
ACCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTG2670.1129580193847807No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG207.021905E-445.00000432
GTTTACG207.021905E-445.0000041
GACGTAG207.021905E-445.0000041
TAGCCGT207.021905E-445.00000444
ACGGGCT406.7757355E-945.0000045
GTAGCCG207.021905E-445.00000443
CGAGGAC207.021905E-445.0000044
TTAGTAG207.021905E-445.0000041
GGGTTAC207.021905E-445.0000048
CGTCAAA207.021905E-445.00000412
TAATGCG207.021905E-445.0000041
GCGTAAG207.021905E-445.0000041
CGGGTAT253.8813756E-545.06
ACACGCG351.2064629E-745.036
TACGGGT253.8813756E-545.04
CGACAGG453.8198777E-1045.02
CTAACGG253.8813756E-545.02
TTACACG351.2064629E-745.034
CGTTCTG302.1581618E-644.9999961
ACGAAGG302.1581618E-644.9999962