FastQCFastQC Report
Sat 18 Jun 2016
SRR3550688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343185
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.39774465667205733No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC6130.17862086046884332No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5280.15385287818523535No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC4530.13199877617028716Illumina Single End Adapter 2 (95% over 22bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4140.1206346431225141No Hit
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT3560.10373413756428748Illumina Single End Adapter 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGT302.1610358E-645.00000412
TAATGCG302.1610358E-645.0000041
TCGTTAG207.0263643E-445.01
CACTAGG207.0263643E-445.02
TTGCGTC253.885068E-545.016
AGGGCGT207.0263643E-445.06
TACGAAT750.042.012
GCTACGA750.042.010
CGTAAGG603.6379788E-1241.2500042
TTAGTAG501.0768417E-940.51
TAGGGCG451.9215804E-840.05
CGTTTTT7950.039.9056631
TGAGTAG403.4487675E-739.3751
CTACGAA800.039.37511
CGAATAT800.039.37514
GGCGATA800.039.3758
ACGGTCT403.4487675E-739.37530
CCCTCGT1050.038.5714314
GTATTAG356.2354866E-638.5714261
TAGCGTG356.2354866E-638.5714261
Produced by FastQC (version 0.11.5)