FastQCFastQC Report
Sat 18 Jun 2016
SRR3550686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531232
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22520.42392024576832726No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT20480.385518944641889No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC17340.32641105957472444No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC14200.2673031745075598No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC14110.265608999457864No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT8210.1545464128666948No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT8060.15172278778386844No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC7440.14005180410818627No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC7250.13647521233660623No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC7130.13421631227034514No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC6380.12009818685621348No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCA207.029855E-445.0000044
GACCGCA207.029855E-445.0000041
TCTAGCG207.029855E-445.0000041
AAACGCG207.029855E-445.0000041
TATAGCG207.029855E-445.0000041
TAGGCCG207.029855E-445.0000041
GGTTCGC207.029855E-445.00000410
TCACGAC253.8879625E-545.025
CGACGGT253.8879625E-545.028
TCCGACC253.8879625E-545.029
TCGTAAG253.8879625E-545.01
CGGTCTA253.8879625E-545.031
GCGTAAG302.1632895E-644.9999961
CGTTTTT15100.041.423841
CGTATGG451.9250365E-840.02
ATATGCG356.241944E-638.571431
CGACAGG1000.038.252
CGTAAGG659.094947E-1238.076922
TATCAGG301.1390956E-437.4999962
AGCGTCG301.1390956E-437.49999617