##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550686_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	531232
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.289735934582254	33.0	31.0	34.0	30.0	34.0
2	32.44685749352448	34.0	31.0	34.0	30.0	34.0
3	32.4056739804831	34.0	31.0	34.0	30.0	34.0
4	35.91641316788145	37.0	35.0	37.0	35.0	37.0
5	36.01089919281971	37.0	35.0	37.0	35.0	37.0
6	36.051764577435094	37.0	35.0	37.0	35.0	37.0
7	36.09559665080417	37.0	36.0	37.0	35.0	37.0
8	36.0241965845431	37.0	36.0	37.0	35.0	37.0
9	37.86840401180652	39.0	38.0	39.0	35.0	39.0
10	37.612338865128606	39.0	37.0	39.0	35.0	39.0
11	37.54816163182941	39.0	37.0	39.0	35.0	39.0
12	37.55456749593398	39.0	37.0	39.0	35.0	39.0
13	37.576091048732	39.0	37.0	39.0	35.0	39.0
14	38.86719361785435	40.0	38.0	41.0	35.0	41.0
15	38.95323512137823	40.0	38.0	41.0	35.0	41.0
16	38.96913024817782	40.0	38.0	41.0	35.0	41.0
17	38.93790095476176	40.0	38.0	41.0	35.0	41.0
18	38.874205620143364	40.0	38.0	41.0	35.0	41.0
19	38.80651767965785	40.0	38.0	41.0	35.0	41.0
20	38.74085145473164	40.0	38.0	41.0	35.0	41.0
21	38.6627443376905	40.0	38.0	41.0	35.0	41.0
22	38.62367101379435	40.0	38.0	41.0	35.0	41.0
23	38.56982824829829	40.0	38.0	41.0	35.0	41.0
24	38.51644102764894	40.0	38.0	41.0	34.0	41.0
25	38.41323188362147	40.0	38.0	41.0	34.0	41.0
26	38.417484262996204	40.0	38.0	41.0	34.0	41.0
27	38.38680840009638	40.0	38.0	41.0	34.0	41.0
28	38.282981823384134	40.0	37.0	41.0	34.0	41.0
29	38.23451524004578	40.0	37.0	41.0	34.0	41.0
30	38.162249262092644	40.0	37.0	41.0	34.0	41.0
31	38.054800915607494	40.0	37.0	41.0	34.0	41.0
32	37.981239834949704	40.0	37.0	41.0	34.0	41.0
33	37.887672429371726	40.0	37.0	41.0	34.0	41.0
34	37.79070537919402	40.0	37.0	41.0	33.0	41.0
35	37.75120851153545	40.0	37.0	41.0	33.0	41.0
36	37.70318806096018	40.0	37.0	41.0	33.0	41.0
37	37.67138086561051	40.0	37.0	41.0	33.0	41.0
38	37.628853307029694	40.0	37.0	41.0	33.0	41.0
39	37.53777257394133	40.0	37.0	41.0	33.0	41.0
40	37.449125805674356	40.0	36.0	41.0	33.0	41.0
41	37.3779328052527	40.0	36.0	41.0	33.0	41.0
42	37.31510526474309	40.0	36.0	41.0	33.0	41.0
43	37.256716462863686	40.0	36.0	41.0	32.0	41.0
44	37.18437707065839	40.0	36.0	41.0	32.0	41.0
45	37.10039869586169	40.0	35.0	41.0	32.0	41.0
46	37.02184732847419	39.0	35.0	41.0	32.0	41.0
47	36.96310651466779	39.0	35.0	41.0	31.0	41.0
48	36.9328466658635	39.0	35.0	41.0	31.0	41.0
49	36.880127703150414	39.0	35.0	41.0	31.0	41.0
50	36.80049018131438	39.0	35.0	41.0	31.0	41.0
51	35.85024622010722	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	6.0
10	10.0
11	11.0
12	10.0
13	4.0
14	8.0
15	15.0
16	18.0
17	49.0
18	86.0
19	141.0
20	274.0
21	429.0
22	737.0
23	1032.0
24	1735.0
25	2610.0
26	3536.0
27	4331.0
28	4736.0
29	5248.0
30	6248.0
31	7891.0
32	9891.0
33	13336.0
34	23202.0
35	33100.0
36	33792.0
37	51054.0
38	101064.0
39	226306.0
40	317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.29042678151919	22.2863080537317	25.591455334016022	13.83180983073309
2	33.15481748087465	24.43301608336847	25.227395940003618	17.18477049575327
3	30.308791639057887	24.241009577736282	28.865354496717067	16.584844286488764
4	27.676043611830615	25.251867357388107	28.19144931028251	18.880639720498767
5	24.65834136497801	29.604203060056623	26.280419854225652	19.457035720739714
6	25.31850490934281	33.32404674417204	26.020081621589057	15.33736672489609
7	77.72611589663273	5.955778266369496	10.431976989337992	5.886128847659779
8	77.88687428468165	5.283943738329016	10.660125896030358	6.1690560809589785
9	71.01793566652611	8.164229564484067	13.44647912776339	7.371355641226432
10	34.67148063369677	31.996378230227094	19.128930486115294	14.203210649960846
11	24.691283657610988	23.935681585446662	31.759946689958436	19.613088066983916
12	23.70433257032709	21.61409704234685	33.804439491596895	20.877130895729174
13	21.86107011625806	22.825055719534966	35.07958857900127	20.23428558520571
14	18.68562134811156	27.136919462682975	32.364014216011086	21.813444973194386
15	18.29389042828745	26.55242907053792	35.00975091861936	20.143929582555266
16	20.14712969098247	26.296043913017286	32.981823384133484	20.575003011866755
17	20.34064212999217	26.74537678453105	29.76439672308897	23.149584362387806
18	20.17461297512198	26.493132943798564	31.488125715318354	21.844128365761097
19	21.101138485633395	28.217050177700138	29.506129148846455	21.175682187820012
20	22.343345280404794	27.62841846876694	29.323346485151497	20.704889765676768
21	21.594143425094874	28.25131016203843	29.869247334497924	20.285299078368773
22	20.5384841274622	25.76501415577375	29.133410637913375	24.563091078850672
23	20.11494036503825	27.54521564965966	29.254450033130535	23.085393952171557
24	20.779998192879948	25.7576727305584	30.650450274079876	22.81187880248178
25	20.197954942473345	27.473119089211494	28.104293416059274	24.224632552255887
26	19.499013613637732	28.211026444190107	29.05114902716704	23.23881091500512
27	21.573813324498524	26.101778507318834	29.216236973676285	23.108171194506355
28	19.090717426661044	26.582924221432442	29.71583037166436	24.610527980242153
29	20.16896572495633	24.24948045298476	29.063384735859287	26.518169086199627
30	20.95430998132643	24.466523101018012	30.082336907415215	24.49683001024035
31	20.20322570929462	25.551924582856454	28.024102463707006	26.22074724414192
32	19.814883139569904	25.57752545027408	28.395126799590386	26.21246461056563
33	19.559439190410217	25.004329558460338	29.281180350581295	26.15505090054816
34	17.930584000963798	24.251362869706643	30.289026263478103	27.529026865851453
35	18.241182760074697	23.75911089693392	30.31745075597855	27.682255587012833
36	18.585288536835133	26.059988856093007	29.022724534666587	26.331998072405277
37	18.49361484247937	26.291337871212576	31.18957442322752	24.025472863080537
38	18.513191976386963	25.645857177278476	29.445515330401783	26.395435515932775
39	19.887920908379016	25.41375519547015	29.554883741943254	25.143440154207582
40	20.22600295162942	23.723533220890307	31.73039274742485	24.32007108005542
41	18.421706523703392	25.604444009397024	29.74839618095296	26.22545328594663
42	20.312782362508283	25.88360640925245	29.13397536292994	24.66963586530932
43	20.648040780675863	24.797451960725258	30.801043611830615	23.753463646768267
44	20.21621438467562	24.71839045840612	29.05717276067707	26.008222396241194
45	20.307511595687007	22.446313475091863	30.261731221010784	26.98444370821035
46	21.274320824046743	23.06826396000241	29.755172881151736	25.90224233479911
47	18.968548581410758	23.376603819047045	31.952329678935005	25.702517920607193
48	19.075093367869407	22.852539003674476	31.149102463707006	26.92326516474911
49	20.043032046262272	22.549281669778928	31.718910005421357	25.68877627853744
50	18.509803626287574	22.334121438467562	32.154689476537555	27.001385458707304
51	18.43469519908439	21.609202758869948	30.344557556773687	29.611544485271974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	285.0
1	491.0
2	697.0
3	2343.5
4	3990.0
5	2744.0
6	1498.0
7	1429.5
8	1361.0
9	1417.5
10	1474.0
11	1486.0
12	1498.0
13	1484.5
14	1471.0
15	1459.0
16	1447.0
17	1418.0
18	1389.0
19	1535.0
20	1681.0
21	1879.5
22	2078.0
23	2351.5
24	2625.0
25	3269.5
26	4662.5
27	5411.0
28	6133.5
29	6856.0
30	8334.0
31	9812.0
32	10651.0
33	11490.0
34	13224.0
35	14958.0
36	15772.5
37	16587.0
38	18374.0
39	20161.0
40	22705.0
41	25249.0
42	27935.5
43	30622.0
44	33845.0
45	37068.0
46	44892.0
47	52716.0
48	57232.0
49	61748.0
50	58337.5
51	54927.0
52	46286.0
53	37645.0
54	32182.5
55	26720.0
56	24027.5
57	21335.0
58	20063.5
59	18792.0
60	17241.5
61	15691.0
62	14363.0
63	13035.0
64	11025.0
65	9015.0
66	7157.5
67	5300.0
68	4559.5
69	3819.0
70	3280.5
71	2742.0
72	2241.5
73	1741.0
74	1421.0
75	911.0
76	721.0
77	499.5
78	278.0
79	231.5
80	185.0
81	111.0
82	37.0
83	35.5
84	34.0
85	24.0
86	14.0
87	12.0
88	10.0
89	6.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	531232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.1344118109051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27001409302848	28.603800953786152
2	9.80162029772043	7.867645308856759
3	3.9873258591415226	4.800869341651706
4	2.5520097567092828	4.096936420848723
5	2.0100600066195273	4.0336288035149375
6	1.6692147082272755	4.019577030048796
7	1.3829614733220668	3.885304170224671
8	1.2348632436173814	3.9648407959592014
9	1.0688283365640694	3.860711695334434
>10	4.932547529120349	28.205262380394196
>50	0.05020389878122272	1.3810565940043829
>100	0.03331286741327054	2.3890734943014094
>500	0.004691953156798668	1.2219356746687038
>1k	0.002345976578399334	1.6693573364059269
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2252	0.42392024576832726	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	2048	0.385518944641889	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	1734	0.32641105957472444	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1420	0.2673031745075598	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1411	0.265608999457864	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	821	0.1545464128666948	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	806	0.15172278778386844	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	744	0.14005180410818627	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	725	0.13647521233660623	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	713	0.13421631227034514	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	638	0.12009818685621348	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8824167218842237E-4	0.0	0.0	0.39888410336726704	0.0
2	1.8824167218842237E-4	0.0	0.0	1.1616393590747545	0.0
3	1.8824167218842237E-4	0.0	0.0	1.8859933136558038	0.0
4	1.8824167218842237E-4	0.0	0.0	2.774682248057346	0.0
5	1.8824167218842237E-4	0.0	0.0	4.6832645623757605	0.0
6	1.8824167218842237E-4	0.0	0.0	6.266565267152581	0.0
7	1.8824167218842237E-4	0.0	0.0	7.730144268417566	0.0
8	1.8824167218842237E-4	0.0	0.0	10.086553520872236	0.0
9	1.8824167218842237E-4	0.0	0.0	11.201508945244262	0.0
10	1.8824167218842237E-4	0.0	0.0	12.746031865550268	0.0
11	1.8824167218842237E-4	0.0	0.0	14.727275465333413	0.0
12	1.8824167218842237E-4	0.0	0.0	16.301917053189566	0.0
13	1.8824167218842237E-4	0.0	0.0	16.970551472802843	0.0
14	1.8824167218842237E-4	0.0	0.0	17.283032648635626	0.0
15	1.8824167218842237E-4	0.0	0.0	17.686434552135413	0.0
16	1.8824167218842237E-4	0.0	0.0	18.39685862297452	0.0
17	1.8824167218842237E-4	0.0	0.0	19.284041623998554	0.0
18	1.8824167218842237E-4	0.0	0.0	20.288875670140353	0.0
19	1.8824167218842237E-4	0.0	0.0	20.95487470634299	0.0
20	1.8824167218842237E-4	0.0	0.0	21.606190892114935	0.0
21	1.8824167218842237E-4	0.0	0.0	22.364616589362086	0.0
22	1.8824167218842237E-4	0.0	0.0	23.183467863381725	0.0
23	3.7648334437684474E-4	0.0	0.0	23.959023552798023	0.0
24	3.7648334437684474E-4	0.0	0.0	24.567608878983194	0.0
25	3.7648334437684474E-4	0.0	0.0	25.106733028130837	0.0
26	3.7648334437684474E-4	0.0	0.0	25.60877356785736	0.0
27	3.7648334437684474E-4	0.0	0.0	26.096884223841936	0.0
28	3.7648334437684474E-4	0.0	0.0	26.62509035600265	0.0
29	3.7648334437684474E-4	0.0	0.0	27.197345039455456	0.0
30	3.7648334437684474E-4	0.0	0.0	27.835484308174205	0.0
31	3.7648334437684474E-4	0.0	0.0	28.415456900186737	0.0
32	3.7648334437684474E-4	0.0	0.0	28.94855731582435	0.0
33	5.647250165652671E-4	0.0	0.0	29.471492681163785	0.0
34	9.412083609421119E-4	0.0	0.0	29.97805102102283	0.0
35	9.412083609421119E-4	0.0	0.0	30.503433528100718	0.0
36	9.412083609421119E-4	0.0	0.0	31.007356484549124	0.0
37	9.412083609421119E-4	0.0	0.0	31.501302632371544	0.0
38	9.412083609421119E-4	0.0	0.0	32.01294349737967	0.0
39	9.412083609421119E-4	0.0	0.0	32.53625534606349	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCA	20	7.029855E-4	45.000004	4
GACCGCA	20	7.029855E-4	45.000004	1
TCTAGCG	20	7.029855E-4	45.000004	1
AAACGCG	20	7.029855E-4	45.000004	1
TATAGCG	20	7.029855E-4	45.000004	1
TAGGCCG	20	7.029855E-4	45.000004	1
GGTTCGC	20	7.029855E-4	45.000004	10
TCACGAC	25	3.8879625E-5	45.0	25
CGACGGT	25	3.8879625E-5	45.0	28
TCCGACC	25	3.8879625E-5	45.0	29
TCGTAAG	25	3.8879625E-5	45.0	1
CGGTCTA	25	3.8879625E-5	45.0	31
GCGTAAG	30	2.1632895E-6	44.999996	1
CGTTTTT	1510	0.0	41.42384	1
CGTATGG	45	1.9250365E-8	40.0	2
ATATGCG	35	6.241944E-6	38.57143	1
CGACAGG	100	0.0	38.25	2
CGTAAGG	65	9.094947E-12	38.07692	2
TATCAGG	30	1.1390956E-4	37.499996	2
AGCGTCG	30	1.1390956E-4	37.499996	17
>>END_MODULE