FastQCFastQC Report
Sat 18 Jun 2016
SRR3550681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455606
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30070.6600000877951564No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT9510.2087329842012616No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT8590.18854009824277995No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT6600.14486200796302068Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC6390.14025276225510638No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC6350.13937481069169413No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC5830.12796144036733495TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC5820.12774195247648187TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG4720.10359828448264509No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG253.8870858E-545.0000041
AGTCCGA253.8870858E-545.00000411
TTGTGCG351.2097735E-745.0000041
TTTACGG351.2097735E-745.0000042
GCGAACG253.8870858E-545.0000041
TACGAAT207.028798E-445.00000412
GTCGTGC207.028798E-445.00000422
GTCGTAG406.7993824E-945.0000041
TTGCGAG253.8870858E-545.0000041
GTCCGAA207.028798E-445.0000049
TTACGAG207.028798E-445.0000041
GCGTAAG453.8380676E-1045.0000041
CGTTTTT21750.043.7586171
TCGTAGG750.042.02
TAGGGTG1900.041.447375
CGTAAGG603.6379788E-1241.2500042
CCGCCCA501.0786607E-940.50000436
CGCCCAA501.0786607E-940.50000437
CGGGTAT451.9239451E-840.0000046
CGTGCGG451.9239451E-840.0000042
Produced by FastQC (version 0.11.5)