##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550681_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455606
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0839299745833	33.0	31.0	34.0	30.0	34.0
2	32.12132851630575	33.0	31.0	34.0	30.0	34.0
3	32.07921537468778	33.0	31.0	34.0	30.0	34.0
4	35.76872341452922	37.0	35.0	37.0	33.0	37.0
5	35.86977563947797	37.0	35.0	37.0	35.0	37.0
6	35.83153645913355	37.0	35.0	37.0	35.0	37.0
7	36.12982489256068	37.0	35.0	37.0	35.0	37.0
8	35.98665513623613	37.0	35.0	37.0	35.0	37.0
9	37.865374907266364	39.0	38.0	39.0	35.0	39.0
10	37.300371373511325	39.0	37.0	39.0	34.0	39.0
11	37.34726496139208	39.0	37.0	39.0	34.0	39.0
12	37.322625250764915	39.0	37.0	39.0	35.0	39.0
13	37.34210919083594	39.0	37.0	39.0	34.0	39.0
14	38.47752882973446	40.0	38.0	41.0	34.0	41.0
15	38.61257533921854	40.0	38.0	41.0	35.0	41.0
16	38.37015974328696	40.0	38.0	41.0	34.0	41.0
17	38.45397777904593	40.0	38.0	41.0	34.0	41.0
18	38.31922318845669	40.0	38.0	41.0	34.0	41.0
19	38.11642954658191	40.0	37.0	41.0	34.0	41.0
20	38.248598569816906	40.0	37.0	41.0	34.0	41.0
21	38.147849238157534	40.0	37.0	41.0	34.0	41.0
22	38.27496784502399	40.0	37.0	41.0	34.0	41.0
23	38.29404573249694	40.0	37.0	41.0	34.0	41.0
24	38.221623508031065	40.0	37.0	41.0	34.0	41.0
25	37.9778470871762	40.0	36.0	41.0	34.0	41.0
26	38.09338112316343	40.0	37.0	41.0	34.0	41.0
27	38.125777535853345	40.0	37.0	41.0	34.0	41.0
28	38.0452057260001	40.0	36.0	41.0	34.0	41.0
29	38.047631067194025	40.0	37.0	41.0	34.0	41.0
30	37.863425854795594	40.0	36.0	41.0	34.0	41.0
31	37.795863092233205	40.0	36.0	41.0	33.0	41.0
32	37.66153211327331	40.0	36.0	41.0	33.0	41.0
33	37.48991233653639	40.0	36.0	41.0	33.0	41.0
34	37.31417716184598	40.0	36.0	41.0	33.0	41.0
35	37.14283174497263	40.0	36.0	41.0	32.0	41.0
36	37.003096974139936	40.0	36.0	41.0	31.0	41.0
37	36.96683098993429	40.0	36.0	41.0	31.0	41.0
38	36.83369841485845	40.0	35.0	41.0	31.0	41.0
39	36.75252301330536	40.0	35.0	41.0	31.0	41.0
40	36.69406460845555	40.0	35.0	41.0	31.0	41.0
41	36.541246164449106	39.0	35.0	41.0	30.0	41.0
42	36.530943402852465	39.0	35.0	41.0	30.0	41.0
43	36.47155217446654	39.0	35.0	41.0	30.0	41.0
44	36.363221731057095	39.0	35.0	41.0	30.0	41.0
45	36.35353792531266	39.0	35.0	41.0	30.0	41.0
46	36.22519457601524	39.0	35.0	41.0	30.0	41.0
47	36.14969074156179	39.0	35.0	41.0	30.0	41.0
48	36.012883939193074	39.0	35.0	41.0	29.0	41.0
49	35.96772211077115	39.0	35.0	41.0	29.0	41.0
50	35.839014411574915	39.0	35.0	41.0	28.0	41.0
51	34.69069327445205	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	11.0
11	3.0
12	4.0
13	10.0
14	14.0
15	5.0
16	21.0
17	57.0
18	108.0
19	194.0
20	345.0
21	545.0
22	905.0
23	1324.0
24	2255.0
25	3719.0
26	5383.0
27	5764.0
28	5595.0
29	5669.0
30	6202.0
31	7205.0
32	9813.0
33	13078.0
34	23821.0
35	30928.0
36	30432.0
37	47615.0
38	92004.0
39	162495.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.39435828325351	21.330930672554796	25.400016681079705	14.874694363111987
2	34.22803913908069	23.43121030012774	25.7619522130964	16.57879834769516
3	30.223921546248295	23.070591695456162	30.864167723866675	15.841319034428873
4	26.508211041996816	25.24044898442953	30.39797544369477	17.85336452987889
5	23.298200638270785	29.35760284105126	29.271344099946006	18.072852420731948
6	23.812021790757804	33.132355587942214	28.556691527328436	14.498931093971546
7	78.84685451903619	5.523412773317296	11.212758392119508	4.416974315527012
8	81.34506569272574	4.077865524159032	10.514347923425065	4.06272085969017
9	75.63860001843699	6.552152517745596	12.649526125643648	5.159721338173773
10	37.19134515348788	25.59250756135784	22.280654776275995	14.935492508878284
11	27.9662691009337	25.554316668349408	27.060442575383114	19.418971655333774
12	24.014389626124327	23.01264689227095	31.984872894562404	20.98809058704231
13	22.85637151398357	24.002976255799968	34.154730183535776	18.985922046680685
14	20.72053484809243	26.53608600413515	32.71028037383177	20.03309877394064
15	20.19551981317191	26.37103111021365	34.897477206182536	18.535971870431908
16	22.76089428146249	25.75887938262446	32.1635360377168	19.31669029819625
17	22.839251458497035	26.12103440253201	30.282963788887766	20.756750350083188
18	23.244206617120934	25.549926910532346	31.600330109787844	19.60553636255888
19	22.4746820717901	28.930918381232907	28.752474725969368	19.841924821007627
20	22.94219127930712	27.956611633736166	29.521340807627645	19.579856279329068
21	21.794928073818166	27.947393142320337	30.943622340355482	19.31405644350601
22	21.54844317239018	25.5753875058713	30.363735332721692	22.512433989016824
23	20.407545115735964	27.203987656001015	30.536911278604762	21.85155594965826
24	20.7075850625321	26.25733638275176	31.861520699902986	21.17355785481315
25	20.71921792074731	27.846209224637075	29.74434050473435	21.69023234988126
26	20.26246361988209	27.93334591730574	30.03647888745978	21.76771157535239
27	21.168070657541822	26.767645728985134	30.131297656308302	21.932985957164743
28	19.284864554022555	26.771377023129634	30.824220927731417	23.119537495116393
29	20.35925777974829	25.666036004793614	29.927832381487512	24.04687383397058
30	21.539444168865206	25.123461938604848	29.552947063910484	23.784146828619466
31	21.012014767145295	25.88925518979118	28.457702488553704	24.64102755450982
32	20.66741877850599	25.45137684753932	29.398208100859076	24.482996273095615
33	20.78769814269347	25.450937871757617	29.64096170814256	24.120402277406356
34	20.107944144721536	25.15309280387001	30.497622946142062	24.241340105266392
35	20.157328920163476	25.772926607639057	30.842657910563076	23.227086561634394
36	20.722510239110107	27.108290935589086	29.116824624785448	23.05237420051536
37	20.479756631826625	27.45771565782716	29.727439937138666	22.33508777320755
38	19.690039200537306	27.426767865216878	30.65741013068309	22.225782803562726
39	21.2523540076294	26.683142891006707	29.861766526340745	22.202736575023156
40	22.57498803790995	24.98233122478633	30.235334916572654	22.20734582073107
41	20.135160643187316	26.365104937160616	29.388111657879833	24.11162276177223
42	20.488755635351595	26.57844716706979	29.477004253675325	23.455792943903283
43	20.343893627388574	25.550585374204903	30.150173614921666	23.955347383484852
44	20.54757839010022	25.85194224834616	28.777276857635766	24.82320250391786
45	20.736118488342996	24.893877604772545	29.254882508132024	25.115121398752432
46	21.83904513987963	25.12785169642191	28.61836762465815	24.41473553904031
47	20.220980408510865	25.587239851977365	30.01145726790253	24.18032247160924
48	20.237442000324844	24.964113729845526	30.440775582411117	24.357668687418517
49	21.199676913824664	24.018120920268828	30.568078559105892	24.214123606800612
50	19.71330491696773	23.918912393603247	31.175621040987167	25.192161648441857
51	19.74030192754266	23.67989008046426	29.696711632419238	26.883096359573845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	262.0
2	378.0
3	2774.0
4	5170.0
5	3573.5
6	1977.0
7	1992.5
8	2008.0
9	2076.5
10	2145.0
11	2138.0
12	2131.0
13	2124.5
14	2118.0
15	2063.0
16	2008.0
17	1964.5
18	1921.0
19	1817.0
20	1713.0
21	1842.0
22	1971.0
23	2071.5
24	2172.0
25	2563.5
26	3356.5
27	3758.0
28	4447.5
29	5137.0
30	6037.0
31	6937.0
32	8265.0
33	9593.0
34	10925.0
35	12257.0
36	13329.5
37	14402.0
38	16101.5
39	17801.0
40	19526.5
41	21252.0
42	24158.5
43	27065.0
44	30000.0
45	32935.0
46	38322.5
47	43710.0
48	45973.5
49	48237.0
50	46422.0
51	44607.0
52	39396.0
53	34185.0
54	30025.5
55	25866.0
56	23194.5
57	20523.0
58	18376.0
59	16229.0
60	14701.0
61	13173.0
62	11553.5
63	9934.0
64	8461.0
65	6988.0
66	5538.0
67	4088.0
68	3666.0
69	3244.0
70	2529.5
71	1815.0
72	1513.5
73	1212.0
74	1024.5
75	686.5
76	536.0
77	389.5
78	243.0
79	192.0
80	141.0
81	103.0
82	65.0
83	36.0
84	7.0
85	9.0
86	11.0
87	7.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	455606.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.15187296120323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.05123874296267	32.98400251380777
2	9.218755047976176	8.324881135733426
3	4.1542635438313065	5.627183393352871
4	2.7143563219047584	4.902330872723301
5	2.029688137032013	4.5822110457065355
6	1.6621252824485024	4.502884175923129
7	1.3245249709768698	4.186334825644232
8	1.092009630907284	3.9445024101708888
9	0.9004933546654693	3.6593065397036697
>10	3.810116782674575	23.77004067813845
>50	0.029748497262632678	0.9127156607019077
>100	0.00877824509384205	0.860529023403498
>500	0.0034137619809385743	1.0809460020183654
>1k	4.876802829912249E-4	0.662131722971934
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3007	0.6600000877951564	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	951	0.2087329842012616	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	859	0.18854009824277995	No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	660	0.14486200796302068	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	639	0.14025276225510638	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC	635	0.13937481069169413	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	583	0.12796144036733495	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	582	0.12774195247648187	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	472	0.10359828448264509	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1948789085306163E-4	0.0	0.0	0.15539742672396764	0.0
2	2.1948789085306163E-4	0.0	0.0	0.548719727132654	0.0
3	2.1948789085306163E-4	0.0	0.0	0.8498571133830547	0.0
4	2.1948789085306163E-4	0.0	0.0	1.2965149712690351	0.0
5	2.1948789085306163E-4	0.0	0.0	2.53135384520836	0.0
6	2.1948789085306163E-4	0.0	0.0	3.8122851762268275	0.0
7	2.1948789085306163E-4	0.0	0.0	4.899408699622042	0.0
8	2.1948789085306163E-4	0.0	0.0	6.427922371522763	0.0
9	2.1948789085306163E-4	0.0	0.0	7.104823026913605	0.0
10	2.1948789085306163E-4	0.0	0.0	8.27513246094213	0.0
11	2.1948789085306163E-4	0.0	0.0	9.712997633920537	0.0
12	2.1948789085306163E-4	0.0	0.0	10.787390859646273	0.0
13	2.1948789085306163E-4	0.0	0.0	11.287384275009547	0.0
14	2.1948789085306163E-4	0.0	0.0	11.524211709240001	0.0
15	4.3897578170612326E-4	0.0	0.0	11.81656957985628	0.0
16	4.3897578170612326E-4	0.0	0.0	12.387457583965093	0.0
17	4.3897578170612326E-4	0.0	0.0	13.14139848904536	0.0
18	4.3897578170612326E-4	0.0	0.0	13.949333415275479	0.0
19	4.3897578170612326E-4	0.0	0.0	14.450863245874725	0.0
20	4.3897578170612326E-4	0.0	0.0	14.962928495234918	0.0
21	4.3897578170612326E-4	0.0	0.0	15.598565427145385	0.0
22	4.3897578170612326E-4	0.0	0.0	16.30729182670992	0.0
23	4.3897578170612326E-4	0.0	0.0	17.042576261067676	0.0
24	4.3897578170612326E-4	0.0	0.0	17.613244777285637	0.0
25	4.3897578170612326E-4	0.0	0.0	18.13825981220616	0.0
26	4.3897578170612326E-4	0.0	0.0	18.63627783655176	0.0
27	4.3897578170612326E-4	0.0	0.0	19.14175844918636	0.0
28	6.584636725591849E-4	0.0	0.0	19.662822702071526	0.0
29	6.584636725591849E-4	0.0	0.0	20.210006013968208	0.0
30	6.584636725591849E-4	0.0	0.0	20.85091065525915	0.0
31	6.584636725591849E-4	0.0	0.0	21.413458119515546	0.0
32	6.584636725591849E-4	0.0	0.0	21.9584465525037	0.0
33	6.584636725591849E-4	0.0	0.0	22.50760525541806	0.0
34	6.584636725591849E-4	0.0	0.0	23.034156705574553	0.0
35	6.584636725591849E-4	0.0	0.0	23.564878425657255	0.0
36	6.584636725591849E-4	0.0	0.0	24.071017501964416	0.0
37	6.584636725591849E-4	0.0	0.0	24.60678744353674	0.0
38	6.584636725591849E-4	0.0	0.0	25.16735951677546	0.0
39	6.584636725591849E-4	0.0	0.0	25.806069279157867	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	25	3.8870858E-5	45.000004	1
AGTCCGA	25	3.8870858E-5	45.000004	11
TTGTGCG	35	1.2097735E-7	45.000004	1
TTTACGG	35	1.2097735E-7	45.000004	2
GCGAACG	25	3.8870858E-5	45.000004	1
TACGAAT	20	7.028798E-4	45.000004	12
GTCGTGC	20	7.028798E-4	45.000004	22
GTCGTAG	40	6.7993824E-9	45.000004	1
TTGCGAG	25	3.8870858E-5	45.000004	1
GTCCGAA	20	7.028798E-4	45.000004	9
TTACGAG	20	7.028798E-4	45.000004	1
GCGTAAG	45	3.8380676E-10	45.000004	1
CGTTTTT	2175	0.0	43.758617	1
TCGTAGG	75	0.0	42.0	2
TAGGGTG	190	0.0	41.44737	5
CGTAAGG	60	3.6379788E-12	41.250004	2
CCGCCCA	50	1.0786607E-9	40.500004	36
CGCCCAA	50	1.0786607E-9	40.500004	37
CGGGTAT	45	1.9239451E-8	40.000004	6
CGTGCGG	45	1.9239451E-8	40.000004	2
>>END_MODULE