SRR3550680_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550680_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294052
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	681	0.23159169126549048	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.20370546706024784	No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	463	0.15745514398813815	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	379	0.12888876797301158	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	345	0.11732618720498415	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC	317	0.10780406186660862	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGCTT	25	3.883702E-5	45.000004	6
CCAAGCG	25	3.883702E-5	45.000004	20
TGCACCA	25	3.883702E-5	45.000004	28
TTGGACG	20	7.0247165E-4	45.0	1
ATAGACG	20	7.0247165E-4	45.0	1
CGGATTA	20	7.0247165E-4	45.0	6
TGGGACG	35	1.207809E-7	45.0	6
TATGCGG	30	2.1599735E-6	44.999996	2
CACAACG	60	0.0	44.999996	12
ATCGCGG	30	2.1599735E-6	44.999996	2
ATCGATG	30	2.1599735E-6	44.999996	1
TAGGGCG	85	0.0	42.35294	5
GCGATGC	55	6.002665E-11	40.909092	9
AGGGTAC	105	0.0	40.714287	6
GTGCAAG	50	1.0750227E-9	40.500004	1
TACGAAT	50	1.0750227E-9	40.500004	12
CGTTTTT	450	0.0	40.0	1
CGTGTAC	40	3.4465302E-7	39.375	18
ACAACGA	70	0.0	38.571426	13
ACGGGCT	70	0.0	38.571426	5