##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550680_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294052
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10471277189069	33.0	31.0	34.0	30.0	34.0
2	32.11221484635371	33.0	31.0	34.0	30.0	34.0
3	32.13319752968863	33.0	31.0	34.0	30.0	34.0
4	35.79109817311224	37.0	35.0	37.0	35.0	37.0
5	35.878643913321454	37.0	35.0	37.0	35.0	37.0
6	35.832084121175846	37.0	35.0	37.0	35.0	37.0
7	36.13179641696026	37.0	35.0	37.0	35.0	37.0
8	35.96495177723668	37.0	35.0	37.0	35.0	37.0
9	37.8300368642281	39.0	38.0	39.0	35.0	39.0
10	37.38489110769524	39.0	37.0	39.0	34.0	39.0
11	37.366907893841905	39.0	37.0	39.0	34.0	39.0
12	37.2763762871873	39.0	37.0	39.0	34.0	39.0
13	37.23771645831349	39.0	37.0	39.0	34.0	39.0
14	38.31582849291962	40.0	38.0	41.0	34.0	41.0
15	38.46635288996504	40.0	38.0	41.0	34.0	41.0
16	38.25799178376614	40.0	37.0	41.0	34.0	41.0
17	38.33740290832914	40.0	37.0	41.0	34.0	41.0
18	38.27194169738686	40.0	37.0	41.0	34.0	41.0
19	38.13048032320814	40.0	37.0	41.0	34.0	41.0
20	38.318487886496264	40.0	37.0	41.0	34.0	41.0
21	38.20808904547495	40.0	37.0	41.0	34.0	41.0
22	38.32689116210738	40.0	37.0	41.0	34.0	41.0
23	38.355233088025244	40.0	37.0	41.0	34.0	41.0
24	38.294277882823444	40.0	37.0	41.0	34.0	41.0
25	38.036554759022216	40.0	36.0	41.0	34.0	41.0
26	38.15509841796689	40.0	37.0	41.0	34.0	41.0
27	38.17817596887625	40.0	37.0	41.0	34.0	41.0
28	38.11746561832601	40.0	37.0	41.0	34.0	41.0
29	38.15851618081156	40.0	37.0	41.0	34.0	41.0
30	37.97051541904153	40.0	36.0	41.0	34.0	41.0
31	37.94246935916097	40.0	36.0	41.0	34.0	41.0
32	37.91759620747351	40.0	36.0	41.0	34.0	41.0
33	37.87044808401235	40.0	36.0	41.0	34.0	41.0
34	37.78098771645831	40.0	36.0	41.0	34.0	41.0
35	37.70257641505584	40.0	36.0	41.0	33.0	41.0
36	37.59417722035558	40.0	36.0	41.0	33.0	41.0
37	37.55790472433447	40.0	36.0	41.0	33.0	41.0
38	37.43305265735312	40.0	36.0	41.0	33.0	41.0
39	37.361813556785876	40.0	35.0	41.0	33.0	41.0
40	37.27991987811679	40.0	35.0	41.0	33.0	41.0
41	37.15565954321004	40.0	35.0	41.0	32.0	41.0
42	37.15767619332635	40.0	35.0	41.0	32.0	41.0
43	37.07394950552963	39.0	35.0	41.0	32.0	41.0
44	36.96235699808197	39.0	35.0	41.0	32.0	41.0
45	36.93652143158353	39.0	35.0	41.0	32.0	41.0
46	36.800202005087534	39.0	35.0	41.0	32.0	41.0
47	36.73511827839974	39.0	35.0	41.0	31.0	41.0
48	36.643933726007646	39.0	35.0	41.0	31.0	41.0
49	36.60745378368452	39.0	35.0	41.0	31.0	41.0
50	36.49757525879777	39.0	35.0	41.0	31.0	41.0
51	35.336889393712674	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	6.0
11	5.0
12	3.0
13	4.0
14	1.0
15	13.0
16	14.0
17	35.0
18	56.0
19	130.0
20	167.0
21	278.0
22	429.0
23	658.0
24	1012.0
25	1429.0
26	1808.0
27	2252.0
28	2540.0
29	3045.0
30	3677.0
31	4712.0
32	6070.0
33	8756.0
34	16749.0
35	22926.0
36	20146.0
37	31026.0
38	59069.0
39	106973.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.02063580591188	21.689361065389797	26.20250839987485	11.087494728823472
2	31.40703004910696	24.66366493001238	26.190945819106826	17.738359201773836
3	31.457361283038377	24.38956375062914	27.581176118509653	16.571898847822833
4	28.297375974317468	26.239236597608585	26.43341993933046	19.029967488743488
5	24.73576102186008	31.126807503434765	25.17309863561547	18.964332839089685
6	25.70837810999415	35.55731639301892	23.790009930216424	14.944295566770501
7	81.45906166256309	6.327452287350536	7.530640839035272	4.682845211051107
8	82.80202141117897	5.073252349924503	7.503434766639915	4.621291472256607
9	76.58679417245929	7.9397521526804775	9.844177220355583	5.629276454504645
10	39.82152816508645	28.904071388733964	16.917075891338946	14.35732455484064
11	29.82363663569709	25.560105015439444	25.06291404241427	19.5533443064492
12	26.487832084121177	23.631534558513458	29.396161223185015	20.484472134180347
13	24.10798090133718	25.21288751649368	30.739461047705845	19.939670534463293
14	19.97707888400691	28.017493504550217	30.886033762735842	21.119393848707034
15	19.38058574673867	26.46980806115925	34.25686613252078	19.8927400595813
16	22.781344796158503	25.74748683906248	30.647980629276457	20.823187735502565
17	22.576279025478488	26.63066396419681	27.93417490783943	22.858882102485275
18	23.447553493939846	25.68219226531362	30.033803544951233	20.836450695795303
19	23.979092133364166	28.127678097751417	26.89184225919225	21.00138750969216
20	25.31320990845157	27.764477031273383	27.136356834845536	19.785956225429516
21	23.116999714336238	28.23786269095262	28.717709792825758	19.92742780188538
22	22.104593745323957	25.51215431284263	28.322541591283173	24.060710350550245
23	21.595500115625807	27.269666589582798	28.530327969202723	22.604505325588672
24	21.61454436630256	26.04437310407683	29.962047529008473	22.379035000612134
25	21.29929400242134	27.85391699427312	27.950838627181586	22.89595037612395
26	20.85175411151769	28.23208140056861	27.98518629358073	22.930978194332972
27	21.039476011045664	27.607702039095127	29.265231999782355	22.087589950076858
28	18.317168391985092	28.281392406785194	30.031082937711695	23.370356263518016
29	20.547726252499558	26.390230299402827	28.6629575721301	24.399085875967515
30	21.36118781712078	27.062220287568184	29.05064410376396	22.525947791547075
31	21.86484023233986	28.19807381007441	26.574211363976442	23.36287459360929
32	22.7157101465047	27.108130534735352	27.26218491967407	22.913974399085877
33	21.732550705317426	27.88112306666848	26.994545182484732	23.39178104552936
34	19.51389550147593	27.585597105273894	28.498700910043123	24.401806483207054
35	20.684776842191177	26.463346618965357	28.757498673703967	24.0943778651395
36	21.360507665310895	28.895569491110418	26.37526695958538	23.36865588399331
37	20.57051133813067	28.34566675281923	28.45959218097479	22.624229728075306
38	21.12755567042564	28.943180117802292	27.001686776488516	22.927577435283556
39	21.289431801178026	26.573191136261613	27.244160896712145	24.893216165848216
40	22.99525254036701	25.779794050031967	27.460449172255245	23.764504237345776
41	20.6922585120999	25.448220042713533	26.81430495286548	27.045216492321085
42	21.46185028498361	24.87179138383687	28.200114265504062	25.466244065675458
43	21.95087943629018	25.15099370179424	28.6734999251833	24.224626936732278
44	20.63920667092895	26.62726320514739	27.660413804361134	25.073116319562526
45	20.875559424863628	25.654306041108377	27.73046944077918	25.739665093248814
46	21.9607416375335	26.637465482295646	26.961217743800415	24.440575136370438
47	19.930828560934803	26.03995211731258	29.312162474664344	24.71705684708827
48	20.948675744426158	25.17445893923524	28.783004366574623	25.093860949763986
49	21.399616394379226	24.45621862799777	29.829758001986047	24.314406975636963
50	19.56558703902711	24.98503666018255	29.50872634772081	25.940649953069528
51	19.974358276767372	24.376980942146286	27.080584386435053	28.568076394651285
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	203.0
2	304.0
3	727.0
4	1150.0
5	846.5
6	543.0
7	541.0
8	539.0
9	555.5
10	572.0
11	625.5
12	679.0
13	675.5
14	672.0
15	682.0
16	692.0
17	656.0
18	620.0
19	723.0
20	826.0
21	937.5
22	1049.0
23	1104.5
24	1160.0
25	1395.0
26	2032.5
27	2435.0
28	3144.0
29	3853.0
30	4519.5
31	5186.0
32	5664.5
33	6143.0
34	6997.0
35	7851.0
36	8753.5
37	9656.0
38	10684.5
39	11713.0
40	13159.0
41	14605.0
42	16297.5
43	17990.0
44	20201.0
45	22412.0
46	24839.0
47	27266.0
48	29056.5
49	30847.0
50	29916.0
51	28985.0
52	24990.0
53	20995.0
54	18158.0
55	15321.0
56	14174.5
57	13028.0
58	12050.5
59	11073.0
60	10316.5
61	9560.0
62	8571.5
63	7583.0
64	6606.0
65	5629.0
66	4658.5
67	3688.0
68	3272.0
69	2856.0
70	2303.0
71	1750.0
72	1503.0
73	1256.0
74	1107.0
75	671.5
76	385.0
77	315.5
78	246.0
79	184.5
80	123.0
81	83.5
82	44.0
83	50.5
84	57.0
85	31.0
86	5.0
87	8.0
88	11.0
89	6.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	294052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.04765404470058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5954026517748	29.38823319241091
2	9.133240742881279	7.497962126415097
3	3.9075920603471546	4.811924611228463
4	2.526349561586795	4.14802891199983
5	1.925196591899815	3.951240183617011
6	1.6186318367698536	3.9864623796880334
7	1.3934114893892897	4.003739093085378
8	1.2646668573432598	4.152928611361987
9	1.106057971605353	4.086097647465581
>10	5.4704937966817875	31.27113723767162
>50	0.04484010908329346	1.2181921319463294
>100	0.012455585856470407	1.0477679388928418
>500	0.0016607447808627209	0.43628593421692835
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	681	0.23159169126549048	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.20370546706024784	No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	463	0.15745514398813815	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	379	0.12888876797301158	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	345	0.11732618720498415	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC	317	0.10780406186660862	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4007590494198307E-4	0.0	0.0	0.16595704161168773	0.0
2	3.4007590494198307E-4	0.0	0.0	0.7512276740168405	0.0
3	3.4007590494198307E-4	0.0	0.0	1.0328105233088025	0.0
4	3.4007590494198307E-4	0.0	0.0	1.4701481370641927	0.0
5	3.4007590494198307E-4	0.0	0.0	2.7746793084216397	0.0
6	3.4007590494198307E-4	0.0	0.0	3.6238488430617712	0.0
7	3.4007590494198307E-4	0.0	0.0	4.540013330975474	0.0
8	3.4007590494198307E-4	0.0	0.0	6.047909893488226	0.0
9	3.4007590494198307E-4	0.0	0.0	6.6151565029314545	0.0
10	3.4007590494198307E-4	0.0	0.0	7.792159209935658	0.0
11	3.4007590494198307E-4	0.0	0.0	9.412280821079264	0.0
12	3.4007590494198307E-4	0.0	0.0	10.553915633969503	0.0
13	3.4007590494198307E-4	0.0	0.0	10.999074993538558	0.0
14	3.4007590494198307E-4	0.0	0.0	11.17625454001333	0.0
15	3.4007590494198307E-4	0.0	0.0	11.488444220750072	0.0
16	3.4007590494198307E-4	0.0	0.0	12.19648225483928	0.0
17	3.4007590494198307E-4	0.0	0.0	13.103464693319548	0.0
18	3.4007590494198307E-4	0.0	0.0	14.094445880320487	0.0
19	3.4007590494198307E-4	0.0	0.0	14.692979473018378	0.0
20	6.801518098839661E-4	0.0	0.0	15.285731775332254	0.0
21	6.801518098839661E-4	0.0	0.0	16.00839307333397	0.0
22	6.801518098839661E-4	0.0	0.0	16.76132112687552	0.0
23	6.801518098839661E-4	0.0	0.0	17.517649939466487	0.0
24	6.801518098839661E-4	0.0	0.0	18.122304898453336	0.0
25	6.801518098839661E-4	0.0	0.0	18.64806224749364	0.0
26	6.801518098839661E-4	0.0	0.0	19.14695360004353	0.0
27	6.801518098839661E-4	0.0	0.0	19.67237087317889	0.0
28	6.801518098839661E-4	0.0	0.0	20.183845034211636	0.0
29	6.801518098839661E-4	0.0	0.0	20.739529062886838	0.0
30	6.801518098839661E-4	0.0	0.0	21.379891991892592	0.0
31	6.801518098839661E-4	0.0	0.0	21.909050099982316	0.0
32	6.801518098839661E-4	0.0	0.0	22.4378681321671	0.0
33	6.801518098839661E-4	0.0	0.0	22.9724674547359	0.0
34	6.801518098839661E-4	0.0	0.0	23.511147688163998	0.0
35	6.801518098839661E-4	0.0	0.0	24.038605416729013	0.0
36	0.0010202277148259492	0.0	0.0	24.548719274141988	0.0
37	0.0010202277148259492	0.0	0.0	25.055432372505543	0.0
38	0.0010202277148259492	0.0	0.0	25.59309237821882	0.0
39	0.0010202277148259492	0.0	0.0	26.19910764082543	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGCTT	25	3.883702E-5	45.000004	6
CCAAGCG	25	3.883702E-5	45.000004	20
TGCACCA	25	3.883702E-5	45.000004	28
TTGGACG	20	7.0247165E-4	45.0	1
ATAGACG	20	7.0247165E-4	45.0	1
CGGATTA	20	7.0247165E-4	45.0	6
TGGGACG	35	1.207809E-7	45.0	6
TATGCGG	30	2.1599735E-6	44.999996	2
CACAACG	60	0.0	44.999996	12
ATCGCGG	30	2.1599735E-6	44.999996	2
ATCGATG	30	2.1599735E-6	44.999996	1
TAGGGCG	85	0.0	42.35294	5
GCGATGC	55	6.002665E-11	40.909092	9
AGGGTAC	105	0.0	40.714287	6
GTGCAAG	50	1.0750227E-9	40.500004	1
TACGAAT	50	1.0750227E-9	40.500004	12
CGTTTTT	450	0.0	40.0	1
CGTGTAC	40	3.4465302E-7	39.375	18
ACAACGA	70	0.0	38.571426	13
ACGGGCT	70	0.0	38.571426	5
>>END_MODULE