FastQCFastQC Report
Sat 18 Jun 2016
SRR3550677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260059
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC14700.5652563456754045No Hit
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT11450.44028470462471975Illumina Single End Adapter 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC10200.3922186888359949No Hit
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC9570.3679934168784776No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGC8710.33492399801583483No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.3176202323318939No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCG8140.31300589481617636No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC7010.269554216543169No Hit
TCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC6640.2553266758697065No Hit
ACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC4400.16919237557631153No Hit
CGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG4390.16880784745000174No Hit
ACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG3570.13727654109259824No Hit
AGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG3200.12304900041913566No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCT3180.12227994416651605No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC3140.12074183166127686No Hit
GCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG2860.1099750441246025No Hit
GGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG2650.10189995347209672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT207.023209E-445.00000428
TAAGTAG207.023209E-445.0000041
AGGCGAT207.023209E-445.0000046
TATGCGC207.023209E-445.00000422
AACGGGC207.023209E-445.0000044
GCCGATG207.023209E-445.0000049
TGCGCGC207.023209E-445.00000415
AGCGATG207.023209E-445.0000041
CTATTAG207.023209E-445.0000041
GTAAGCG207.023209E-445.0000041
CCTGCGT207.023209E-445.00000423
TACGTAG207.023209E-445.0000041
CCTCGTC351.2070814E-745.036
CTACGAA351.2070814E-745.011
TCGTCCC351.2070814E-745.038
GCTACGA351.2070814E-745.010
CCTATGC351.2070814E-745.035
CAACGAG302.159004E-644.99999614
GCGCGAC253.8824568E-544.9999969
TGATTCA302.159004E-644.99999644