##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550677_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260059
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.138722366847524	33.0	31.0	34.0	30.0	34.0
2	32.285858209098706	34.0	31.0	34.0	30.0	34.0
3	32.26310183458369	34.0	31.0	34.0	30.0	34.0
4	35.808797234473715	37.0	35.0	37.0	35.0	37.0
5	35.92273676358057	37.0	35.0	37.0	35.0	37.0
6	35.97539404519743	37.0	35.0	37.0	35.0	37.0
7	36.04414382890037	37.0	35.0	37.0	35.0	37.0
8	35.9396982992321	37.0	35.0	37.0	35.0	37.0
9	37.8037137726439	39.0	38.0	39.0	35.0	39.0
10	37.454312290672505	39.0	37.0	39.0	35.0	39.0
11	37.43062535809182	39.0	37.0	39.0	35.0	39.0
12	37.41149508380791	39.0	37.0	39.0	35.0	39.0
13	37.40938402439446	39.0	37.0	39.0	35.0	39.0
14	38.63525584578884	40.0	38.0	41.0	35.0	41.0
15	38.70517074971449	40.0	38.0	41.0	35.0	41.0
16	38.75988910208837	40.0	38.0	41.0	35.0	41.0
17	38.72386650721567	40.0	38.0	41.0	35.0	41.0
18	38.66950961128052	40.0	38.0	41.0	35.0	41.0
19	38.61782903110448	40.0	38.0	41.0	35.0	41.0
20	38.537989456238776	40.0	38.0	41.0	34.0	41.0
21	38.485220661465284	40.0	38.0	41.0	34.0	41.0
22	38.46457534636371	40.0	38.0	41.0	34.0	41.0
23	38.43842358849338	40.0	38.0	41.0	34.0	41.0
24	38.39066134992444	40.0	37.0	41.0	34.0	41.0
25	38.3127636420966	40.0	37.0	41.0	34.0	41.0
26	38.362490819390985	40.0	37.0	41.0	34.0	41.0
27	38.36431732799095	40.0	37.0	41.0	34.0	41.0
28	38.28957659608012	40.0	37.0	41.0	34.0	41.0
29	38.2562341622478	40.0	37.0	41.0	34.0	41.0
30	38.15745273188007	40.0	37.0	41.0	34.0	41.0
31	38.06934195701744	40.0	37.0	41.0	34.0	41.0
32	37.968130308891446	40.0	37.0	41.0	34.0	41.0
33	37.86116227471459	40.0	37.0	41.0	34.0	41.0
34	37.759366143836594	40.0	37.0	41.0	33.0	41.0
35	37.70170230601517	40.0	37.0	41.0	33.0	41.0
36	37.603324630180076	40.0	37.0	41.0	33.0	41.0
37	37.545449301889185	40.0	36.0	41.0	33.0	41.0
38	37.45648487458615	40.0	36.0	41.0	33.0	41.0
39	37.36855867322415	40.0	36.0	41.0	33.0	41.0
40	37.2894535470797	40.0	36.0	41.0	32.0	41.0
41	37.24081073910151	40.0	36.0	41.0	32.0	41.0
42	37.213647672258986	40.0	36.0	41.0	32.0	41.0
43	37.14600917484109	40.0	36.0	41.0	32.0	41.0
44	37.07468689797315	39.0	35.0	41.0	32.0	41.0
45	36.98658381367305	39.0	35.0	41.0	31.0	41.0
46	36.885979719986615	39.0	35.0	41.0	31.0	41.0
47	36.75810104630103	39.0	35.0	41.0	31.0	41.0
48	36.743508203907574	39.0	35.0	41.0	31.0	41.0
49	36.705712934372585	39.0	35.0	41.0	31.0	41.0
50	36.58156802879346	39.0	35.0	41.0	31.0	41.0
51	35.53666667948427	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	2.0
10	5.0
11	6.0
12	5.0
13	1.0
14	3.0
15	4.0
16	10.0
17	18.0
18	34.0
19	55.0
20	111.0
21	193.0
22	312.0
23	527.0
24	832.0
25	1242.0
26	1593.0
27	1930.0
28	2262.0
29	2602.0
30	3286.0
31	4115.0
32	5385.0
33	7382.0
34	12878.0
35	18011.0
36	17573.0
37	27084.0
38	52127.0
39	100375.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.166043090221834	23.2351120322696	24.186434616760046	11.412410260748523
2	32.10732949061559	25.34078805194206	25.234273760954245	17.317608696488104
3	30.017803652248144	25.16159794508169	27.761008078935934	17.05959032373423
4	26.970802779369297	25.128913054345357	29.306811146701328	18.593473019584017
5	24.515590692881233	29.406019403289253	26.33402420220027	19.744365701629246
6	24.30756097654763	33.65851595214932	26.490527149608354	15.543395921694692
7	76.71989817695216	5.656024209890832	11.706958805501829	5.917118807655186
8	75.93046193363814	5.752540769594592	11.483547964115836	6.833449332651437
9	70.00872878846724	7.260275552855314	13.439642542653782	9.291353116023672
10	36.60900026532441	25.195436420196955	22.20573023813827	15.989833076340368
11	26.26404008321189	24.454066192671664	26.933119022990937	22.348774701125514
12	23.3685432920991	21.97501336235239	32.687582433217074	21.968860912331433
13	22.635632683352625	23.710773324514822	34.93476480337154	18.718829188761013
14	19.834729811312048	28.24666710246521	30.926059086591888	20.992543999630854
15	18.557711903837205	25.962954560311314	35.755347824916655	19.723985710934826
16	20.028916515098498	26.889667344717928	30.804548198677995	22.276867941505582
17	20.101592330971048	27.02386766080005	28.518528487766236	24.356011520462665
18	20.42574954145021	27.020406907663265	31.129474465409775	21.42436908547676
19	20.46343329782857	28.686182750837308	28.372792327894825	22.477591623439295
20	21.446671716802726	28.559288469155074	29.787086776462264	20.206953037579932
21	20.40383143825055	28.94919998923321	30.45001326622036	20.19695530629588
22	19.33022890959359	25.960262863427147	28.35356592157933	26.355942305399928
23	19.553255222853274	27.296113574227387	29.02649014262148	24.124141060297855
24	21.44013473865546	25.841059144271107	28.659265781995625	24.05954033507781
25	18.81765291722263	29.465236734740962	26.69317347217362	25.023936875862784
26	17.99668536755121	27.581817972075566	29.939744442607253	24.48175221776597
27	21.011385877820032	27.157683448755858	28.245128989959973	23.585801683464137
28	16.810031569759172	27.955579310848695	30.00319158344837	25.231197535943767
29	18.8438008298117	25.65879281240026	30.125471527614888	25.371934830173153
30	20.59878719828962	26.975417116885016	26.636263309479773	25.789532375345598
31	19.282162893804866	30.325041625169675	24.25026628572747	26.142529195297993
32	19.54710277283232	30.57498490727104	25.194667363944333	24.6832449559523
33	18.881484586190055	28.47853756263002	23.560422827127688	29.079555024052233
34	17.749049254207698	27.771390338346297	26.628572746953576	27.850987660492425
35	19.970468239899407	26.310183458369064	26.049857916857327	27.6694903848742
36	18.79996462341238	29.348724712469092	25.13468097624001	26.71662968787852
37	18.03013931454016	30.438092894304752	27.141533267450846	24.390234523704237
38	19.191414255995753	31.187922740608865	24.169515379202412	25.45114762419297
39	20.205414925074695	27.53951987818149	26.0290933980366	26.225971798707214
40	20.986391549609895	23.22280713222769	28.8584513514241	26.932349966738318
41	19.96470031800476	26.612807093774876	25.965646257195484	27.45684633102488
42	20.721451670582443	26.768156456804032	28.54006206283959	23.970329809773936
43	20.72914223310864	26.43246340253558	28.063247186215435	24.775147178140344
44	19.312925143909652	25.864130831849696	26.43515509941975	28.387788924820907
45	20.02161048069861	23.499667383170742	26.567048246744008	29.91167388938664
46	22.66331870844693	24.465986564587265	26.767771928677725	26.10292279828808
47	18.388904056387204	24.842824128370868	30.923751917834032	25.844519897407896
48	19.30138930012036	25.196205476449574	27.969422323395843	27.532982900034224
49	20.660696226625497	23.10014265993486	30.87184062078221	25.367320492657434
50	19.08797619001842	21.906182827742935	29.717487185600188	29.288353796638454
51	18.535409272511238	21.408987960424366	26.73585609419401	33.31974667287039
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	211.0
2	314.0
3	861.0
4	1408.0
5	965.0
6	522.0
7	521.0
8	520.0
9	553.0
10	586.0
11	618.0
12	650.0
13	645.0
14	640.0
15	636.0
16	632.0
17	595.5
18	559.0
19	569.0
20	579.0
21	619.5
22	660.0
23	708.5
24	757.0
25	910.0
26	1324.0
27	1585.0
28	1871.5
29	2158.0
30	2575.0
31	2992.0
32	3596.0
33	4200.0
34	4993.5
35	5787.0
36	6476.0
37	7165.0
38	7815.5
39	8466.0
40	9454.5
41	10443.0
42	12443.0
43	14443.0
44	16998.5
45	19554.0
46	25926.5
47	32299.0
48	35252.5
49	38206.0
50	36029.0
51	33852.0
52	27533.0
53	21214.0
54	17300.5
55	13387.0
56	11391.0
57	9395.0
58	8073.5
59	6752.0
60	6374.5
61	5997.0
62	5169.5
63	4342.0
64	3710.5
65	3079.0
66	2535.0
67	1991.0
68	1712.0
69	1433.0
70	1216.5
71	1000.0
72	781.0
73	562.0
74	470.0
75	270.0
76	162.0
77	137.0
78	112.0
79	88.5
80	65.0
81	41.5
82	18.0
83	18.5
84	19.0
85	11.0
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	260059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.42724659171229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.17273535088246	37.90754062512478
2	10.736528138422779	10.828271039502066
3	4.233390045145267	6.404346111763213
4	2.4845484508806552	5.011557496064624
5	1.6421429599411463	4.1404373989898255
6	1.3198973901862885	3.9935274702408887
7	1.0022162297941133	3.5377303470630763
8	0.775224824312007	3.1273962703678735
9	0.5728001576883079	2.5996261319567995
>10	1.96887021164849	14.109743704091372
>50	0.04735055790089221	1.6675795586671784
>100	0.03742221511522126	3.4110282104192144
>500	0.004582312054925052	1.861296075528516
>1k	0.002291156027462526	1.3999195602205992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	1470	0.5652563456754045	No Hit
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT	1145	0.44028470462471975	Illumina Single End Adapter 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC	1020	0.3922186888359949	No Hit
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	957	0.3679934168784776	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGC	871	0.33492399801583483	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	826	0.3176202323318939	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCG	814	0.31300589481617636	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC	701	0.269554216543169	No Hit
TCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	664	0.2553266758697065	No Hit
ACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	440	0.16919237557631153	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	439	0.16880784745000174	No Hit
ACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	357	0.13727654109259824	No Hit
AGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	320	0.12304900041913566	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCT	318	0.12227994416651605	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC	314	0.12074183166127686	No Hit
GCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	286	0.1099750441246025	No Hit
GGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	265	0.10189995347209672	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.845281263097989E-4	0.0	0.0	0.45451224529818235	0.0
2	3.845281263097989E-4	0.0	0.0	1.8734210313813404	0.0
3	3.845281263097989E-4	0.0	0.0	2.7835991063566343	0.0
4	3.845281263097989E-4	0.0	0.0	3.9575634759804506	0.0
5	3.845281263097989E-4	0.0	0.0	7.226821605866361	0.0
6	3.845281263097989E-4	0.0	0.0	8.841070680114896	0.0
7	3.845281263097989E-4	0.0	0.0	10.508000107667876	0.0
8	3.845281263097989E-4	0.0	0.0	13.011278209944667	0.0
9	3.845281263097989E-4	0.0	0.0	13.858778200331463	0.0
10	3.845281263097989E-4	0.0	0.0	16.126725089306657	0.0
11	3.845281263097989E-4	0.0	0.0	19.172956905932885	0.0
12	3.845281263097989E-4	0.0	0.0	21.6889244363779	0.0
13	3.845281263097989E-4	0.0	0.0	22.54565310179613	0.0
14	3.845281263097989E-4	0.0	0.0	22.885191437327684	0.0
15	3.845281263097989E-4	0.0	0.0	23.51889378948623	0.0
16	3.845281263097989E-4	0.0	0.0	24.72438946546745	0.0
17	3.845281263097989E-4	0.0	0.0	26.327102695926694	0.0
18	3.845281263097989E-4	0.0	0.0	27.92097177948081	0.0
19	3.845281263097989E-4	0.0	0.0	29.072248989652348	0.0
20	3.845281263097989E-4	0.0	0.0	30.04933495860555	0.0
21	3.845281263097989E-4	0.0	0.0	31.330198147343488	0.0
22	3.845281263097989E-4	0.0	0.0	32.67720017380671	0.0
23	3.845281263097989E-4	0.0	0.0	33.96652298132347	0.0
24	3.845281263097989E-4	0.0	0.0	34.95360668156072	0.0
25	3.845281263097989E-4	0.0	0.0	35.806490065715856	0.0
26	3.845281263097989E-4	0.0	0.0	36.599771590292974	0.0
27	3.845281263097989E-4	0.0	0.0	37.39959009301735	0.0
28	3.845281263097989E-4	0.0	0.0	38.19094897696292	0.0
29	3.845281263097989E-4	0.0	0.0	38.99653540158195	0.0
30	3.845281263097989E-4	0.0	0.0	39.882872732726035	0.0
31	3.845281263097989E-4	0.0	0.0	40.719990463702466	0.0
32	3.845281263097989E-4	0.0	0.0	41.505965953879695	0.0
33	3.845281263097989E-4	0.0	0.0	42.296940309698954	0.0
34	3.845281263097989E-4	0.0	0.0	43.02139129966661	0.0
35	3.845281263097989E-4	0.0	0.0	43.79044755228621	0.0
36	3.845281263097989E-4	0.0	0.0	44.55027512987437	0.0
37	3.845281263097989E-4	0.0	0.0	45.33971137318839	0.0
38	3.845281263097989E-4	0.0	0.0	46.0737755663138	0.0
39	7.690562526195978E-4	0.0	0.0	46.832449559523035	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	20	7.023209E-4	45.000004	28
TAAGTAG	20	7.023209E-4	45.000004	1
AGGCGAT	20	7.023209E-4	45.000004	6
TATGCGC	20	7.023209E-4	45.000004	22
AACGGGC	20	7.023209E-4	45.000004	4
GCCGATG	20	7.023209E-4	45.000004	9
TGCGCGC	20	7.023209E-4	45.000004	15
AGCGATG	20	7.023209E-4	45.000004	1
CTATTAG	20	7.023209E-4	45.000004	1
GTAAGCG	20	7.023209E-4	45.000004	1
CCTGCGT	20	7.023209E-4	45.000004	23
TACGTAG	20	7.023209E-4	45.000004	1
CCTCGTC	35	1.2070814E-7	45.0	36
CTACGAA	35	1.2070814E-7	45.0	11
TCGTCCC	35	1.2070814E-7	45.0	38
GCTACGA	35	1.2070814E-7	45.0	10
CCTATGC	35	1.2070814E-7	45.0	35
CAACGAG	30	2.159004E-6	44.999996	14
GCGCGAC	25	3.8824568E-5	44.999996	9
TGATTCA	30	2.159004E-6	44.999996	44
>>END_MODULE