FastQCFastQC Report
Sat 18 Jun 2016
SRR3550676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544016
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC17790.3270124408105644TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.1749948530925561No Hit
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT9180.1687450369106791TruSeq Adapter, Index 19 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC9100.16727449192670804TruSeq Adapter, Index 13 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC7150.13142995794241347No Hit
TCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC6550.12040087056263052TruSeq Adapter, Index 13 (95% over 22bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5850.10753360195288374No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5710.10496014823093437No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5520.10146760389400312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA453.8380676E-1045.0000045
CCGGTCA302.163386E-645.00000429
ACCCGGT302.163386E-645.00000427
AGTACGG453.8380676E-1045.0000042
GAATGCG253.888088E-545.01
CCGATCA207.0300064E-445.010
GCCGATT207.0300064E-445.09
CCCCGAT207.0300064E-445.040
ACAATTC207.0300064E-445.035
AGCGCAC207.0300064E-445.042
TGGGCGC502.1827873E-1145.06
TATACGG406.8030204E-945.02
TCGACGT253.888088E-545.026
TCTGGCG207.0300064E-445.036
GCGTAAG551.8189894E-1245.01
AACACGG502.1827873E-1145.02
TATGCGG800.042.18752
AATGCGG800.042.18752
CGGGTAT603.6379788E-1241.2500046
TAGACGG603.6379788E-1241.2500042
Produced by FastQC (version 0.11.5)