FastQCFastQC Report
Sat 18 Jun 2016
SRR3550675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006825
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29150.28952399870881235No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC13940.13845504432249894RNA PCR Primer, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT10910.10836043999702034RNA PCR Primer, Index 16 (95% over 24bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10680.10607603108782558No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC10640.10567874258187868RNA PCR Primer, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG302.1652322E-645.0000041
CGGTCTA750.045.031
TGCGTAG1050.040.7142871
CGCATCG403.4576442E-739.37500421
AACACGT1550.039.19354641
GCGATCA1100.038.8636369
CGTTTTT16850.038.456971
CCGTCGG301.1398212E-437.5000042
TACGGGA4500.037.54
GCAATAG552.748493E-936.818181
ATAGCGG1600.036.5625042
GCGAAAG1050.036.428571
TCAATCG250.002106984836.01
TCGTAAG250.002106984836.01
ATAACGC504.877984E-836.011
CAGTCGA250.002106984836.011
TTCGCAT250.002106984836.041
TAAGGGA7900.035.8860784
CACAACG1900.035.52631412
TTGTGCG1400.035.3571431
Produced by FastQC (version 0.11.5)