##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550672_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522913
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49305333774452	33.0	31.0	34.0	31.0	34.0
2	32.644548902016204	34.0	31.0	34.0	31.0	34.0
3	32.60082843608784	34.0	31.0	34.0	31.0	34.0
4	36.06948957092289	37.0	35.0	37.0	35.0	37.0
5	36.11785325666028	37.0	35.0	37.0	35.0	37.0
6	36.15217827822219	37.0	36.0	37.0	35.0	37.0
7	36.1195476111705	37.0	37.0	37.0	35.0	37.0
8	36.03609778299641	37.0	37.0	37.0	35.0	37.0
9	37.87915963076076	39.0	38.0	39.0	35.0	39.0
10	37.66406266434378	39.0	37.0	39.0	35.0	39.0
11	37.60230478110125	39.0	37.0	39.0	35.0	39.0
12	37.46274810532536	39.0	37.0	39.0	35.0	39.0
13	37.40126751486385	39.0	37.0	39.0	35.0	39.0
14	38.69183401445365	40.0	38.0	41.0	35.0	41.0
15	38.78371354317066	40.0	38.0	41.0	35.0	41.0
16	38.83954118562744	40.0	38.0	41.0	35.0	41.0
17	38.79666980931818	40.0	38.0	41.0	35.0	41.0
18	38.75511031471775	40.0	38.0	41.0	35.0	41.0
19	38.71127510694896	40.0	38.0	41.0	35.0	41.0
20	38.651211578216646	40.0	38.0	41.0	35.0	41.0
21	38.555147797052285	40.0	38.0	41.0	35.0	41.0
22	38.51024549016758	40.0	38.0	41.0	35.0	41.0
23	38.493202502137066	40.0	37.0	41.0	35.0	41.0
24	38.44953175767288	40.0	37.0	41.0	34.0	41.0
25	38.365498658476646	40.0	37.0	41.0	34.0	41.0
26	38.372629863858805	40.0	37.0	41.0	34.0	41.0
27	38.349782851067005	40.0	37.0	41.0	34.0	41.0
28	38.2786524718261	40.0	37.0	41.0	34.0	41.0
29	38.25445533004534	40.0	37.0	41.0	34.0	41.0
30	38.17492202335762	40.0	37.0	41.0	34.0	41.0
31	38.11676129681228	40.0	37.0	41.0	34.0	41.0
32	38.0276986802776	40.0	37.0	41.0	34.0	41.0
33	37.95494087926672	40.0	36.0	41.0	34.0	41.0
34	37.8431020074085	40.0	36.0	41.0	33.0	41.0
35	37.77793055441345	40.0	36.0	41.0	33.0	41.0
36	37.71121391130073	40.0	36.0	41.0	33.0	41.0
37	37.65494260039433	40.0	36.0	41.0	33.0	41.0
38	37.583376202159826	40.0	36.0	41.0	33.0	41.0
39	37.48549949991681	40.0	35.0	41.0	33.0	41.0
40	37.410970849835444	40.0	35.0	41.0	33.0	41.0
41	37.33663343615477	40.0	35.0	41.0	33.0	41.0
42	37.27333227515858	40.0	35.0	41.0	33.0	41.0
43	37.22122609305946	40.0	35.0	41.0	33.0	41.0
44	37.12816663575011	39.0	35.0	41.0	33.0	41.0
45	37.02020412573411	39.0	35.0	41.0	32.0	41.0
46	36.92945480414524	39.0	35.0	41.0	32.0	41.0
47	36.853396262858254	39.0	35.0	41.0	32.0	41.0
48	36.78081248697202	39.0	35.0	41.0	32.0	41.0
49	36.67112311225768	39.0	35.0	41.0	32.0	41.0
50	36.57286393721326	39.0	35.0	41.0	32.0	41.0
51	35.62172292522848	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	26.0
11	9.0
12	9.0
13	9.0
14	11.0
15	18.0
16	31.0
17	64.0
18	101.0
19	184.0
20	322.0
21	469.0
22	669.0
23	990.0
24	1516.0
25	2260.0
26	3051.0
27	3665.0
28	4196.0
29	4943.0
30	6003.0
31	7511.0
32	9412.0
33	13181.0
34	28105.0
35	41472.0
36	31716.0
37	49976.0
38	92345.0
39	220317.0
40	322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.71444580647259	23.379032458554292	25.072239550364973	11.834282184608147
2	33.15886199042671	25.7981729274277	23.29488844224565	17.748076639899946
3	32.132113755060594	24.139388387743278	25.7459653900362	17.982532467159928
4	30.314985475595368	25.90736891222823	24.477111871382046	19.300533740794357
5	26.008915441000703	30.643529612000464	23.388020569387262	19.959534377611572
6	27.734823957331333	34.0117763375552	23.04264762972043	15.210752075393039
7	80.45296253870147	7.032527399395311	7.75138502963208	4.763125032271143
8	81.1268796147734	6.66573598284992	7.803401330622877	4.40398307175381
9	73.62486685165601	9.580369965940797	10.076437189360371	6.7183259930428205
10	41.48873713218069	27.113305655051605	17.144725795686856	14.253231417080853
11	32.185851183657704	24.768364909650362	23.4987464453934	19.547037461298533
12	29.69786561053177	23.05584294136883	27.428463243407602	19.817828204691793
13	25.775989504946327	25.145100619032227	28.273728134508037	20.805181741513408
14	21.306794820553325	27.79219487754177	28.60035990690612	22.300650394998787
15	21.32228496901014	26.570959222662278	32.219317553780456	19.887438254547124
16	24.001315706436827	25.895703491785437	29.918361180540547	20.184619621237186
17	24.134225004924335	26.06054926919009	26.966244862912188	22.838980862973383
18	24.67465907330665	26.641334218120416	27.4678579419521	21.216148766620833
19	25.959958922421126	27.4280807706062	25.77579826854563	20.83616203842704
20	26.733127690457113	27.32117962261409	26.57210664106649	19.373586045862314
21	26.04639777553819	26.479739459527686	27.35426351993544	20.11959924499869
22	24.42643422519616	25.555876407738953	27.26514735720856	22.752542009856324
23	24.329668606441224	25.86797421368373	28.118444177138453	21.683913002736592
24	24.42509557039125	25.198646811228638	28.041184671255063	22.33507294712505
25	23.280928184994444	27.22517894946196	26.454878727436494	23.039014138107103
26	22.58521015924255	28.351943822394933	25.998779911763524	23.064066106598993
27	23.122393208812937	27.733676538927128	27.449881720286168	21.69404853197377
28	21.978417059816834	27.654313432636023	27.756433670610598	22.610835836936545
29	23.474268186103618	26.793367156678073	27.046181678405397	22.68618297881292
30	23.735688345862506	26.544951072166878	26.8105784327412	22.908782149229413
31	25.185833972381637	27.321944568216892	25.00205579130754	22.49016566809393
32	25.10819199369685	28.21253248628357	24.684985838944527	21.994289681075056
33	23.8140952701501	28.945350373771543	24.917338065796795	22.323216290281557
34	22.00672004712065	27.907127954363347	27.367076358782437	22.719075639733568
35	23.259509708115882	27.115982964661427	26.410320646073053	23.214186681149638
36	23.023141516848884	29.996385632026744	24.495470565849388	22.485002285274987
37	23.543686999558243	29.5829325337102	25.799129109431206	21.07425135730035
38	22.48940072249112	29.425736212333604	24.9705017851918	23.114361279983477
39	22.869387450684915	28.22821387114109	26.757414713346193	22.1449839648278
40	23.136735938865545	26.545524781368986	27.84478488773467	22.472954392030797
41	20.255950798698827	27.34776148231159	27.639588229782007	24.756699489207573
42	21.49458896604215	27.78416294871231	26.710561795174343	24.0106862900712
43	23.435064723959815	26.31489368212303	26.78036308143037	23.46967851248678
44	22.87818432511718	27.157481263613644	26.407643336463238	23.556691074805943
45	22.815076312885697	26.477062149917863	26.240502722250163	24.467358814946273
46	22.13427472638852	28.16931305972504	25.949058447581148	23.74735376630529
47	21.786224477111872	26.58013856989595	28.27583173491575	23.35780521807643
48	21.959102183345987	25.756865864876183	28.329186690711456	23.954845261066374
49	21.572804653929047	27.16379206483679	27.9530246905317	23.31037859070247
50	20.8436202580544	26.522002704082702	28.221138124315136	24.41323891354776
51	20.86370008012805	25.85955981205287	27.40302880211431	25.873711305704774
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	213.0
1	575.0
2	937.0
3	1601.0
4	2265.0
5	1626.5
6	988.0
7	957.5
8	927.0
9	955.0
10	983.0
11	1049.0
12	1115.0
13	1118.0
14	1121.0
15	1069.5
16	1018.0
17	1065.5
18	1113.0
19	1183.0
20	1253.0
21	1364.0
22	1475.0
23	1862.0
24	2249.0
25	2637.5
26	3788.0
27	4550.0
28	6002.0
29	7454.0
30	8588.5
31	9723.0
32	11073.0
33	12423.0
34	13958.0
35	15493.0
36	16068.0
37	16643.0
38	17676.0
39	18709.0
40	21552.5
41	24396.0
42	25960.5
43	27525.0
44	30664.5
45	33804.0
46	36260.0
47	38716.0
48	41283.0
49	43850.0
50	42960.0
51	42070.0
52	39253.5
53	36437.0
54	34113.5
55	31790.0
56	29797.0
57	27804.0
58	26859.5
59	25915.0
60	25268.0
61	24621.0
62	22616.0
63	20611.0
64	18010.5
65	15410.0
66	12823.5
67	10237.0
68	8474.5
69	6712.0
70	5354.0
71	3996.0
72	3121.5
73	2247.0
74	1812.0
75	1206.5
76	1036.0
77	681.0
78	326.0
79	269.0
80	212.0
81	160.0
82	108.0
83	66.5
84	25.0
85	15.5
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	522913.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.242490458300903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9512213712836	20.747904139545557
2	8.541611341743755	4.995559763189131
3	3.206599677750012	2.8130688144058635
4	1.8179452414583113	2.126449855082328
5	1.4150408084675654	2.068965866985959
6	1.2252717448584798	2.1497998384709875
7	1.0467996187332018	2.142771950379107
8	0.9413238767993595	2.2021323588380866
9	0.8320416926549772	2.189787413253453
>10	9.892223834337397	53.64109183504026
>50	0.08464536442840905	1.6729944620321198
>100	0.039369936943446066	2.0790107806076406
>500	0.005249324925792808	0.9753218087520662
>1k	6.56165615724101E-4	0.19514111341744075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1017	0.19448741951337986	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	930	0.17784985265235326	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	657	0.125642315260856	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	641	0.12258253284963275	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	624	0.11933151403770799	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	596	0.11397689481806725	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	549	0.10498878398509887	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	548	0.10479754758439741	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	538	0.10288518357738285	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03767357093818666	0.0
2	0.0	0.0	0.0	0.35512599610260215	0.0
3	0.0	0.0	0.0	0.6002910618018676	0.0
4	0.0	0.0	0.0	0.9192733781718948	0.0
5	0.0	0.0	0.0	1.797622166593678	0.0
6	0.0	0.0	0.0	2.3906462451688903	0.0
7	0.0	0.0	0.0	2.8826975041737346	0.0
8	0.0	0.0	0.0	3.589316004765611	0.0
9	0.0	0.0	0.0	3.8874535534591796	0.0
10	0.0	0.0	0.0	4.446437552709533	0.0
11	0.0	0.0	0.0	5.375655223717903	0.0
12	0.0	0.0	0.0	6.092409253546957	0.0
13	0.0	0.0	0.0	6.369893270964768	0.0
14	0.0	0.0	0.0	6.49362322221861	0.0
15	0.0	0.0	0.0	6.719855884248432	0.0
16	0.0	0.0	0.0	7.165627934283523	0.0
17	0.0	0.0	0.0	7.676802833358513	0.0
18	0.0	0.0	0.0	8.21130857331908	0.0
19	0.0	0.0	0.0	8.550753184564163	0.0
20	0.0	0.0	0.0	8.911425036287108	0.0
21	0.0	0.0	0.0	9.321435879391027	0.0
22	0.0	0.0	0.0	9.766442983823312	0.0
23	0.0	0.0	0.0	10.17741000893074	0.0
24	0.0	0.0	0.0	10.534448369040357	0.0
25	0.0	0.0	0.0	10.872936798281932	0.0
26	0.0	0.0	0.0	11.173369183783919	0.0
27	0.0	0.0	0.0	11.488718008540618	0.0
28	0.0	0.0	0.0	11.801198287286795	0.0
29	0.0	0.0	0.0	12.128977478089089	0.0
30	0.0	0.0	0.0	12.50762555147797	0.0
31	0.0	0.0	0.0	12.866193802793198	0.0
32	0.0	0.0	0.0	13.215008997672653	0.0
33	1.9123640070145513E-4	0.0	0.0	13.554836081719138	0.0
34	1.9123640070145513E-4	0.0	0.0	13.888543600943178	0.0
35	1.9123640070145513E-4	0.0	0.0	14.245773197453497	0.0
36	1.9123640070145513E-4	0.0	0.0	14.601664139158904	0.0
37	1.9123640070145513E-4	0.0	0.0	14.967116900899384	0.0
38	1.9123640070145513E-4	0.0	0.0	15.346147447089669	0.0
39	1.9123640070145513E-4	0.0	0.0	15.734931049715726	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	20	7.029757E-4	45.0	28
TATTGCG	20	7.029757E-4	45.0	1
ATTCACG	20	7.029757E-4	45.0	1
TGCGATG	35	1.2102282E-7	45.0	1
CTACGCG	20	7.029757E-4	45.0	1
GCGCGAC	70	0.0	41.785713	9
CGAATAT	130	0.0	41.53846	14
CGACCCG	50	1.0786607E-9	40.5	32
GTATGCG	50	1.0786607E-9	40.5	1
TACGAAT	140	0.0	40.17857	12
GGCGTAG	45	1.9248546E-8	40.0	1
ATTACGG	35	6.2417585E-6	38.571426	2
GGTATAC	35	6.2417585E-6	38.571426	8
ATGCGAG	35	6.2417585E-6	38.571426	1
GCTCGAG	35	6.2417585E-6	38.571426	1
AAGTACG	30	1.1390711E-4	37.499996	1
TGCGCAA	30	1.1390711E-4	37.499996	26
CCCTCGT	115	0.0	37.173916	14
CGTGGCG	55	2.743036E-9	36.81818	27
ACCCGCT	55	2.743036E-9	36.81818	34
>>END_MODULE