FastQCFastQC Report
Sat 18 Jun 2016
SRR3550671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537891
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8270.1537486219326964No Hit
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC8260.15356271066071006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG207.0299365E-445.01
TCGTTTA207.0299365E-445.038
GTCGAAG207.0299365E-445.01
CGACGGT207.0299365E-445.028
AAGCGTA207.0299365E-445.027
TCGTGCG207.0299365E-445.01
CCCCGAT253.8880284E-545.040
CGTAAGG650.044.9999962
CGTATGG800.042.18752
TCTACGG356.2420913E-638.571432
AGTTGCG356.2420913E-638.571431
AGTAGCG356.2420913E-638.571431
AACACGT700.038.5714341
TATGCGG659.094947E-1238.076922
CCATCGA659.094947E-1238.0769231
ACATACG950.037.89473717
TAACCGG301.1391144E-437.5000042
CGGATGG301.1391144E-437.5000042
CCCGATC301.1391144E-437.50000441
TACACGC1150.037.17391235