FastQCFastQC Report
Sat 18 Jun 2016
SRR3550668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705992
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34800.49292343256014237No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC10220.14476084714840962TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC7200.10198415846071911TruSeq Adapter, Index 22 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAA207.031435E-445.028
CCCGCGA207.031435E-445.028
TGTAACG502.1827873E-1144.9999961
CGTTTTT17500.041.271431
CGACCCG603.6379788E-1241.25000432
CGGTCTA556.002665E-1140.90909231
GTATGCG501.0804797E-940.4999961
GTAAGCG501.0804797E-940.4999961
AACACGT1000.040.49999641
GCGTAAG850.039.7058831
GTTCACG403.455698E-739.3751
AAACACG1150.039.13043240
TATTGCG750.039.01
TAGAACG356.2448635E-638.571431
TCGACGG356.2448635E-638.571432
TACGGGA3150.037.8571434
TATGCGG1550.037.7419362
GCGAGAC1200.037.50000421
ACACGAC1200.037.50000426
CGTAAGG1450.037.241382
Produced by FastQC (version 0.11.5)